Cloning of cDNAs encoding neurotoxic peptides from the spider Phoneutria nigriventer

Kalapothakis, Evanguedes; Penaforte, C.L.; Beirão, Paulo Sérgio Lacerda; Romano-Silva, Marco Aurélio; Cruz, Jáder Santos; Prado, Marco A. M.; Guimarães, Pedro Edson Moreira; Gomez, Marcus V.

doi:10.1016/s0041-0101(98)00105-6

Cited by 36 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were reported for components of other venoms, as described elsewhere, for toxins Pn2-1 and Tx2-1 of the spider Phoneutria nigriventer (Kalapothakis et al, 1998). The amino acids preceding the first residue of the mature PLA2 (FEEER) are similar to those of the propetides of the spider toxins (VEELER).…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus

Valdez‐Cruz

Segovia

Corona

et al. 2007

Gene

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 86%

“…The amino acids preceding the first residue of the mature PLA2 (FEEER) are similar to those of the propetides of the spider toxins (VEELER). They are rich in glutamic acids and the last residue is the basic arginine (Kalapothakis et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus

Valdez‐Cruz

Segovia

Corona

et al. 2007

Gene

View full text Add to dashboard Cite

“…Standard recombinant DNA techniques were carried out as described elsewhere [11]. Using the cDNA library and the methodology described previously [5], the cDNA encoding PnTx2‐6 was obtained. The clone was sequenced on both strands using the chain termination method [12] with an automated ALF DNA sequencer (Pharmacia).…”

Section: Methodsmentioning

confidence: 99%

“…Northern blots were carried out as described previously [5], using total RNA purified from the venom gland and from the spider body (without the glands) [14].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2‐6¹

et al. 2002

Self Cite

View full text Add to dashboard Cite

A cDNA with 403 nucleotides encoding the precursor of the toxin PnTx2-6 was cloned and sequenced. Subsequent analysis revealed that the precursor begins with a signal peptide and a glutamate-rich propeptide. The succeeding peptide con¢rmed the reported sequence of PnTx2-6. The puri¢ed toxin exerted complex e¡ects on Na + current of frog skeletal muscle. There was a marked decrease of the inactivation kinetics, and a shift to hyperpolarizing potentials of both the Na + conductance and the steady-state inactivation voltage dependences, along with a reduction of the current amplitude. The concentration dependence of the modi¢ed current suggests a K D of 0.8 W WM for the toxin^channel complex. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

show abstract

A novel strategy for the identification of toxinlike structures in spider venom

et al. 2005

View full text Add to dashboard Cite

We compared two different approaches to sequence information analysis from the expressed sequence tag (EST) library constructed for the venom glands of the spider Agelena orientalis. Some results were more illustrative and reliable by the contig analysis technique, whereas our novel method, with specific structural markers introduced for protein structure detection, allowed us to overcome some limitations of the contig analysis. A novel technique was suggested for the identification in data banks of the spider's ion channel inhibitor toxins using primary structure features common to all spiders. Analysis of about 150 polypeptides made it possible to introduce 3 primary structure motifs for spider toxins: the Principal Structural Motif (PSM), which postulates the existence of 6 amino acid residues between the first and second cysteine residue and the Cys-Cys sequence at a distance of 5-10 amino acid residues from the second cysteine; the Extra Structural Motif (ESM), which postulates the existence of a pair of CXC fragments in the C-region; and the Processing Quadruplet Motif (PQM), which specifies the Arg residue at position -1 and Glu residues at positions -2, -3, and/or -4 in the precursor sequences just before the postprocessing site. In the processed data bank we found 48 toxinlike structures with ion channel inhibitor motifs. These include agelenin earlier isolated from Agelena opulenta and 25 more homologous sequences, 15 homologs of mu-agatoxin 2 from the spider Agelenopsis aperta, 3 structures with low homology to omega-agatoxin-IIIA, and 4 new structures. Also we showed that toxinlike structures exceed two thirds of the overall database sequences.

show abstract

Cloning of cDNAs encoding neurotoxic peptides from the spider Phoneutria nigriventer

Cited by 36 publications

References 19 publications

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus

Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2‐6¹

A novel strategy for the identification of toxinlike structures in spider venom

Contact Info

Product

Resources

About

Cloning of cDNAs encoding neurotoxic peptides from the spider Phoneutria nigriventer

Cited by 36 publications

References 19 publications

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus

Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2‐61

A novel strategy for the identification of toxinlike structures in spider venom

Contact Info

Product

Resources

About

Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2‐6¹