Cloning of B cell lymphoma-associated antigens using modified phage-displayed expression cDNA library and immunized patient sera

Chul, Soung; Kwak, Larry W.; Ruffini, Pier Adelchi; Qin, Hong; Neelapu, Sattva S.; Biragyn, Arya

doi:10.1016/j.jim.2006.02.013

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Surprisingly, we also find that healthy humans produce an auto-antibody against the Cterminal of NPIPB proteins ( Figure S18), confirming previous findings although the authors did not associate the target with NPIP (15). Recognition of self-antigens in human autoimmune diseases involves both T and B cell responses and may suggest a role for NPIP genes in immunity or autoimmunity.…”

Section: Discussionsupporting

confidence: 89%

“…In addition, immunohistochemistry analyses using MP2 antibodies show that the endogenous NPIPB protein localized primarily within the nucleus in HeLa cells (Figures 5, 6 and S17; see below). In a recent screen for B cell lymphoma-associated antigens, Cha and colleagues, unknowingly cloned four different constructs corresponding to a specific auto-antibody fraction of the isolated IgG purified from both treated and normal individuals (15). Our detailed analysis shows that, in fact, clones FL-aa-4-1, FL-aa-4-2, FL-aa-4-3, and FL-aa-4-4 correspond to the Cterminal repeat region of the NPIPB protein.…”

Section: Protein Analyses and Subcellular Localizationmentioning

confidence: 99%

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Functional Characterization of the Morpheus Gene Family

Bekpen

Baker

Hebert

et al. 2017

Preprint

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DATA ACCESSThe cDNA sequences reported in this paper have been deposited in the GenBank database (accession numbers): KF175165-KF175225 and BACs accession numbers that are used in this study: AC148621, AC190226, AC097327, AC097332, AC190226, AC097333, AC145401, AC187943, AC166855, AC166597, AC167295, AC235773, AC202644, and AC234805.ABSTRACTThe burst of segmental duplications during human and great ape evolution focuses on a set of “core” duplicons encoding great-ape-specific gene families. Characterization of these gene families is complicated by their high copy number, incomplete sequence, and polymorphic nature. We investigate the structure, transcriptional diversity, and protein localization of the nuclear pore complex-interacting protein (NPIP) or Morpheus gene family. The corresponding core, LCRA, encodes one of the most rapidly evolving genes in the human genome; LCRA has expanded to ~20 copies from a single ancestral locus in Old World monkey and is associated with most of the recurrent chromosome 16 microdeletions implicated in autism and mental retardation. Phylogenetic analysis and cDNA sequencing suggest two distinct subfamilies or subtypes, NPIPA and NPIPB. The latter expanded recently within the great apes due to a series of structural changes within the canonical gene structure. Among Old World monkey, we observe a testis-specific pattern of expression that contrasts with the ubiquitous pattern observed among human tissues. This change in the expression profile coincides with the structural events that reshaped the structure and organization of the gene family. Most of the expressed human copies are capable of producing an open reading frame. Immunofluorescence analyses of the morpheus genes showed a primary localization to both the nucleus and its periphery. We show that morpheus genes may be upregulated upon pI:C treatment and find evidence of human autoantibodies produced against the NPIPB protein, raising the possibility that morpheus genes may be related to immune- or autoimmune-related function.

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Section: Discussionsupporting

confidence: 89%

Section: Protein Analyses and Subcellular Localizationmentioning

confidence: 99%

Functional Characterization of the Morpheus Gene Family

Bekpen

Baker

Hebert

et al. 2017

Preprint

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“…This strategy allowed the identification of noncontinuous conformation-dependent epitope–protein sequences. 17,33 Whole-genome fragment phage display libraries have been used to elucidate different adjuvants’ effects on the antibody repertoire against an H5N1 vaccine in humans. 34,35 Moreover, the whole-genome phage display library was used for in-depth immune profiling of IgG and IgM antibody repertoires in longitudinal serum and urine samples from individuals acutely infected with the Zika virus.…”

Section: Filamentous Bacteriophage Display Peptidesmentioning

confidence: 99%

Filamentous bacteriophages, natural nanoparticles, for viral vaccine strategies

wang

Zhang²,

Zhong

et al. 2022

Nanoscale

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“…Among the most conspicuous spots recognized by plasma from CaP patients in VCaP lysates was spot 6; this hypoxia-sensitive spot was strongly reactive with the pooled sera of CaP patients ( Figure 6). This spot contains the U1 small nuclear ribonucleoprotein 70kDa and the heterogeneous nuclear ribonucleoprotein L. Both these proteins have been identified as TAAs (Cui, Li et al 2005;Cha, Kwak et al 2006;Li, Zhao et al 2006); their validation in CaP is our next immediate priority. For validation we will narrow the pH range of isoelectric focusing from 7 to 10 and use specific antibodies to confirm the identity of the molecules.…”

Section: Po 2 = 2kpamentioning

confidence: 99%

Enhancing Therapeutic Cellular Prostate Cancer Vaccines

Gómez¹

2013

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE June 2013 REPORT TYPE PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)University of Mississippi Medical Center, Jackson, MS, 39216 PERFORMING ORGANIZATION REPORT NUMBER SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel CommandFort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTES ABSTRACTScope: Prostate Cancer (CAP) is characterized by unique prostate-associated antigens; hence, it has been considered a prime candidate for immunotherapy. Despite numerous laboratory advances, clinical outcomes have been partial and transient. Purpose: The overall goal of the proposed studies is to optimize the effectiveness of therapeutic whole-cell CaP vaccines by taking into consideration tumor-associated hypoxia as a relevant determinant of tumor antigenicity. Major Findings: Gene expression in hypoxically cultured cells is more akin to that in tumor cells in situ than are cells grown normoxically. Transcripts of hypoxia-associated genes HURP/DLG7, CCNBI and HMMR were associated with Gleason score and with disease prognosis suggesting their potential as CaP biomarkers with prognostic value. By 2D-gel electrophoresis, we screened patient sera and detected novel hypoxic-cell reactive autoantibodies. Three potential TAAs; two hypoxia-specific (HSP70 and hnRNP L) and one hypoxia-nonspecific (HSP60) were selected for validation. The frequency of HSP60 autoantibodies was elevated in the CaP patient plasma; however the frequency of HSP70 autoantibodies was not elevated in CaP patients relative to healthy controls. Significance: Our data suggests that hypoxically cultured CaP cells are more akin to tumor sells in situ than are cells grown normoxically. We have identified hypoxia-reactive proteins, pathways and autoantigens with potential value as biomarkers or therapeutic targets. IntroductionProstate cancer (CaP) remains among the most common causes of cancer-related deaths in men. Because CaP is characterized by unique prost...

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Cloning of B cell lymphoma-associated antigens using modified phage-displayed expression cDNA library and immunized patient sera

Cited by 6 publications

References 24 publications

Functional Characterization of the Morpheus Gene Family

Functional Characterization of the Morpheus Gene Family

Filamentous bacteriophages, natural nanoparticles, for viral vaccine strategies

Enhancing Therapeutic Cellular Prostate Cancer Vaccines

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