Cloning of a turkey prolactin cDNA: Expression of prolactin mRNA throughout the reproductive cycle of the domestic turkey (Meleagris gallopavo)

Wong, Eric A.; Ferrin, Neal H.; Silsby, J.L.; Halawani, Mohamed E. El

doi:10.1016/0016-6480(91)90101-b

Cited by 68 publications

(39 citation statements)

References 28 publications

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“…Previously, high levels of PRL expression were observed in response to exogenous VIP (Talbot et al 1991, Pitts et al 1994b, Xu et al 1996 and under conditions of an enhanced VIP secretion (Wong et al 1991, Youngren et al 1996. In contrast, VIP immunoneutralization was shown to suppress PRL mRNA steady-state concentrations (Youngren et al 1994).…”

Section: Discussionmentioning

confidence: 95%

“…The 3 -untranslated region of mRNA with an AU-rich domain has been shown to destabilize or stabilize mRNA transcripts (Bickel et al 1990, Iwai et al 1991. Analysis of the turkey PRL mRNA has revealed the presence of such an AU-rich domain (Wong et al 1991, Kurima et al 1995. Furthermore, activation of protein kinase C has been shown to stabilize mRNA transcripts by acting on an AU-rich domain in the 3 -untranslated region (Iwai et al 1991).…”

Section: Discussionmentioning

confidence: 99%

“…One microgram cytoplasmic RNA from each sample was immobilized on a nitrocellulose membrane in duplicate using a slot blot manifold (Scheicher & Schuell Inc., Keene, NH, USA). PRL mRNA was quantified by hybridization to a nick-translated turkey PRL cDNA probe (Wong et al 1991). The membrane was prehybridized for 5 h, then hybridized at 42 C for 16 h, followed by washing once with 2 SSC for 5 min at room temperature, twice with 2 SSC and 1% SDS for 30 min at 65 C, once with 0·1 SSC and 1% SDS for 30 min at room temperature.…”

Section: Slot Blot Analysismentioning

confidence: 99%

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Vasoactive intestinal peptide stimulates turkey prolactin gene expression by increasing transcription rate and enhancing mRNA stability

Tong

Pitts

You

et al. 1998

Journal of Molecular Endocrinology

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This study evaluates the transcriptional and post-transcriptional regulation of prolactin (PRL) by vasoactive intestinal peptide (VIP). Pituitary nuclei from laying (control), incubating (with enhanced VIP secretion), and VIP-immunized laying turkey hens, and from pituitary cells cultured with or without VIP were used in nuclear run-on transcription assays. Cytoplasmic PRL mRNA was analyzed by slot blot hybridization. PRL transcription was greater in hyperprolactinemic incubating birds (PRL/ -actin=3·33) than in laying birds (PRL/ -actin=1·83). VIP-immunoneutralized birds had 47% and 51% decreases in PRL transcription and cytoplasmic PRL mRNA, respectively when compared with laying birds. In primary pituitary cell cultures, VIP significantly increased the transcription rate of PRL (3·8-fold) and cytoplasmic PRL mRNA (3·2-fold) compared with that of non-VIP-treated pituitary cells. The stability of pre-existing PRL mRNA was measured by Northern blot analysis after addition of actinomycin D. PRL mRNA half-lives were calculated using a two-component model, with a first-long component of 18·0 1·0 h and a second-short component of 3·7 0·7 h in non-VIP-treated pituitary cells. Both half-lives were significantly increased (53·2 6·9 and 26·3 4·3 h) in VIP-treated cells. The present data show that VIP acts to stimulate PRL expression by up-regulating the transcription rate of PRL and by enhancing PRL mRNA stability.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Slot Blot Analysismentioning

confidence: 99%

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Vasoactive intestinal peptide stimulates turkey prolactin gene expression by increasing transcription rate and enhancing mRNA stability

