Cloning of
            <i>Salmonella enterica</i>
            Serovar Enteritidis Fimbrial Protein SefA as a Surface Protein in
            <i>Escherichia coli</i>
            Confers the Ability To Attach to Eukaryotic Cell Lines

Rank, Douglas; Saeed, Mahdi; Muriana, Peter M.

doi:10.1128/aem.00639-09

Cited by 8 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7b). Adhesion phenotype mediated by fimbrial genes expressed in E. coli strains was observed for other Salmonella fimbriae, such as Stg and Sef (Forest et al, 2007;Rank et al, 2009), confirming that fimbriae can contribute to the interaction with host cells.…”

Section: Discussionmentioning

confidence: 74%

Regulation and production of Tcf, a cable-like fimbriae from Salmonella enterica serovar Typhi

et al. 2016

View full text Add to dashboard Cite

boulevard des Prairies, Laval, Qué bec H7V 1B7, Canada tcf (Typhi colonization factor) is one of the 12 putative chaperone/usher fimbrial clusters present in the Salmonella enterica serovar Typhi genome. We investigated the production, expression and regulation of tcf as well as its role during interaction with human cells. The tcf gene cluster was cloned and induced in Escherichia coli and S. Typhi, and the production of intertwined fibres similar to the Cbl (cable) pili of Burkholderia cepacia was observed on the bacterial surface by electron microscopy. In S. Typhi, tcf was expressed more after growth in M63 minimal medium than in standard Luria-Bertani medium. Analysis of the promoter region identified putative binding sites for the global regulators RcsB, ArgR and Fur. The expression of tcf was measured in isogenic strains lacking these global regulators. Under the conditions tested, the results showed that tcf expression was higher in the fur mutant and was regulated by iron concentration. Fur may regulate these fimbriae indirectly via the small RNAs RyhB1 and RyhB2. An isogenic mutant harbouring a deletion of the tcf cluster did not demonstrate any defect in adhesion or invasion of human epithelial cells, or in phagocytosis or survival in macrophages, when compared to the WT serovar Typhi strain. However, the tcf cluster contributed to adherence to human epithelial cells when introduced into E. coli. Thus, tcf genes encode functional fimbriae that can act as an adhesin and may contribute to colonization during typhoid fever.

show abstract

Section: Discussionmentioning

confidence: 74%

Regulation and production of Tcf, a cable-like fimbriae from Salmonella enterica serovar Typhi

et al. 2016

View full text Add to dashboard Cite

show abstract

“…It was suggested that gene loss may be a mechanism of targeting the invading pathogen preferentially to particular tissues or host cells, and can also influence the ability of the pathogen to survive in the external environment or even in stressful situations within the host (Thomson et al, 2008;Feng et al, 2013). The enhanced invasion of the reproductive tract and survival in the forming egg of S. Enteritidis and S. Gallinarum has been linked to the presence of SEF-14 fimbriae ( (Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009). S. Enteritidis can colonize the intestinal epithelium of chickens and is then able to reach a variety of internal organ sites, notably the ovaries (Gast and Beard, 1990;Gast, 1994;Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009;Berghaus et al, 2011).…”

Section: Factors Influencing Salmonella Pathogenesis In Chickensmentioning

confidence: 99%

“…The enhanced invasion of the reproductive tract and survival in the forming egg of S. Enteritidis and S. Gallinarum has been linked to the presence of SEF-14 fimbriae ( (Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009). S. Enteritidis can colonize the intestinal epithelium of chickens and is then able to reach a variety of internal organ sites, notably the ovaries (Gast and Beard, 1990;Gast, 1994;Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009;Berghaus et al, 2011). Studies have shown that some S. Enteritidis phage types (PTs) are more invasive than others (Hinton et al, 1990;Roy et al, 2001), but other similarly designed studies could not identify any difference in the invasiveness or colonisation ability between different phage types (Barrow, 1991).…”

