Cloning of a chitinase-like cDNA (hs2), its transfer to creeping bentgrass (Agrostis palustris Huds.) and development of brown patch (Rhizoctonia solani) disease resistant transgenic lines

GENETIC TRANSFORMATION OF COMMON BEANS (Phaseolus vulgaris L.) CALLI BY BIOLISTIC GUN AND VIA Agrobacterium tumefaciens

“…Wheat embryos transformed by bar gene germinated onto 5 mg l -l phosphinothricin medium expressed resistance, while control showed sensitivity . Callus culture media containing 5 and 10 mg l -l phosphinothricin were used for selection of transgenic embryogenic cultures of bentgrass (Chai et al, 2002). Differences were observed between the present results and the other researchers, may be due to different genotypes used in other plant families.…”

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

“…A procedure was developed to produce rice tolerant to the herbicide phosphinothricin by in vitro selection (Toldi et al, 2000). Chai et al (2002) produced transgenic bentgrass plants via biolistic system; 5 mg/l phosphinothricin inhibited the growth of non-transformed plantlets but did not affect the growth of transformed. Plasmid JS101 containing the bar herbicide resistance gene was used as a selectable marker, 1 mg/l zeatin and 0.1 mg/l IAA were enhanced shoot regeneration in tomato (Hussain et al, 2008).…”

Expression of a Chitinase Gene from Metarhizium anisopliae in Tobacco Plants Confers Resistance against Rhizoctonia solani

“…Strong degrees of variation on chitinase transgene expression among transgenic plants independently produced have been reported in other studies, including groundnut [35], cucumber [36], potato and tobacco [8], and creeping grass [37]. This pattern has been associated to intrinsic properties of the transformation protocols which may give rise to epigenetic silencing, co-suppression, position effects, and changes in transgene structure [38,39].…”

“…In those transgenic plants harboring 1, 3, and 4 chit1 copies, enzyme levels were much lower. No correlation between the number of copies, and the level of enzyme production was observed in transgenic plants transformed with chitinase genes from Trichoderma [8] or American elm [37].…”