Cloning of nitrite reductase gene from Haematococcus pluvialis and transcription and enzymatic activity analysis at different nitrate and phosphorus concentration

Shi, Jing; Zang, Xiaonan; Cong, Xiao; Hou, Lulu; He, Bian; Ding, Yating; Dong, Manman; Sun, Deguang; Guo, Yalin; Feng, Zhang; Wang, Zhendong; Wei, Xuehong; Zhang, Xuecheng

doi:10.1016/j.gene.2019.01.042

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…The nitrogen metabolism pathway indicates that functional denitrification genes such as nirS (playing an important role in nitrite reduction), norB , nosZ , and narG [ 72 ] are detected in P. stutzeri with denitrification. In addition, nitrate is metabolized into ammonia through dissimilatory nitrate reduction, and the annotated gene nirBD encodes nitrite reductase (EC:1.7.1.15) and can contribute to nitrite reduction [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

et al. 2022

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Background Pseudomonas stutzeri S116 is a sulfur-oxidizing bacteria isolated from marine sludge. It exhibited excellent electricity generation as bioanode and biocathode applied in microbial fuel cells (MFCs). Complete genome sequencing of P. stutzeri and cyclic voltammetry method were performed to reveal its mechanism in microbial fuel cells system. Results This study indicated that the MFCs generated a maximum output voltage of 254.2 mV and 226.0 mV, and maximum power density of 765 mW/m2 and 656.6 mW/m2 respectively. Complete genome sequencing of P. stutzeri S116 was performed to indicate that most function genes showed high similarities with P. stutzeri, and its primary annotations were associated with energy production and conversion (6.84%), amino acid transport and metabolism (6.82%) and inorganic ion transport and metabolism (6.77%). Homology of 36 genes involved in oxidative phosphorylation was detected, which suggests the strain S116 possesses an integrated electron transport chain. Additionally, many genes encoding pilus-assembly proteins and redox mediators (riboflavin and phenazine) were detected in the databases. Thiosulfate oxidization and dissimilatory nitrate reduction were annotated in the sulfur metabolism pathway and nitrogen metabolism pathway, respectively. Gene function analysis and cyclic voltammetry indicated that P. stutzeri probably possesses cellular machinery such as cytochrome c and redox mediators and can perform extracellular electron transfer and produce electricity in MFCs. Conclusion The redox mediators secreted by P. stutzeri S116 were probably responsible for performance of MFCs. The critical genes and metabolic pathways involved in thiosulfate oxide and nitrate reduction were detected, which indicated that the strain can treat wastewater containing sulfide and nitrite efficiently.

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Section: Discussionmentioning

confidence: 99%

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

et al. 2022

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“…Overall, compost induced the increase in abundance of some proteins involved in amino acid metabolism, suggesting a key role of these amino acids in synthesis of various proteins that are required for barley plants growth and development adjustement at the tillering stage. In addition to the classic role of amino acids in the synthesis of proteins, biosynthesis of amino acids such as lysine and isoleucine as well as sulfur/nitrogen containing amino acids like methionine supports plant energy requirements for growth and development [ 59 ]. Under energy deficiency, lysine and isoleucine catabolism flux directly into the tricarboxylic acid (TCA) cycle, while methionine is converted into isoleucine [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Barley (Hordeum vulgare L.) Leaves in Response to Date Palm Waste Compost Application

Ghouili

Abid

Jebara

et al. 2022

Plants

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Composts are an emerging biofertilizers used in agronomy that can improve crop performance, but much less is known regarding their modes of action. The current study aimed to investigate the differentially abundant proteins (DAPs) in barley leaves associated with growth promotion induced by application of date palm waste compost. Morphophysiological measurements revealed that compost induced a significant increase in plant height, chlorophyll content, gas exchange parameters and plant biomass. LC-MS/MS analyses indicate that compost induced global changes in the proteome of barley leaves. A total of 62 DAPs (26 upregulated and 36 downregulated) among a total of 2233 proteins were identified in response to compost application. The expression of DAPs was further validated based on qRT-PCR. Compost application showed altered abundance of several proteins related to abiotic stress, plant defense, redox homeostasis, transport, tricarboxylic acid cycle, carbohydrate, amino acid, energy and protein metabolism. Furthermore, proteins related to metabolic processes of phytohormone, DNA methylation and secondary metabolites were induced. These results indicate that barley responds to compost application by complex metabolism pathways and may result in a positive alteration in a physiological and metabolic barley plant state which consequently could lead to improved growth and stress adaptation observed in compost-treated plants.

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“…The nitrogen metabolism pathway indicates that functional denitri cation genes such as nirS (playing an important role in nitrite reduction), norB, nosZ, and narG (Chen et al 2020) are detected in P. stutzeri S116 with denitri cation. In addition, nitrate is metabolized into ammonia through dissimilatory nitrate reduction, and the annotated gene nirBD encodes nitrite reductase (EC:1.7.1.15) for nitrite reduction(Shi et al 2019).…”

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confidence: 99%

Complete Genome Sequence of Pseudomonas Stutzeri S116 Provides Insights into the Mechanism of Microbial Fuel Cells

Yuan

Huang

et al. 2022

Preprint

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To identify suitable biocatalysts applied in microbial fuel cells (MFCs), Pseudomonas stutzeri S116 isolated from marine sludge was investigated, which possessed excellcent bioelectricity generation ability (BGA). Herein, P. stutzeri as a bioanode and biocathode achieved maximum output voltage (254.2 mV and 226.0 mV), and power density of (765 mW/m2 and 656.6 mW/m2). Complete genome sequencing of P. stutzeri was performed to reveal its potential microbial functions. The results exhibited that the strain was the ecologically dominant Pseudomonas, and its primary annotations were associated with energy production and conversion (6.84%), amino acid transport and metabolism (6.82%) and inorganic ion transport and metabolism (6.77%). The thirty-six genes involved in oxidative phosphorylation indicate that strain possesses an integrated electron transport chain. Moreover, many genes encoding redox mediators (mainly riboflavin and phenazine) were detected in the databases. Simultaneously, thiosulfate oxidization and dissimilatory nitrate reduction were annotated in the sulfur metabolism and nitrogen metabolism pathway. Gene function and cyclic voltammetry (CV) analysis indicated BGA of P. stutzeri probably was attributed to its cytochrome c and redox mediators, which enhance extracellular electron transfer (EET) rate.

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Cloning of nitrite reductase gene from Haematococcus pluvialis and transcription and enzymatic activity analysis at different nitrate and phosphorus concentration

Cited by 6 publications

References 29 publications

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

Proteomic Analysis of Barley (Hordeum vulgare L.) Leaves in Response to Date Palm Waste Compost Application

Complete Genome Sequence of Pseudomonas Stutzeri S116 Provides Insights into the Mechanism of Microbial Fuel Cells

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