Cloning of a novel endogenous promoter for foreign gene expression in Phaeodactylum tricornutum

Erdene-Ochir, Erdenedolgor; Shin, Bok-Kyu; Huda, Nazmul; Kim, Da Hye; Lee, Eun Ha; Song, Dae-Geun; Kim, Yoon-Mi; Kim, Sang Min; Pan, Cheol‐Ho

doi:10.1007/s13765-016-0235-y

Cited by 17 publications

(27 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…tricornutum genome has a relatively higher gene density than other higher eukaryotic genomes 32 . Approximately, 500-bp upstream regions of endogenous genes are sufficient for full promoter activity by strongly driving transgene expression 7,12,15,19,22,33 . Therefore, we considered the 499-bp upstream region before the start codon of the HASP1 gene as the potential promoter.…”

Section: Resultsmentioning

confidence: 99%

“…3b). It is well known that the fcpA promoter is primarily active during the log phase 19 . Notably, the HASP1 promoter activity was higher than that of the fcpA promoter during the same phase.…”

Section: Resultsmentioning

confidence: 99%

“…We have therefore demonstrated that—compared with a previously reported promoter—the HASP1 promoter is more active in P . tricornutum 7,11,19,24,41–45 . Moreover, the transcriptional level of SP-GFP in the HASP1pro:SP-GFP line was 3 and 147-fold higher than that of the fcpApro:SP-GFP line on day 4 and 8, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…tricornutum for a variety of different applications 14–18 . However, the fcpA promoter is light-inducible and is not always able to drive constitutive expression of downstream transgenes throughout all growth phases of the culture 19–21 . The nitrate reductase gene promoter has also been widely used as a strong endogenous promoter for many purposes, such as the heterologous expression of human antibodies 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, other endogenous promoters such as V-ATPase C and Pt211 have been identified as strong promoters 22,23 . On the other hand, heterologous promoters, such as the CIP1 promoter of the putative replication-associated protein gene from the Chaetoceros lorenzianus -infecting DNA virus, are less active than the diatom endogenous gene promoter 19,24 .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum

Erdene-Ochir

Shin²,

Kwon³

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted protein in Phaeodactylum tricornutum , designated ‘highly abundant secreted protein 1’ (HASP1), and characterised the activities of its promoter and signal peptide using green fluorescent protein (GFP) as a reporter. The HASP1 promoter strongly drove GFP expression during all growth phases of P . tricornutum in culture, in contrast to the commonly used fcpA promoter, which is less active during the stationary phase. The HASP1 signal peptide was also sufficient for facilitating efficient secretion of GFP by P . tricornutum . Our findings suggest that both the promoter and the signal peptide of HASP1 can be utilized as novel tools for the overexpression and secretion of recombinant proteins in P . tricornutum .

show abstract