Cloning of cytoplasmic heat shock protein 90 (FcHSP90) from Fenneropenaeus chinensis and its expression response to heat shock and hypoxia

Abstract

Heat shock protein 90 (HSP90) works as a multifunctional chaperone and is involved in the regulation of many essential cellular pathways. In this study, we have identified a full-length complementary DNA (cDNA) of HSP90 (FcHSP90) from Chinese shrimp Fenneropenaeus chinensis. FcHSP90 full-length cDNA comprised 2,552 bp, including a 2,181-bp open reading frame encoding 726 amino acids. Both homology analyses using alignment with previously identified HSP90 and a phylogeny tree indicated that FcHSP90 was a cytoplasmic HSP90. Real-time reverse transcription polymerase chain reaction analysis revealed that FcHSP90 was ubiquitously expressed in all the examined tissues but with highest levels in ovary of F. chinensis. FcHSP90 mRNA levels were sensitively induced by heat shock (from 25°C to 35°C) and reached the maximum at 6 h during heat shock treatment. Under hypoxia conditions, FcHSP90 mRNA levels, in both hemocytes and gill, were induced at 2 h and depressed at 8 h during hypoxia stress. The assessment of FcHSP90 mRNA levels under heat shock and hypoxia stresses indicated that the transcription of FcHSP90 was very sensitive to heat shock and hypoxia, so we deduced that FcHSP90 might play very important roles for shrimp to cope with environmental stress.

Expression of hsp90 mediates cytoprotective effects in the gastrodermis of planarians

“…Scale bar 400 μm hsp90 in planarianssituations. The transcriptional up-regulation of Djhsp90 is consistent with that observed in a variety of organisms after exposition to different stressors (Farcy et al 2007;Gao et al 2007;Lei et al 2009;Li et al 2009a;Manchado et al 2008;Tokalov et al 2007;Zhang et al 2009) and indicates that this gene encodes an inducible HSP90 form. To assess the function of Djhsp90 in intestinal cells, we used RNA interference (RNAi).…”

“…Indeed, gastrodermal cells are short-lived cells (Baguñà and Romero 1981) and are continuously being replaced by differentiating neoblasts, as have been extensively documented by light and electron microscopy (Hori 1986). HSP90 is one of the most abundant proteins in the cytosol of eukaryotic cells even in the absence of stress, being required for final maturation of different substrates (Csermely et al 1998;Nathan et al 1997), and its distribution pattern (ubiquitary or tissue-specific) varies in different organisms (Krone and Sass 1994;Li et al 2009a, b). The constitutive hsp90 expression pattern, detected in D. japonica and also conserved in S. mediterranea (data not shown), seems to be a typical feature of planarians.…”

Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses

“…Based on the present data, we inferred that FcHsp70 played more crucial roles in protecting the cells from damage than FcHsc70 during heat shock treatment, while FcHsc70 may be more important in protecting the cells when shrimp were transferred from high-to low-temperature water. In our previous study, the data also showed that the expression of HSP90 of Chinese shrimp (FcHsp90) was dramatically upregulated and kept at high levels during the heat shock treatment and became down-regulated after the shrimp were returned to water at 25°C from 36°C (Li et al 2009). When considering another HSP70 family member Grp78 of F. chinensis, we found that its expression level increased gradually during heat shock treatment, then returned to the same level as that of controls after the shrimp were returned back to water at 25°C (Luan et al 2007).…”

“…The procedures and sampling methods for culturing the juvenile shrimp during heat shock treatment are the same as those described previously (Li et al 2009). Briefly, six shrimp were randomly collected at 1, 2, 3, 4, and 6 h, respectively, during heat shock treatment at 35±1°C.…”

Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

“…Hence, the HSP90 family provides a useful model system for evolutionary studies (Gupta 1995). In addition, the response of these proteins to environmental stresses, such as changes in temperature (Palmisano et al 2000;Hermesz et al 2001;Landais et al 2001), hyperosmotic shock (Pan et al 2000), hypoxia (Li et al 2008), metal ions (Ali et al 1996), polychlorinated biphenyls (Wiens et al 2000), and also diseases (Ramaglia et al 2004) has been examined. Despite of these various studies, only five complete cDNA sequences have been reported from mollusks (Farcy et al 2007;Gunter and Degnan 2007), including three HSP90 cDNA sequences in bivalves (Gao et al 2007(Gao et al , 2008Choi et al 2008).…”