Cloning of antifungal gene from Bacillus licheniformis by application of low energy ion beam bombardment

Mahadtanapuk, S.; Sanguansermsri, M.; Lei, Yu; Vilaithong, T.; Anuntalabhochai, S.

doi:10.1016/j.surfcoat.2009.02.072

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…In order to determine the expression of the lipase gene in yeast, the entire lipase gene was subcloned into pYES2 and named pYES2-LicLip, then transferred into yeast cell via low-energy ion beam. The result showed that only the yeast transformed with pYES2-Liclip induced colonies and B. licheniformis induced colonies presented antifungal activity to plant pathogen, collectotrichum musae [18].…”

Section: Ion Beam Induction Of Mutationsmentioning

confidence: 99%

Low-energy Ion Beam Biology Research at Chiang Mai University

Anuntalabhochai

2011

Trans. Mat. Res. Soc. Japan

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$ Both authors contributed equallyIon beam at energy lower than tens of keV has been found to have significant biological effects on living materials. The finding has led to applications of ion-beam induced mutation and gene transfer. From the theoretical point of view, the low-energy ion beam effects on biology are difficult to understand with the knowledge of high-energy ion irradiation of biological matter since the ion range is so short that the ions can hardly directly interact with the key biological molecules for the changes. This paper introduces interesting aspects of low-energy ion beam biology, including basis of ion beam biotechnology and recent developments achieved in Chiang Mai University in relevant applications such as mutation and gene transfer and investigation on mechanisms involved in the low-energy ion interaction with biological matter such as eV-keV ion beam bombardments of naked DNA and the cell envelopes.

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Section: Ion Beam Induction Of Mutationsmentioning

confidence: 99%

Low-energy Ion Beam Biology Research at Chiang Mai University

Anuntalabhochai

2011

Trans. Mat. Res. Soc. Japan

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“…The result showed that only the yeast transformed with pYES2-Liclip induced colonies and B. licheniformis induced colonies presented antifungal activity to plant pathogen, collectotrichum musae [17]. The use of natural antagonists has recently been applied for biological control.…”

Section: Gene Cloningmentioning

confidence: 99%

Low-energy Ion Beam Biotechnology at Chiang Mai University

Anuntalabhochai

2009

MRS Proc.

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MeV-ion beam has long been applied to research and applications in biology for many decades as highly energetic ions are undoubtedly able to interact directly with biomolecules to cause biological changes. However, low-energy ion beam at tens of keV and even lower has also been found to have significant biological effects on living materials. The finding has led to applications of ion-beam induced mutation and gene transfer. From the theoretical point of view, the low-energy ion beam effects on biology are difficult to understand since the ion range is so short that the ions can hardly directly interact with the key biological molecules for the changes. This talk introduces interesting aspects of low-energy ion beam biology, including basis of ion beam biotechnology and recent developments achieved in Chiang Mai University in relevant applications such as mutation and gene transfer and investigations on mechanisms involved in the low-energy ion interaction with biological matter such as eV-keV ion beam bombardments of naked DNA and the cell envelopes.

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Combined quantum-mechanics/molecular-mechanics dynamics simulation of A-DNA double strands irradiated by ultra-low-energy carbon ions

Ngaojampa

Nimmanpipug

et al. 2011

Nuclear Instruments and Methods in Physics Research Section B:

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Cloning of antifungal gene from Bacillus licheniformis by application of low energy ion beam bombardment

Cited by 3 publications

References 24 publications

Low-energy Ion Beam Biology Research at Chiang Mai University

Low-energy Ion Beam Biology Research at Chiang Mai University

Low-energy Ion Beam Biotechnology at Chiang Mai University

Combined quantum-mechanics/molecular-mechanics dynamics simulation of A-DNA double strands irradiated by ultra-low-energy carbon ions

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