Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics

Tian, Wen; Gao, Yue‐Dong; Guo, Yan; Huang, Jing‐Fei; Xiao, Chang; Li, Zuosheng; Zhang, Hua-Tang

doi:10.3724/sp.j.1141.2012.01060

Cited by 2 publications

(4 citation statements)

References 16 publications

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“…Whilst similar, differences in negative charges and N-glycosylation sites in the CD4 extracellular domain D1, thought to contain the antibody binding site, are evident between human and tupaia (19). Therefore, there is no cross-species reactivity of mouse anti-human CD4 antibody to tCD4 (19). Similarly, the potential glycosylation sites in tupaia CD3ε (tCD3ε) differ from human CD3ε and surface charges in the extracellular domain are also different, meaning antibodies against human or mouse CD3ε are not reactive against tCD3ε (20).…”

Section: Lymphocytesmentioning

confidence: 95%

“…Two important T lymphocyte differentiation molecules, CD4 and CD3ε, have been identified in the tupaia. Tian et al cloned the full-length coding sequence of the tupaia CD4 (tCD4) and demonstrated that the tCD4 amino acid sequence shared high similarity to human and macaque (19). Whilst similar, differences in negative charges and N-glycosylation sites in the CD4 extracellular domain D1, thought to contain the antibody binding site, are evident between human and tupaia (19).…”

Section: Lymphocytesmentioning

confidence: 99%

“…Tian et al cloned the full-length coding sequence of the tupaia CD4 (tCD4) and demonstrated that the tCD4 amino acid sequence shared high similarity to human and macaque (19). Whilst similar, differences in negative charges and N-glycosylation sites in the CD4 extracellular domain D1, thought to contain the antibody binding site, are evident between human and tupaia (19). Therefore, there is no cross-species reactivity of mouse anti-human CD4 antibody to tCD4 (19).…”

Section: Lymphocytesmentioning

confidence: 99%

“…Similarly, the potential glycosylation sites in tupaia CD3ε (tCD3ε) differ from human CD3ε and surface charges in the extracellular domain are also different, meaning antibodies against human or mouse CD3ε are not reactive against tCD3ε (20). In the cytoplasmic domain, the tyrosine protein kinase-binding residues (KKTCQC) in CD4 play a pivotal role in TCR-mediated signal transduction and activation (19). This region is conserved between tupaias and humans.…”

Section: Lymphocytesmentioning

confidence: 99%

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The tree shrew as a model for infectious diseases research

Li¹,

Zanin²,

Xia³

et al. 2018

J. Thorac. Dis.

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Despite major advances in medicine, infectious diseases still pose a significant threat to humanity. Mammalian models of disease have proved extremely useful in adding to the understanding of infectious diseases and the development of prophylactic and/or therapeutic interventions. Arguably the most important considerations of any animal model are (I) the similarity of the model to humans with respect to anatomy, physiology, immunology and disease progression, and (II) the expense of conducting experiments using the model organism. Often the choice of a model represents a compromise between these factors. Here we review the Northern Tree shrew (), or tupaia, as a useful model for the study of infectious diseases. Tupaias are non-human primates similar in size to squirrels that are indigenous to Asia. Their genome has been sequenced and, overall, shows relatively high similarity to humans. There is also a close homology of many aspects of tupaia biology with human biology. Importantly, from an infectious diseases viewpoint, tupaias are susceptible to infection with unadapted human pathogens and manifest clinical signs akin to human infections. Overall, the relatively small size of the tupaia, their homology to humans and their susceptibility to human pathogens make them a useful model for the study of infectious diseases.

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Section: Lymphocytesmentioning

confidence: 95%

Section: Lymphocytesmentioning

confidence: 99%