Cloning of cDNAs and the differential expression of A‐type cyclins and Dmc1 during spermatogenesis in the Japanese eel, a teleost fish

Kajiura-Kobayashi, Hiroko; Kobayashi, Tohru; Nagahama, Yoshitaka

doi:10.1002/dvdy.20289

Cited by 23 publications

(23 citation statements)

References 32 publications

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“…The genes vasa and sox2 are expressed in spermatogonia and spermatocytes of Nile tilapia (Kobayashi et al 2002, Supplementary Figure 4, see section on supplementary data given at the end of this article) while dmc1 is expressed only in meiotic cells (Kajiura- Kobayashi et al 2005). In s-males the relative abundance of the analysed germ line-specific genes was higher than in d-males (Fig.…”

Section: Gene Expression Analysis Of Testes From Dominant and Subordimentioning

confidence: 90%

The social status of the male Nile tilapia (Oreochromis niloticus) influences testis structure and gene expression

Pfennig¹,

Kurth²,

Meißner³

et al. 2012

Reproduction

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Dominant and territorial behaviour are known social phenomena in cichlids and social stress influences reproduction and growth. The gonadotropic hormones trigger spermatogenesis and subordinate males have typically lower levels of gonadotropins than dominant males. In this study, we compared testis morphology and gene expression of dominant and subordinate Nile tilapia males (d-and s-males) in socially stable communities. The d-males had the highest gonadosomatic index but they were not the largest animals in the majority of studied cases. Long-term d-males showed large groups of Leydig cells and hyperplasia of the tunica albuginea due to numerous cytochrome-P450-11b-hydroxylase (Cyp11b) expressing myoid cells. Increased Cyp11b expression in d-males was reflected by elevated 11-ketotestosterone plasma values. However, immunofluorescence microscopy and expression analysis of selected genes revealed that most s-males conserved their capability for spermatogenesis and are, therefore, ready for reproduction when the social environment changes. Moreover, in s-males gene expression analysis by quantitative RT-PCR showed increased transcript levels for germ line-specific genes (vasa, sox2 and dmc1) and Sertoli-specific genes (amh, amhrII and dmrt1) whereas gene expression of key factors for steroid production (sf1 and cyp11b) were reduced. The Nile tilapia is a promising model to study social cues and gonadotropic signals on testis development in vertebrates.

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Section: Gene Expression Analysis Of Testes From Dominant and Subordimentioning

confidence: 90%

The social status of the male Nile tilapia (Oreochromis niloticus) influences testis structure and gene expression

Pfennig¹,

Kurth²,

Meißner³

et al. 2012

Reproduction

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show abstract

“…In that study, Dmc1 mRNA was abundantly expressed in the testes and ovaries, and lower expressed in the brain. In situ hybridization revealed that Dmc1 of A. japonica was localized only in primary spermatocytes, implying its important role during the initial stages of spermatogenesis (19).…”

Section: Rt-pcr and Tissue Distribution Analysismentioning

confidence: 99%

“…Recently, the full length Dmc1 cDNA was cloned from the testis of the Japanese eel (Anguilla japonica) (19). In that study, Dmc1 mRNA was abundantly expressed in the testes and ovaries, and lower expressed in the brain.…”

Section: Rt-pcr and Tissue Distribution Analysismentioning

confidence: 99%

Suppression subtractive hybridization (SSH) for isolation and characterization of genes related to testicular development in the giant tiger shrimp Penaeus monodon

Leelatanawit

Klinbunga²,

Aoki³

et al. 2008

BMB Reports

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“…The role of Dmc1 in promoting interactions between homologous chromosomes appears to be conserved from yeast to mammals (Tarsounas et al, 1999). On the other hand, our recent study indicates that the Dmc1 protein is expressed in meiotic prophase specifically during spermatogenesis in the teleost fish Anguilla japonica (Kajiura-Kobayashi et al, 2005).…”

Section: Introductionmentioning

confidence: 91%

“…To generate antibodies against Dmc1, we used as the immunizing antigen an oligopeptide that corresponds to the C-terminal amino acid sequence (tilapia Dmc1: AB18246, Kajiura-Kobayashi et al, 2005) predicted from tilapia Dmc1 cDNA, with the addition of a cysteine at the Nterminus to facilitate linkage to the carrier protein, as described previously (Kobayashi et al, 1996). Conjugation of the antigen to bovine serum albumin (BSA, fraction V; Sigma Chemical Co., St. Louis, MO) as the carrier protein was performed as described previously .…”

Section: Production Of the Anti-dmc1 Antibodymentioning

confidence: 99%

In vitro germ cell differentiation during sex differentiation in a teleost fish

Kobayashi¹

2010

Int. J. Dev. Biol.

Self Cite

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To clarify the sexually dimorphic mechanisms of gonadal sex differentiation, we established an in vitro culture system for gonadal sex differentiation using the teleost fish Oreochromis niloticus. In vivo, the entry of germ cells into meiosis occurs around 35 days after hatching (dah) in XX gonads, whereas in XY gonads, meiotic cells became differentiated around 85 dah. In our in vitro culture system using gonads from young fry at 23 dah, the meiotic cells in the XX gonads appeared after 21 days of culture. In contrast, in the XY gonads, no meiotic cells were detected after 21 days. These results indicate that germ cell differentiation in this culture system progresses in a manner similar to that in vivo. To identify the gene products that are involved in the entry of germ cells into meiosis or in the arrest of germ cells at the gonial stage of gonadal sex differentiation, we performed subtractive hybridization screening with this in vitro culture system. From the screening process, we identified the female-related gene, FR-3, which is a homolog of zebrafish nanos-related gene (nos). The nos gene was expressed after gonadal formation around 35 dah in XX gonads, but not in XY gonads. In situ hybridization indicated that nos is expressed in oogenic meiotic cells, but not in spermatogenic meiotic cells. Further examination revealed that nos was expressed in oogenic meiotic cells after gonadal formation, specifically in teleost fish. Together, nos may be also involved in oogenic meiosis, with the exception of primordial germ cell migration.

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Cloning of cDNAs and the differential expression of A‐type cyclins and Dmc1 during spermatogenesis in the Japanese eel, a teleost fish

Cited by 23 publications

References 32 publications

The social status of the male Nile tilapia (Oreochromis niloticus) influences testis structure and gene expression

The social status of the male Nile tilapia (Oreochromis niloticus) influences testis structure and gene expression

Suppression subtractive hybridization (SSH) for isolation and characterization of genes related to testicular development in the giant tiger shrimp Penaeus monodon

In vitro germ cell differentiation during sex differentiation in a teleost fish

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