Cloning of a gene responsible for the specific production of an antifungal antibiotic iturin with n-C16-.BETA.-amino acid residue.

Matsuno, Yuko; Ano, Takashi; Shoda, Makoto

doi:10.2323/jgam.38.505

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Iturins have been frequently cited for the antagonistic activity to other microorganisms, mainly plant pathogenic fungi (McKeen et al 1986;Besson & Michel 1987;Matsuno et al 1992), but they may also be effective against bacteria (Maget-Dana & Peypoux 1994;Bernal et al 2002). In one study on iturins, Phae & Shoda (1991) demonstrated that five HPLC peaks showed antagonistic activity to eight phytopathogens, and those peaks were related to peaks 1-5 described by Matsuno et al (1992). Furthermore, peak 6 of the study of Phae & Shoda (1991) was identified as comprising the chain g-C 17 -b-amino acid.…”

Section: Discussionmentioning

confidence: 97%

“…The supernatant containing the solubilized antibiotics was subsequently collected as methanol extract. The technique described by Matsuno et al (1992) was used for the identification of antibiotic compounds. From the methanol extract, a 20-ll aliquot was drawn and injected into the HPLC (Millipore, Waters model 570), with photodiode detector, equipped with a C-18 reverse phase column (10.7 mm diameter · 250 mm), and the mobile phase consisted of acetonitrile,10 mM of ammonium acetate, (2:3, v:v) at a 1 ml min )1 flow.…”

Section: Detection Of Antibiotics Produced By the Bacillus Strainsmentioning

confidence: 99%

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Phytohormones and Antibiotics Produced by Bacillus subtilis and their Effects on Seed Pathogenic Fungi and on Soybean Root Development

Araújo

Henning

Hungría

2005

World J Microbiol Biotechnol

138

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Bacteria belonging to the genus Bacillus were isolated from soil samples of Parana´State, Brazil, with the aim of evaluating their potential biological control of soybean seed pathogens. Strain PRBS-1 was selected, showing similar effectiveness to that of the strain AP-3, used as a reference due to its known antibiotic potential. The sequencing of the ribosomal 16S rRNA gene confirmed that both strains belong to the species B. subtilis, although showing high genetic diversity in relation to this species. Both strains inhibited five soybean seed pathogenic fungi in vitro, Rhizoctonia solani, Colletotrichum truncatum, Sclerotinia sclerotiorum, Macrophomina phaseolina and Phomopsis sp. Furthermore, the metabolites of AP-3 increased production of root hairs, while the metabolites of PRBS-1 stimulated outgrowth of lateral roots in soybean. The antibiotic effect of both strains seemed to be related to compounds of the iturin group, while the root growth promotion by PRBS-1 was at least partially related to the production of indoleacetic acid. The results have shown the potential of using selected strains of B. subtilis in the biological control of seed pathogens, as well as in promoting soybean growth.

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Section: Discussionmentioning

confidence: 97%

Section: Detection Of Antibiotics Produced By the Bacillus Strainsmentioning

confidence: 99%

Phytohormones and Antibiotics Produced by Bacillus subtilis and their Effects on Seed Pathogenic Fungi and on Soybean Root Development

Araújo

Henning

Hungría

2005

World J Microbiol Biotechnol

138

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“…A atração de Bacillus por exsudatos radiculares tem sido estudada na espermosfera (FUKUI et al, 1994); na rizosfera esta atração pode estar relacionada com produção de hormônios por estirpes de Bacillus (ARAÚJO, 1995). Bacillus subtilis é um agente eficiente de controle biológico sendo utilizado freqüentemente no controle de fitopatógenos de solo, sendo esse efeito devido, principalmente, à produção de antibióticos (MATSUNO et al, 1992). Esse mecanismo desenvolvido na rizosfera influencia outros habitantes de solo de forma positiva ou negativa.…”

Section: Resultsunclassified

Influência De Bacillus Subtilis Na Eclosão, Orientação E Infecção De Heterodera Glycines Em Soja