Section: Factors Influencing Salmonella Pathogenesis In Chickensmentioning

confidence: 99%

Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

2014

EFSA Journal

View full text Add to dashboard Cite

Salmonella Enteritidis is considered the only pathogen currently posing a major risk of egg-borne diseases in the European Union (EU). The possible impact of extending the shelf-life of eggs on the risk to consumers posed by S. Enteritidis was estimated by applying a quantitative model and comparing the actual situation regarding the storage of eggs in the EU with different possible scenarios combined, considering the prolongation of the bestbefore and the sell-by date from 7 to a maximum of 70 days. Extending the sell-by date by one week (from 21 to 28 days), but leaving the best-before date unchanged, is estimated to result in a relative risk of illness of 1.4 and 1.5 for uncooked and lightly cooked egg meals respectively, compared to the current situation. If the best-before date is also extended by one week (from 28 to 35 days), the relative risk would be 1.6 and 1.7. In the worst case scenario considered (sell-by date of 42 days, best before date of 70 days), such figures would be 2.9 and 3.5. It should be noted that the absolute risk is greater for uncooked meals compared to lightly cooked meals. An effective way to minimise any increase in risk during extended storage is to keep the eggs refrigerated both at retail and the household. Regarding egg spoilage, such events strongly depend on the hygienic conditions of egg production and practices of egg handling, including storage times and temperatures. Finally, the impact of the prolongation of storage time on the quality criteria for eggs (3-hydroxybutyric acid and lactic acid) destined for manufacturing of egg products is considered negligible. Specifically, EFSA was asked to assess: (i) the public health risk posed by relevant pathogens and in particular by Salmonella in the consumption and handling of table eggs (Term of Reference, or ToR1), (ii) the public health risk deriving from deterioration (ToR2) and (iii) possible consequences for public health of an extended shelf-life of table eggs for the specific freshness criteria for egg products as laid out in the hygiene package (ToR3). © European Food Safety Authority, 2014 KEY WORDSIn order to answer the first ToR, the BIOHAZ Panel based its conclusions on the results of a quantitative model, aimed at describing the behaviour of Salmonella Enteritidis following vertical transmission, since this serovar is recognised to be the major pathogen related to egg-borne disease because of its ability to contaminate the interior of intact eggs during their formation within the body of an infected hen. The possible impact of extending the shelf-life of eggs on other serovars of Salmonella, as well as on other pathogens, is evaluated in a qualitative manner, with no individual serovar other than S. Enteritidis currently posing a major risk of egg-borne salmonellosis in the European Union (EU), although S. Typhimurium has been associated with relatively small outbreaks due to duck eggs. Trans-shell contamination (i.e. secondary contamination) is also discussed, but the impact of a prolongation of the shelf ...

show abstract

“…S. Enteritidis also has the ability to alter the cell surface dramatically; it frequently produces glycosylated high-molecularmass LPS that may be important for persistence in eggs and may assist the efficiency of transmission of infection to man (Jean Guard-Petter, 2001). S. Enteritidis fimbrial protein (SefA) is also involved in the superior ability of S. Enteritidis to attach to ovarian granulosa cells and other cells (Rank et al, 2009). All these findings suggest that S. Enteritidis has a higher ability to colonise the reproductive epithelium than other serovars, and that the Salmonella LPS type may play an essential role in tropism of the reproductive tract.…”

Section: B Salmonella Contamination Of the Egg Contentsmentioning

confidence: 99%

Scientific Opinion on a quantitative estimation of the public health impact of setting a new target for the reduction of Salmonella in laying hens

Andréoletti¹,

Budka²,

Bunčić³

et al. 2010

EFS2

View full text Add to dashboard Cite

European Food Safety Authority (EFSA), Parma, Italy ABSTRACTPublic health risks of Salmonella infection in laying hens (Gallus gallus) can be associated with exposure through four different pathways: internally contaminated table eggs, externally contaminated table eggs, egg products and meat from spent hens. In relation to eggs, Salmonella Enteritidis is by far the serovar most frequently associated with human illness, and exposure through eggs that are internally contaminated with this serovar has a higher public health significance than exposure to externally contaminated eggs. A mathematical model, using reported field data from two EU Member States (MSs), suggests a linear relationship between the investigated scenarios of flock prevalence for Salmonella Enteritidis and the number of contaminated eggs that would be laid. However, the absolute public health impact of the assessed flock prevalence scenarios is highly uncertain due to lack of data on the number of contaminated eggs produced by infected flocks and on the true number of egg-related human salmonellosis cases. It is suggested that public health benefits, similar to those obtained reaching lower Salmonella flock prevalences, may be achieved by implementing controls based on more sensitive sampling protocols. Diversion of eggs from flocks that are tested positive in the EU Salmonella control programme to the production of egg products subjected to heat treatment may lead to increased health risks as heat treatment of egg products should not be considered an absolute barrier to Salmonella contamination. Fresh meat from spent laying hens might carry a higher prevalence of Salmonella than meat from broiler flocks, in particular if sourced from Salmonella-positive flocks. The quantification of under-ascertainment and underreporting of human salmonellosis cases, improving knowledge on within-flock dynamics of Salmonella and harvesting data on production of Salmonella contaminated eggs under field conditions would contribute to improving the accuracy of future quantitative estimates. KEY WORDSSalmonella, microbiological target, laying hen, eggs, quantitative microbiological risk assessment. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on a quantitative estimate of the public health impact of setting a new target for the reduction of Salmonella in laying hens. In particular, EFSA was asked to assess the relative public health impact if a new target for reduction of Salmonella is set for laying hen flocks being 1% or less remaining positive for all Salmonella serovars with public health significance, compared to (1) a theoretical prevalence of 2% of flocks remaining positive for Salmonella Enteritidis or Salmonella Typhimurium, and (2) the real prevalence in 2008 to be reported by the Member States (MSs).For this task, the BIOHAZ Panel took into account data from the first year of the harmonised EUwide monitoring and control programme of Salmonella in laying hens...

show abstract

Cloning of Salmonella enterica Serovar Enteritidis Fimbrial Protein SefA as a Surface Protein in Escherichia coli Confers the Ability To Attach to Eukaryotic Cell Lines

Cited by 8 publications

References 31 publications

Regulation and production of Tcf, a cable-like fimbriae from Salmonella enterica serovar Typhi

Regulation and production of Tcf, a cable-like fimbriae from Salmonella enterica serovar Typhi

Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

Scientific Opinion on a quantitative estimation of the public health impact of setting a new target for the reduction of Salmonella in laying hens

Contact Info

Product

Resources

About