Cloning of a Tobacco Apoplasmic Invertase Inhibitor1

Greiner, Steffen; Krausgrill, Silke; Rausch, Thomas

doi:10.1104/pp.116.2.733

Cited by 112 publications

(49 citation statements)

References 44 publications

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“…Similar Southern banding patterns have been seen with other N. tabacum genes, e.g. the gene for apoplasmic invertase inhibitor (Greiner et al 1998).…”

Section: Discussionsupporting

confidence: 60%

Isolation and characterisation of a full-length genomic clone encoding a plastidic glucose 6-phosphate dehydrogenase from Nicotiana tabacum

Knight

Emes

Debnam

2001

Planta

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We describe here the isolation and characterisation of the first full-length genomic clone encoding a plant glucose 6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) from Nicotiana tabacum L. cv Samsun. The gene was expressed in all tissues, including roots, leaves, stems and flowers. Comparison of the gene with other known plant G6PDH cDNAs grouped this sequence with plastidic isoforms. The protein, minus a putative plastidic transit sequence, was overexpressed in Escherichia coli as a glutathione S-transferase fusion protein. The resulting protein was shown to be immunologically related to the potato plastidic G6PDH. This suggests that the sequence described here codes for a plastidic isoform. Plastidic G6PDH mRNA was induced in both roots and leaves in response to KNO3, and the induction in roots was approximately 4 times the response seen in leaves. Sequence analysis of the 5'-untranslated region of the genomic clone indicated the presence of several NIT2 elements, which may contribute to the control of the expression of this gene. Plastidic G6PDH mRNA levels did not appear to respond to light.

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“…Similar Southern banding patterns have been seen with other N. tabacum genes, e.g. the gene for apoplasmic invertase inhibitor (Greiner et al 1998).…”

Section: Discussionsupporting

confidence: 60%

Isolation and characterisation of a full-length genomic clone encoding a plastidic glucose 6-phosphate dehydrogenase from Nicotiana tabacum

Knight

Emes

Debnam

2001

Planta

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“…A 20-kDa protein is identified as a wall-modulating protein previously studied as an inhibitor of pectin methylesterase (29) or inhibitor of invertase (30) in non-pollen tissues (Table I). The common features of these two enzymes include the presence of a sugar-binding motif and the reversibility of the enzymic reactions.…”

Section: The Pollen-released Proteins Could Loosen or Form New Tube Wmentioning

confidence: 99%

Cell Wall Reactive Proteins in the Coat and Wall of Maize Pollen

Suen

Chang

et al. 2003

Journal of Biological Chemistry

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The surface of a pollen grain consists of an outermost coat and an underlying wall. In maize (Zea mays L.), the pollen coat contains two major proteins derived from the adjacent tapetum cells in the anthers. One of the proteins is a 35-kDa endoxylanase (Wu, S. S. H., Suen, D. F., Chang, H. C., and Huang, A. H. C. (2002) J. Biol. Chem. 277, 49055-49064). The other protein of 70 kDa was purified to homogeneity and shown to be a ␤-glucanase. Its gene sequence and the developmental pattern of its mRNA differ from those of the known ␤-glucanases that hydrolyze the callose wall of the microspore tetrad. Mature pollen placed in a liquid medium released about nine major proteins. These proteins were partially sequenced and identified via GenBank TM data bases, and some had not been previously reported to be in pollen. They appear to have wall-loosening, structural, and enzymatic functions. A novel pollen wall-bound protein of 17 kDa has a unique pattern of cysteine distribution in its sequence (six tandem repeats of CX 3 CX 10 -15 ) that could chelate cations and form signal-receiving finger motifs. These pollen-released proteins were synthesized in the pollen interior, and their mRNA increased during pollen maturation and germination. They were localized mainly in the pollen tube wall. The pollen shell was isolated and found to contain no detectable proteins. We suggest that the pollen-coat ␤-glucanase and xylanase hydrolyze the stigma wall for pollen tube entry and that the pollen secrete proteins to loosen or become new wall constituents of the tube and to break the wall of the transmitting track for tube advance.In plants, sexual reproduction is initiated when the pollen grain (male component) lands on the stigma of the style (female component) in the flowers (for reviews, see Refs.

