Cloning of Alu‐containing cDNAs from human fibroblasts and identification of small Alu⁺ poly(A)⁺ RNAs in a variety of human normal and tumor cells

Abstract

Two clones have been selected from a human fibroblast cDNA bank. By DNA sequencing the clones were shown to contain Alu elements located near the ends of the cDNA inserts. DNA of the clones was used for Northern blot hybridization analysis of a number of poly(A)-containing RNAs from normal human tissues (brain, stomach, uterus, spleen, fibroblasts) and tumors (neurinoma, glioma, neuroblastoma, liposarcoma, adrenal cortex adeno~rcinoma).All RNA samples reveal a heterodisperse distribution of Alu transcripts with discrete bands in the region of '7-12 S RNA. The majority of these smali poly(A)+ Alu+ RNAs contain Alu sequences only in one (canonical) orientation with functional signals including the split promoter for RNA polymerase III.