Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.

Woude, George F. Vande; Oskarsson, M; Enquist, Lynn W.; Nomura, Shigeko; Sullivan, M.; Fischinger, Peter J.

doi:10.1073/pnas.76.9.4464

Cited by 100 publications

(54 citation statements)

References 33 publications

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“…Phage DNAs were prepared by methods described by Yamamoto et al (40) or Vande Woude et al (37). Methods for isolation of C. elegans DNA, restriction enzyme digests, agarose gel electrophoresis, and Southern blotting of DNA fragments were those described by Emmons,Klass,and Hirsh (17) or Davis, Botstein, and Roth (15).…”

Section: Methodsmentioning

confidence: 99%

Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans.

Cox¹,

Hirsh²

1985

Mol. Cell. Biol.

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Coliagens are the major protein components of the Caenorhabditis elegans cuticle and are encoded by a large family of 40 to 150 closely related but nonidentical genes. We have determined temporal patterns of mRNA accumulation for a large number of collagen genes by screening recombinant phages and plasmids containing cloned collagen genes under high stringency conditions with 32P-labeled cDNA preparations specific for eggs or three postembryonic molts. We find that collagen mRNA levels are regulated both temporally and quantitatively during C. elegans development. Most genes studied exhibit one of four patterns of mRNA accumulation which correlate with changes in cuticle morphology and collagen protein composition during development. Our results suggest that, in general, there is a progressive activation of new collagen genes during normal development.Multigene families appear to be ubiquitous components of metazoan genomes. In many gene families, individual family members differ in DNA and protein sequence and are expressed differentially, either -in temporal sequence (e.g., chorion genes [34]) or in tissue distribution (e.g., actin genes [21]). Because of these properties, multigene families are particularly well suited for studies of gene evolution and for analysis of factors controlling gene expression in eucaryotes.Collagens are fibrous structural proteins that are encoded by a multigene family in all organisms in which they have been identified (1, 18). All collagens share a characteristic amino acid sequence which can be represented as (Gly-X-Y), where X and Y can be any amino acids but are often proline or hydroxyproline. Collagens have been extensively studied in vertebrates, for which at least nine collagen types with different protein sequences and tissue distributions have been described (3,5). For the past few years, we have been studying the collagen gene family of the soil nematode Caenorhabditis elegans. Collagens constitute the major component of the extracellular cuticle of the nematode, which is sloughed off and reformed at each of four postembryonic molts under the direction of an underlying layer of hypodermal tissue, which, for the most part, consists of a single large syncytium that extends throughout the length of the animal (J. G. White, Ph.D. thesis, University of Cambridge, Cambridge, England, 1974). The cuticles formed at the different molts vary considerably in morphology and collagen protein composition, implying a complex pattern of collagen gene expression during C. elegans development (14). Determination of temporal programs of expression for specific collagen genes should aid in understanding the physiological roles of the different collagen proteins in the nematode and provide insights into the mechanisms controlling their differential expression. The information gained from these studies should be applicable to collagen gene families in other organisms.In previous reports, we showed that the collagen gene family in C. elegans is unusually large, consisting of 40 to 150 distinc...

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Section: Methodsmentioning

confidence: 99%

Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans.

Cox¹,

Hirsh²

1985

Mol. Cell. Biol.

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“…During the past several years, a number of different isolates of Mo-MuSV have been obtained and molecularly cloned (Vande Woude et al, 1979;Verma et aL, 1980). The only virus isolate known to induce a blood disease in vivo while retaining its sarcomatogenous activity in newborn mice is the myeloproliferative sarcoma virus (MPSV) (Le Bousse- .…”

Section: Introductionmentioning

confidence: 99%

Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. II. Haematopoietic Tropism and Tumourigenicity

Bousse-Kerdilès

Duménil

Smadja-Joffe

et al. 1985

Journal of General Virology

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“…Each strain displays a different set of deletions in the gag and pol regions (Donoghue et al, 1979). Four of them, namely HT 1, m 1, 124 MuSV and myeloproliferative sarcoma virus (MPSV), have been molecularly cloned (Vande Woude et al, 1979;Blair et al, 1980;Verma et al, 1980;Kollek et al, 1984) and extensively characterized in terms of structural organization and transforming potentialities.…”

Section: Introductionmentioning

confidence: 99%

Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. I. Molecular Cloning and Characterization

Devaux

Jouanneau

Quibriac

et al. 1985

Journal of General Virology

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SUMMARYThe 78A1 isolate of Moloney murine sarcoma virus (78A1 Mo-MuSV) was cloned from a genomic library obtained from virus producer rat cells, in the lambda vector L47. Among the recombinants hybridizing with a probe specific for the v-mos sequences, we recovered a recombinant which contained leukaemia virus (MuLV) sequences and was able to transform both mouse and rat cells in transfection experiments. The cloned provirus could be rescued by both Mo-MuLV ecotropic and amphotropic viruses in mouse cells, but only with the amphotropic helper virus in rat cells. Comparative restriction mapping indicates that the 78Al provirus is 200 bp longer than the HTI provirus. The difference lies in the gag-poljunction region of MoMuSV. Other minor differences were found in the gag region, whereas the restriction patterns of the Y parts of the proviruses were identical.

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Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.

Cited by 100 publications

References 33 publications

Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans.

Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans.

Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. II. Haematopoietic Tropism and Tumourigenicity

Study of the 78A1 Isolate of Moloney Murine Sarcoma Virus. I. Molecular Cloning and Characterization

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