Cloning of a novel glutamate receptor subunit, GluR5: Expression in the nervous system during development

Bettler, Bernhard; Boulter, Jim; Hermans‐Borgmeyer, Irm; O'Shea-Greenfield, A; Deneris, Evan S.; Moll, Carlo; Borgmeyer, Uwe; Hollmann, Michael; Heinemann, Stephen F.

doi:10.1016/0896-6273(90)90213-y

Cited by 612 publications

(376 citation statements)

References 38 publications

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“…In the absence of biochemical data on this invertebrate subunit, it is difficult to draw definite conclusions about the exact number and locations of membrane-spanning domains; however, for simplicity, we assume that the topology of this polypeptide is similar to that proposed for vertebrate glutamate receptor subunits [3,4]. The mature Lymnaean polypeptide displays significant identity to rat [3][4][5][6]8] glutamate receptor subunits (GluRI, 43%: GIuR2, 46%; GIuR3, 45%; GIuR4, 43%; GluR5, 37% and GIuR6, 39%); in particular, it exhibits pronounced similarity to vertebrate sequences around TM3 (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

“…3. Alignment of the amino-acid sequence of the molluscan polypeplide with those of three rat glutamate receptor subunits, The sequences o|" GIuRI (AMPA-selective: [3]), GIuRS-2 (one form of GluR5 that is responsive to L-glutamate only; [6]). GIuR6 (KA-selective; [8] other is found in the C-terminal 79 amino acids which occur after TM4, a region that has also been assigned an extracellular location in vertebrates [3,4].…”

Section: Resultsmentioning

confidence: 99%

“…In this context, it is noteworthy that two vertebrate homo-oligomeric glutamate receptors (GIuR2 and GIuR5) show only very small responses to agonists when their corresponding cDNAs are expressed singly in Xenopus oocytes [5][6][7] and that coexpression studies [5,7] indicate that vertebrate glutamate receptors exist in vivo as hetero-oligomers.…”

Section: Voluroe 292 Number 12 Febs Le'i'rers November 1991mentioning

confidence: 99%

“…Recently, molecular biological studies have led to the isolation of 6 different vertebrate non-NMDA ionotropic glutamate receptor subunit cDNAs [3][4][5][6][7][8]. When these cDNAs were expressed singly either in Xenopus oocytes [3,[5][6][7][8] or in mammalian cells [4], homo-oligo-meric receptors were formed that were AMPA-selective (GIuRI to GluR4), KA-selective (GIuR6) or responsive to L-glutamate alone (GIuR5).…”

Section: Introductionmentioning

confidence: 99%

“…When these cDNAs were expressed singly either in Xenopus oocytes [3,[5][6][7][8] or in mammalian cells [4], homo-oligo-meric receptors were formed that were AMPA-selective (GIuRI to GluR4), KA-selective (GIuR6) or responsive to L-glutamate alone (GIuR5). No sequence has yet been reported for any invertebrate glutamate receptor.…”

Section: Introductionmentioning

confidence: 99%

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Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit

1991

FEBS Letters

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A full-length eDNA which encodes a putative glutamate receptor polypeptide was isolated from the pond snail Lymnaea stagnalis, using a short stretch of exonic sequence and two variants of the polymerase chain reaction. In this first comparison of invertebrate and vertebrate glutamate receptor sequences, the mature molluscan polypeptide, which comprises 898 amino acids and has a predicted M~ of 100 913, displays between 37% and 46% amino-acid identity to the rat ionotropie glutamate receptor subunits, GIuRI to GIuR6.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Voluroe 292 Number 12 Febs Le'i'rers November 1991mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit

1991

FEBS Letters

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Colocalization of ionotropic glutamate receptor subunits with NADPH-diaphorase-containing neurons in the rat mesopontine tegmentum

