Cloning of <i>aas</i>, a gene encoding a <i>Staphylococcus saprophyticus</i> surface protein with adhesive and autolytic properties

Hell, Wolfgang; Meyer, Heinz‐Georg W.; Gatermann, Sören

doi:10.1046/j.1365-2958.1998.00983.x

Cited by 138 publications

(163 citation statements)

References 42 publications

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“…There is increasing evidence that staphylococcal autolysins are important in pathogenesis (Hell et al, 1998;Mani et al, 1994;Rupp et al, 1998). Three similar genes in S. aweits, S. epidermidis and S. saprophyticus en c od i n g ce 1 1 -w a 11 -ass o c i at ed auto 1 y s i n s have been sequenced: at1 (Oshida et al, 1995(Oshida et al, , 1998, at1E and aas (Hell et al, 1998), respectively.…”

Section: Adhesion Factors and Genes In S Capraementioning

confidence: 99%

“…Three similar genes in S. aweits, S. epidermidis and S. saprophyticus en c od i n g ce 1 1 -w a 11 -ass o c i at ed auto 1 y s i n s have been sequenced: at1 (Oshida et al, 1995(Oshida et al, , 1998, at1E and aas (Hell et al, 1998), respectively. T h e three proteins encoded by these genes have two enzymic domains (amidase and glucosaminidase) in common.…”

Section: Adhesion Factors and Genes In S Capraementioning

confidence: 99%

“…The routine use of molecular methods to identify staphylococci at the species level (Chesneau et al, 1992(Chesneau et al, , 1993De Buyser et af., 1989 led to the detection, in our reference centre, of 10 S. caprae strains from four patients suffering from bone and joint infections following the implantation of orthopaedic material. The aim of this study was to characterize these ten strains and four unrelated S. caprae strains isolated from the milk of mastitis-free goats and, in particular, to track the adhesion factors or genes that have been shown to be implicated in the pathogenesis of staphylococci (Bedidi-Madani et al, 1998a;Christensen et al, 1983 ;Deighton et al, 1996 ;Greene et al, 1995 ;Heinz et a/., 1996;Hell et al, 1998;Moreillon et al, 1995;Patti et al, 1994;Rupp et al, 1998;Vaudaux et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…4, lane 5) was sequenced. T h e amino acid sequence obtained was AEETQDQTKN, which is 80% identical to the Nterminal part of S. aurezts At1 ( A E T T Q D Q T T N ) (Oshida et al, 1995), 7 0 % identical to the N-terminal part of Staphvlococczts saprophvticus Aas (ASNT-Q D Q T P N ) (Hell et al, 1998) and 50% identical to the same part of S. epidermidis AtlE (AEQPQNQSHN) (Heilmann et a/., 1997).…”

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confidence: 99%

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Tracking adhesion factors in Staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material

et al. 1999

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Ten Staphylococcus caprae strains isolated from four patients and responsible for bone infections following implantation of orthopaedic material were compared to four 5. caprae strains collected from milk samples of healthy goats. The following characteristics were investigated : SmaI patterns, hybridization patterns with pBA2 (ribotypes), slime production, adhesion to matrix proteins (fibrinogen, fibronectin, collagen) and the staphylococcal adhesion genes (fnbA, clfA, cna, at/€, ica, fbe). None of the characteristics enabled us to distinguish the human strains from the goat strains. Slime was occasionally produced by 5. caprae strains but all of them carried nucleotide sequences hybridizing at low stringency with the following genes: at/€ encoding a 5. epidermidis autolysin binding vitronectin and responsible for the primary adhesion to polystyrene, ica operon involved in the biosynthesis of a S. epidermidis extracellular polysaccharide, and the part of c/fA encoding the serine-aspartate repeated region of a 5. aureus cell-wall fibrinogen-binding protein.

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Section: Adhesion Factors and Genes In S Capraementioning

confidence: 99%

Section: Adhesion Factors and Genes In S Capraementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Tracking adhesion factors in Staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material

et al. 1999

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“…An examination of the data set produced by Sanger sequencing of metagenomic DNA from the Tammar wallaby microbiome (Pope et al, 2010) identified 20 additional muramidase genes, including several encoded on contigs with a high degree of sequence similarity and gene organization when compared with the sequenced fosmids (Figure 4a). Furthermore, a class of bifunctional autolysins found in a number of Staphylococci and implicated in cell adhesion and biofilm formation have been shown to possess both N-acetylmuramoyl-L-alanine amidase and endo-b-Nacetylglucosaminidase catalytic modules (Hell et al, 1998;Allignet et al, 2001;Heilmann et al, 2007;Qin et al, 2007) (Figure 4c). Our searches of the GenBank databases also showed that a 'hypothetical protein' present in the genomes of the Firmicutes-affiliated human gut bacteria Anaerostipes caccae DSM 14662 and Ruminococcus lactaris ATCC 29176 possess the same modular architecture (Figure 4b).…”

Section: Resultsmentioning

confidence: 99%

Muramidases found in the foregut microbiome of the Tammar wallaby can direct cell aggregation and biofilm formation

et al. 2010

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We describe here the role of muramidases present in clones of metagenomic DNA that result in cell aggregation and biofilm formation by Escherichia coli. The metagenomic clones were obtained from uncultured Lachnospiraceae-affiliated bacteria resident in the foregut microbiome of the Tammar wallaby. One of these fosmid clones (p49C2) was chosen for more detailed studies and a variety of genetic methods were used to delimit the region responsible for the phenotype to an open reading frame of 1425 bp. Comparative sequence analysis with other fosmid clones giving rise to the same phenotype revealed the presence of muramidase homologues with the same modular composition. Phylogenetic analysis of the fosmid sequence data assigned these fosmid inserts to recently identified, but uncultured, phylogroups of Lachnospiraceae believed to be numerically dominant in the foregut microbiome of the Tammar wallaby. The muramidase is a modular protein containing putative N-acetylmuramoyl-L-alanine amidase and an endo-b-N-acetylglucosaminidase catalytic module, with a similar organization and functional properties to some Staphylococcal autolysins that also confer adhesive properties and biofilm formation. We also show here that the cloned muramidases result in the production of extracellular DNA, which appears to be the key for biofilm formation and autoaggregation. Collectively, these findings suggest that biofilm formation and cell aggregation in gut microbiomes might occur via the concerted action of carbohydrate-active enzymes and the production of extracellular DNA to serve as a biofilm scaffold.

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Bacterial Infections of the Urinary Tract

Gatermann

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Cloning of aas, a gene encoding a Staphylococcus saprophyticus surface protein with adhesive and autolytic properties

Cited by 138 publications

References 42 publications

Tracking adhesion factors in Staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material

Tracking adhesion factors in Staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material

Muramidases found in the foregut microbiome of the Tammar wallaby can direct cell aggregation and biofilm formation

Bacterial Infections of the Urinary Tract

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