2002

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A utilização de microrganismos para fins de controle biológico de doenças em plantas tem sido muito estudada no mundo. No caso do nematóide de cisto da soja (Heterodera glycines), uma das principais doenças da soja, o controle biológico é de grande importância devido à ausência de controle químico eficiente. Para se estudar o efeito de Bacilllus subtilis sobre H. glycines, foram conduzidos alguns experimentos de casa de vegetação e laboratório. Nos trabalhos de laboratório, verificou-se, em câmara de eclosão, que a presença de B. subtilis reduz a eclosão de ovos de H. glycines estimulados com exsudatos de sementes de soja. Foi observado também que o tratamento de raiz de soja com a bactéria inibiu a migração de larvas juvenis de H. glycines para a planta em comparação à raiz não tratada com a bactéria. Nos ensaios de casa de vegetação, utilizando-se vasos com solo infestados com ovos de H. glycines, observou-se uma redução de fêmeas na raiz de soja quando o solo ou sementes foram tratadas previamente com formulação pó-molhável ou calda contendo B. subtilis, respectivamente. Com base nesses resultados e sabendo-se que H. glycines apresenta dependência de estímulo de exsudatos vegetais para eclosão e orientação das larvas, pode-se afirmar que B. subtilis interfere nesse estímulo prejudicando o desenvolvimento do ciclo do nematóide.

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“…It was originally hoped that iturin may prove to protect stored grains from aflatoxin contamination caused by species of Aspergillus flavus or parasiticus. While some of the aflatoxin producing strains in our laboratory proved to be sensitive to high concentrations of iturin, we have found that some of the producing strains are not sensitive to the toxin and that in some cases the synthesis of the aflatoxin in liquid culture at least is increased by the iturin (29). It is questionable whether iturin can be used to control all species of Aspergilli and their toxins.…”

Section: Although Naturally Occurring Iturins Contain a Mixture Of Eimentioning

confidence: 94%

Biorational Control of Weeds and Fungi with Peptides

Lax

Bland

Shepherd

1993

Natural and Engineered Pest Management Agents

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Tentoxin is a cyclic tetrapeptide produced by the fungus Alternaria alternata that disrupts chloroplast development in most of the major weed species in soybean and johnsongrass in corn while having no effect on either of these crop species. The major impediment to the development of tentoxin as a herbicide is its limited availability because of low biosynthetic yields. Iturins are a family of cyclic octapeptides having a unique β-amino acid, iturinic acid, incorporated into the peptide backbone. Iturins have a broad antifungal activity, but low biosynthetic yields may limit their commercialization. Chemical synthesis of both molecules is possible and the production of analogues has led to meaningful structure/activity relationships. To date no analog has provided greater activity or specificity than the parent molecule for either of the two agents. Molecular genetic manipulation is considered the best strategy for the increase in production of both of these compounds for cost effective synthesis. Success of cloning of the biosynthetic genes may determine the eventual deployment of these biorational pesticides.The cost of pest control in agriculture is staggering; direct costs of pesticides are estimated to be at least $20 billion annually, with unestimated indirect costs making the exact amount impossible to accurately state. Recent popular concern for the environment and legislation toward banning some of the more traditional pesticide compounds has led to the development of new strategies for the control of pests which include microbes as biological control organisms and the use of the biorational approach in which natural products provide a basis for the This chapter not subject to U.S.

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Cloning of a gene responsible for the specific production of an antifungal antibiotic iturin with n-C16-.BETA.-amino acid residue.

Cited by 6 publications

References 14 publications

Phytohormones and Antibiotics Produced by Bacillus subtilis and their Effects on Seed Pathogenic Fungi and on Soybean Root Development

Phytohormones and Antibiotics Produced by Bacillus subtilis and their Effects on Seed Pathogenic Fungi and on Soybean Root Development

Influência De Bacillus Subtilis Na Eclosão, Orientação E Infecção De Heterodera Glycines Em Soja

Biorational Control of Weeds and Fungi with Peptides

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