Tong

Pitts

You

et al. 1998

Journal of Molecular Endocrinology

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“…The highest level of mRNA was observed in the hens exhibiting egg incubation behavior, whereas lowest levels were obtained from the non-photostimulated hens. The changes of expression of the PRL mRNA during reproductive cycles is well known (Talbot et al, 1991;Shimada et al, 1991;Wong et al, 1991;Kansaku et al, 1994) with lowest levels observed during non-egg laying states which increase during egg laying to reach maxima during incubation behavior. However, constant levels of Pit-1 mRNA expression throughout the reproductive cycle was reported in turkey (Wong et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…However, the levels of PRL mRNA induced by VIP injection or VIP perifusion are lower than those observed in incubating hen. Levels of PRL mRNA of incubating hen show a 10 to 50-fold increase compared to the laying hen (Wong et al, 1991;Kansaku et al, 1994), whereas, VIP induced increases of PRL mRNA show less than 5-fold increases (Talbot et al, 1991). Since levels of Pit-1 mRNA show constant during reproductive cycles (Wong et al, 1992), it is very difficult to attribute that VIP is only factor which affects levels of PRL mRNA during reproductive cycles.…”

Section: Introductionmentioning

confidence: 95%

Characterization of Chicken Prolactin Regulatory Element Binding Protein and its Expression in the Anterior Pituitary Gland during Embryogenesis and Different Reproductive Stages

et al. 2015

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The PRL regulatory element binding protein (PREB) is a transcription factor that specifically binds to a Pit-1 binding element in the PRL promoter to regulate PRL gene expression in mammals. However, it is unknown whether chicken PREB involves the expression of PRL mRNA or not. This study aimed to clone and characterize the chicken PREB gene and to investigate the mRNA expression during embryogenesis and different reproductive stages. Based on the conserved sequence of the human, rats and mouse, primers pairs were designed and applied to amplify the PREB cDNA fragment. After sequencing of PCR products, 5′ -and 3′ -end of mRNA were amplified and determined. To identify the structure of the PREB gene, PCR amplification was conducted. The chicken PREB gene consisted of 9 exons and 8 introns and encoded for a 411 amino acid protein. The expression of PREB mRNA in the anterior pituitary gland was measured during embryogenesis and at different reproductive stages. PREB mRNA was detectable at embryonic day 12. Significant increase of levels of PREB mRNA was detected at embryonic day 18 and a maximum level was detected at day 1 of chick. In adult Silkie hens, the lowest level of PREB mRNA was detected in non-photostimulated hens whereas the highest level was observed in incubating hens. Since both the expression of PREB mRNA and PRL mRNA show a similar profile, PREB might be involved in expression of PRL mRNA. An alternative-splicing isoform, which lacks exon 7, was also detected. Because the predicted translated protein originating from this splicing isoform lacked most of the region of WD3 repeat region, this isoform may either have no function or have an alternative function. The results of this study support the possibility of involvement of PREB in PRL mRNA expression in the chicken anterior pituitary gland.

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Changes in Levels of Immunoreactive Prolactin Isoforms during a Reproductive Cycle in Turkey Hens

Bédécarrats

Guemene²,

Kühnlein

et al. 1999

General and Comparative Endocrinology

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Cloning of a turkey prolactin cDNA: Expression of prolactin mRNA throughout the reproductive cycle of the domestic turkey (Meleagris gallopavo)

Cited by 68 publications

References 28 publications

Vasoactive intestinal peptide stimulates turkey prolactin gene expression by increasing transcription rate and enhancing mRNA stability

Vasoactive intestinal peptide stimulates turkey prolactin gene expression by increasing transcription rate and enhancing mRNA stability

Characterization of Chicken Prolactin Regulatory Element Binding Protein and its Expression in the Anterior Pituitary Gland during Embryogenesis and Different Reproductive Stages

Changes in Levels of Immunoreactive Prolactin Isoforms during a Reproductive Cycle in Turkey Hens

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