Inglis

Semba

1996

J. Comp. Neurol.

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Tegmental cholinergic neurons vary their discharge patterns across the sleep-wake cycle, and glutamate is suggested to play an important role in determining these firing patterns. Cholinergic and noncholinergic neurons in the mesopontine tegmentum have different susceptibilities to various excitotoxins, presumably because of heterogeneity in the expression of glutamate receptor subtypes in this area. By using a double-labeling procedure that combines nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-diaphorase) histochemistry and avidin-biotin-peroxidase immunocytochemistry with diaminobenzidine as the chromogen, we compared the colocalization of AMPA receptor subunits GluR1, GluR2/3, and GluR4, kainate receptor subunits GluR5/6/7, and an NMDA receptor subunit NMDAR1 on NADPH-diaphorase-positive (cholinergic) neurons in the mesopontine tegmentum. Throughout the brainstem, neurons immunoreactive for GluR2/3 and NMDAR1 were most numerous, whereas neurons labeled for GluR1, GluR4, and GluR5/6/7 were less common. Specifically within the mesopontine tegmentum, the proportion of double-labeled neurons in the diaphorase-containing cell population was highest with GluR1 (43%) and lowest with GluR5/6/7 (12%). Regardless of the receptor subunit type, the greatest numbers of double-labeled neurons were observed in the pedunculopontine tegmental nucleus pars compacta and the fewest in the dorsal aspect of the laterodorsal tegmental nucleus. In addition, there were regional differences in the relative expression of receptor subunits and diaphorase-positive neurons across the subdivisions of the tegmental cholinergic column. Because each ionotropic subunit confers distinctive properties to a receptor channel, the present results suggest that mesopontine cholinergic neurons have nonuniform responses to glutamate and are also discriminable from basal forebrain cholinergic neurons in terms of glutamate receptor configuration.

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Expression of glutamate receptors in the human and rat basal ganglia: Effect of the dopaminergic denervation on AMPA receptor gene expression in the striatopallidal complex in parkinson's disease and rat with 6-OHDA lesion

et al. 1996

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The overactivity of subthalamopallidal and corticostriatal glutamatergic neurons observed in Parkinson's disease (PD) suggests that antagonists of glutamate receptor could be used to alleviate the motor symptoms of the disease. In this study, we analysed two features of the striatopallidal complex: (1) the distribution of alpha-amino-3 hydroxy-5-methyl-4-isoxasol-propionate (AMPA) and kainate receptors and their corresponding mRNA by immunohistochemistry and in situ hybridisation and (2) the effect of dopaminergic denervation on AMPA receptor gene expression in PD patients and rats with 6-hydroxydopamine (6-OHDA)-induced degeneration of the nigrostriatal dopaminergic system. All AMPA receptor mRNAs and proteins (GluR1-4) were detected in the internal segment of the globus pallidus (GPi). Among kainate receptors, only KA1 and KA2 were detectable and only at a low level. Only GluR4 protein was detected in the neuropil of the GPi. In the striatum, GluR1, GluR2, and GluR3 were detected in about 70% of medium-sized and large neurons. By contrast, GluR4 mRNA was detected in only a small number of large and medium-sized neurons. Among kainate receptors, GluR6, GluR7, and KA2 were detected in about 50-60% of medium-sized neurons, whereas GluR5 and KA1 were restricted to 1-2% and 20-30% of these neurons, respectively. These results suggest that antagonists of AMPA and kainate receptors could be effective in alleviating motor symptoms in Parkinson's disease by blocking the overstimulation of pallidal and striatal neurons by glutamate. A significant decrease in GluR1 gene expression (-33%) was observed in the neurons of the GPi in PD patients and in rat entopeduncular nucleus ipsilateral to the 6-OHDA lesion (-20%). GluR2, GluR3, and GluR4 mRNA levels in the GPi and GluR1-4 levels in the striatum were unchanged in PD patients and 6-OHDA-lesioned rats compared with their respective controls. These data suggest that dopamine positively regulates only GluR1 gene expression in the GPi.

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Cloning of a novel glutamate receptor subunit, GluR5: Expression in the nervous system during development

Cited by 612 publications

References 38 publications

Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit

Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit

Colocalization of ionotropic glutamate receptor subunits with NADPH-diaphorase-containing neurons in the rat mesopontine tegmentum

Expression of glutamate receptors in the human and rat basal ganglia: Effect of the dopaminergic denervation on AMPA receptor gene expression in the striatopallidal complex in parkinson's disease and rat with 6-OHDA lesion

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