Cloning of crustacean cardioactive peptide and its functional verification under low-salt adaptation in swimming crab ( 
            <i>Portunus trituberculatus</i> )

Sun, Dongfang; Lyu, Jianjian; Gao, Bingbo; Huan, Pengpeng; Cai, Ying; Liu, Ping

doi:10.3724/sp.j.1118.2019.18207

Cited by 2 publications

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“…It is widely distributed in the estuary and coastal waters of Korea, Japan, China, and Southeast Asia ( Meng et al., 2020 ). The only neuropeptides reported in this species include CHH ( Xie et al., 2014 ), MIH ( Wang et al., 2013 ), and CCAP ( Sun et al., 2019 ). In addition, no studies have reported the P. trituberculatus neuropeptidome.…”

Section: Introductionmentioning

confidence: 86%

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

Wang

et al. 2021

PeerJ

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Neuropeptides and their G protein-coupled receptors (GPCRs) regulate multiple physiological processes. Currently, little is known about the identity of native neuropeptides and their receptors in Portunus trituberculatus. This study employed RNA-sequencing and reverse transcription-polymerase chain reaction (RT-PCR) techniques to identify neuropeptides and their receptors that might be involved in regulation of reproductive processes of P. trituberculatus. In the central nervous system transcriptome data, 47 neuropeptide transcripts were identified. In further analyses, the tissue expression profile of 32 putative neuropeptide-encoding transcripts was estimated. Results showed that the 32 transcripts were expressed in the central nervous system and 23 of them were expressed in the ovary. A total of 47 GPCR-encoding transcripts belonging to two classes were identified, including 39 encoding GPCR-A family and eight encoding GPCR-B family. In addition, we assessed the tissue expression profile of 33 GPCRs (27 GPCR-As and six GPCR-Bs) transcripts. These GPCRs were found to be widely expressed in different tissues. Similar to the expression profiles of neuropeptides, 20 of these putative GPCR-encoding transcripts were also detected in the ovary. This is the first study to establish the identify of neuropeptides and their GPCRs in P. trituberculatus, and provide information for further investigations into the effect of neuropeptides on the physiology and behavior of decapod crustaceans.

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Section: Introductionmentioning

confidence: 86%