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“…In a photoautotrophic cell culture of Chenopodium rubrum, cell-wall invertase enzyme activity is up-regulated by suc or glc (2,9). Another mode of control is through an invertase inhibitor protein by proteinprotein interactions (10,11). In contrast to our lack of understanding on the regulatory controls of plant invertase genes, there is a wealth of knowledge on a single invertase gene, sucrase2 (suc2), in yeast.…”

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confidence: 99%

Sugars modulate an unusual mode of control of the cell-wall invertase gene ( Incw 1) through its 3′ untranslated region in a cell suspension culture of maize

Cheng

Taliercio

Chourey

1999

Proc. Natl. Acad. Sci. U.S.A.

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We show here that a cell-wall invertase encoded by the Incw1 gene is regulated at both the transcriptional and posttranscriptional levels by sugars in a heterotrophic cell suspension culture of maize. The Incw1 gene encoded two transcripts: Incw1-S (small) and Incw1-L (large); the size variation was attributable to different lengths in the 3 untranslated region. Both metabolizable and nonmetabolizable sugars induced Incw1-L RNA apparently by default. However, only the metabolizable sugars, sucrose and D-glucose, were associated with the increased steady-state abundance of Incw1-S RNA, the concomitant increased levels of INCW1 protein and enzyme activity, and the downstream metabolic repression of the sucrose synthase gene, Sh1. Conversely, nonmetabolizable sugars, including the two glucose analogs 3-O-methylglucose and 2-deoxyglucose, induced greater steady-state levels of the Incw1-L RNA, but this increase did not lead to either an increase in the levels of the INCW1 protein͞enzyme activity or the repression of the Sh1 gene. We conclude that sugar sensing and the induction of the Incw1 gene is independent of the hexokinase pathway. More importantly, our results also suggest that the 3 untranslated region of the Incw1 gene acts as a regulatory sensor of carbon starvation and may constitute a link between sink metabolism and cellular translation in plants.The enzyme invertase (EC 3.2.1.26), also known as ␤-fructofuranosidase, is known to catalyze sucrose (suc) cleavage to its monosaccharide constituents, glucose (glc) and fructose, in an irreversible manner. At least two forms of the enzyme, soluble and particulate, are features common to all invertases, and recent molecular analyses indicate that each belongs to two distinct class of small gene families (ref. 1 and references therein). The physiological role of the invertases is believed to be in suc partitioning between source and sink regions of the plant. In general, the vacuolar invertases are believed to be important in the regulation of hexose levels in certain specific tissues and in the use of stored suc in vacuoles. The cell-wall forms (extracellular or secreted) are associated with rapidly growing tissues and have been implicated in phloem unloading and source͞sink regulation (2).Although the experimental evidence is largely correlative in nature, a lot of insight regarding the roles of invertases is now emerging from studies of transgenic or mutant plants. Among several studies on transgenic plants, the most detailed analyses are reported in potato where yeast invertase has been expressed in both the cytosol and apoplast (3). Tissue-specific elevated expression of invertase in both cellular locations leads to substantial reductions in tuber suc content and a corresponding large increase in glc content. More importantly, the apoplastic and cytosolic increases in enzyme activities are associated with different phenotypic responses: an increased tuber size but reduced tuber number in the former and a reversed pattern in the latter. In contrast, w...

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Cloning of a Tobacco Apoplasmic Invertase Inhibitor1

Cited by 112 publications

References 44 publications

Isolation and characterisation of a full-length genomic clone encoding a plastidic glucose 6-phosphate dehydrogenase from Nicotiana tabacum

Isolation and characterisation of a full-length genomic clone encoding a plastidic glucose 6-phosphate dehydrogenase from Nicotiana tabacum

Cell Wall Reactive Proteins in the Coat and Wall of Maize Pollen

Sugars modulate an unusual mode of control of the cell-wall invertase gene ( Incw 1) through its 3′ untranslated region in a cell suspension culture of maize

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