Cloning of a cDNA encoding a human protein which binds a sequence in the c-myc gene similar to the interferon-stimulated response element

Stasiv, Yuri; Mashkova, T. D.; Chernov, B. K.; Sokolova, Irene V.; Itkes, A.V.; Kisselev, Lev L.

doi:10.1016/0378-1119(94)90018-3

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…C-myc promoter-binding protein may play a role in transcription regulation by recognizing an ISRE-like motif and binding to ISRE-like sequence of the p2 promoter region of c-myc. The NAG7, a tumor suppression candidate gene, may cause this protein in high abundance expression and bind to the ISRE-like sequences in c-myc, and play a role in the inhibition of the gene transcription initiating intron I [20].…”

Section: Ms and Protein Identificationmentioning

confidence: 99%

Proteomic analysis of differential protein expression in human nasopharyngeal carcinoma cells induced by NAG7 transfection

et al. 2002

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Nasopharyngeal carcinoma (NPC) is a commonly occurring tumor in southern China and south east Asia. A genetic factor has now been recognized to be associated with this cancer. A new gene, named NAG7, was cloned from the common minimal deletion region in 3p25.3-26.3. In order to investigate the function of NAG7 gene, proteomic methods were used to find and identify the differential proteins and expected to elucidate the mechanism of NAG7. The NAG7 eukaryotic expression vector was constructed and transfected into NPC cell line HNE1 with liposome. Twenty-two differential protein spots in transfected cells were found significant and reproducible using high-resolution two-dimensional electrophoresis. Nine proteins that were up-regulated and seven proteins that were down-regulated were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry and database analysis. These proteins included growth arrest specific protein, DNA binding protein, caspase 6, pinch protein and ras-related protein rab-36, which are involved in cell cycling, transcription regulation, signaling pathways and apoptosis. NAG7 may exert its functions by mediating differential expression of these proteins.

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Section: Ms and Protein Identificationmentioning

confidence: 99%

Proteomic analysis of differential protein expression in human nasopharyngeal carcinoma cells induced by NAG7 transfection

et al. 2002

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“…The c‐ myc oncogene is expressed in a variety of tissues and cultured cancer cell lines (Saito et al, 1991) and transcription of the c‐ myc gene is controlled by a number of regulatory sequences in the 5′‐nontranscribed region of this gene (Stasiv et al, 1994). However, regulation of the c‐ myc gene expression is not fully understood.…”

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confidence: 99%

Reduction of c‐myc expression by an antisense approach under Cre/loxp switching induces apoptosis in human liver cancer cells

Ebinuma

Saito

Kosuga³

et al. 2001

Journal Cellular Physiology

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c-Myc has been documented to be both a positive and a negative signal for the induction of apoptosis. It is well known that overexpression of the c-myc gene induces apoptosis of normal cells, but the result of a reduction in its expression is not fully understood. We examined whether a reduction in c-myc expression would induce apoptosis in human liver cancer cells. Specifically, antisense and sense oligodeoxynucleotides (oligos) against the human c-myc mRNA were synthesized, mixed with a liposome reagent at various ratios, and were applied to the liver cancer-derived cell lines, HCC-T, HepG2, and PLC/PRF/5. To exclude effects resulting from using oligos, plasmid vectors expressing the full-length c-myc cDNA in both sense and antisense orientations under the control of the Cre/loxP system were generated. Monoclonal cell lines including these plasmid vectors were produced and Cre was supplied by adenovirus infection. Apoptosis was determined morphologically and c-Myc and Bcl-2 expression was examined by Western blotting. The antisense myc significantly inhibited the proliferation of the cells within two days, while neither the liposome reagent alone nor sense myc did so. Most of the cells were rounded up by the antisense-treatment and nuclear fragmentation and DNA ladder formation were detected after two days in antisense c-myc-treated cells. Antisense c-myc largely reduced c-Myc and partially Bcl-2 expression; overexpression of Bcl-2 partially rescued from apoptosis in HCC-T and HepG2 cells. These results suggest that the massive reduction in c-myc mRNA induces apoptosis in liver cancer cell lines and consequent decrease in Bcl-2 enhances the cell death. c-Myc reduction under the Cre/loxP switching system may be a useful tool for the clarification of c-myc-related cellular mechanisms in differentiation and proliferation.

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“…34 DENND4A promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form, may bind to ISRE-like element (interferon-stimulated response element) of MYC P2 promoter. 35,36 Of 20 identified proteins, the molecular and cellular functions of all proteins have been documented in literatures, and several proteins were reported relating to cancer in previous studies. For instance, in addition to ENO1, the protein hits included key chaperone such as Protein/nucleic acid deglycase DJ-1 (PARK7), 60kD heat shock protein (HSPD1), protein disulfide-isomerase A6 (PDIA6), Calreticulin (CALR), Nucleophosmin (NPM1), Heat shock protein beta-1 (HSPB1), Additional candidate TAAs identified by our SERPA approach have been associated with Metal-binding, Hydrolase, Transferase, Isomerase, DNA-binding, cell motility, GTPbinding and so on.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunoseroproteomic profiling in autoantibody to ENO1 as potential biomarker in immunodiagnosis of osteosarcoma by serological proteome analysis (SERPA) approach

Dai

Huang

et al. 2021

OncoImmunology

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Osteosarcoma (OS) is the most common highly malignant primary solid bone tumor. Despite its relatively low incidence among cancers, it remains one of the most harmful primary malignant tumors in childhood and adolescence. It is now evident that serum autoantibodies against tumor-associated antigens (TAAs) could be used as serological cancer biomarkers in types of cancers. Serological proteome analysis (SERPA) approach was applied to profile anti-TAA autoantibody response in sera from patients with OS and normal human, as well as explore difference between this response. This approach can detect autoantibodies that could serve as clinical biomarkers and immunotherapeutic agents. Enzyme-linked immunosorbent assay (ELISA) and Western blotting were further used to validate the level of identified TAAs. ENO1 as a 47kD TAA in OS was identified and characterized by SERPA. Analysis of 172 serum samples with OS, osteochondroma (OC), and normal human sera (NHS) by ELISA showed higher frequency of anti-ENO1 autoantibodies in OS sera compared to others. Interestingly, decrease of ENO1 immunoreactivity was observed in most patients after treatments, which may imply a potential association between anti-ENO1 autoantibody titers and disease progression. Nine of twelve sera reacted strongly against purified ENO1, but three reacted weakly against purified ENO1, which indicated 75.0% sera with positive optimal density values from ELISA were consistently positive in Western blotting. The expression of ENO1 in OS tissues was evaluated by immunohistochemistry in tumor microarray. ENO1 was one of the autoantibodies that elicit autoimmune responses in OS and can be used as biomarkers in immunodiagnosis and progression of OS.

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Cloning of a cDNA encoding a human protein which binds a sequence in the c-myc gene similar to the interferon-stimulated response element

Cited by 8 publications

References 21 publications

Proteomic analysis of differential protein expression in human nasopharyngeal carcinoma cells induced by NAG7 transfection

Proteomic analysis of differential protein expression in human nasopharyngeal carcinoma cells induced by NAG7 transfection

Reduction of c‐myc expression by an antisense approach under Cre/loxp switching induces apoptosis in human liver cancer cells

Immunoseroproteomic profiling in autoantibody to ENO1 as potential biomarker in immunodiagnosis of osteosarcoma by serological proteome analysis (SERPA) approach

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