Cloning of notch1 and its role in the growth and development of Exopalaemon carinicauda

Shen, Shanrui; Hu, Jian; Shen, Qin; Chen, Hao; Gao, Huan; Lai, Xiaofang

doi:10.1016/j.aqrep.2023.101537

Cited by 5 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method has also been explored by our predecessors. Studies have used RNAi technology to interfere with the notch1 gene and have calculated the gonadal index after ten days of cultivation to determine that the notch1 gene affects the development of the ovaries in Exopalaemon carinicauda [45].…”

Section: Discussionmentioning

confidence: 99%

Cloning, Identification, and Functional Analysis of the Foxl2 Gene in Procambarus clarkii

Huang,

Zhu,

Peng

et al. 2023

Genes

View full text Add to dashboard Cite

Procambarus clarkii is the most widely distributed freshwater shrimp in China, with important economic value and great potential for development. The forkheadboxL2 (Foxl2) gene has been found to be involved in the reproductive development of many crustaceans. To understand the role of the Foxl2 gene in the gonad development of P. clarkii, we designed CDS-specific primers for the P. clarkii Foxl2 (PcFoxl2) gene and cloned its CDS sequence using RT-PCR. The nucleotide and protein sequence information was then analyzed through bioinformatics analysis. The expression and subcellular localization of PcFoxl2 in various tissues were detected using qRT-PCR and in situ hybridization. The effects of PcFoxl2 knockdown on gonad development were investigated using RNA interference. The results showed that the CDS length of the PcFoxl2 gene was 1614 bp and encoded 537 amino acids. Protein sequence comparison and phylogenetic analysis showed that PcFoxl2 was the closest relative to Crayfish. qRT-PCR analysis indicated that the expression level of PcFoxl2 in the testis was significantly higher (>40 fold) than that in the ovary (p < 0.01). The in situ hybridization results showed that PcFoxl2 was expressed in both the cytoplasm and the nucleus of egg cells, and that the expression was strongest in egg cells at the early stage of yolk synthesis, while weak in the secondary oocytes. The positive signal was strongest in the spermatocyte nucleolus, while only a trace signal was observed in the cytoplasm. After interfering with the PcFoxl2 gene using dsRNA, the expression of PcFoxl2 in the RNA interference group was significantly lower than that in the control group, and this interference effect lasted for one week. Moreover, the gonad index of the experimental group was significantly lower than that of the control group (p < 0.05) after 10 days of P. clarkii cultivation following PcFoxl2 knockdown. The expression levels of the nanos and S3a genes, which are related to gonad development, decreased significantly after PcFoxl2 gene interference. The results suggest that the Foxl2 gene is involved in the growth and development of gonads, particularly in the development of testis, and is related to the early development of oocytes. This study provides a theoretical basis for the artificial breeding of P. clarkii.

show abstract

Section: Discussionmentioning

confidence: 99%

Cloning, Identification, and Functional Analysis of the Foxl2 Gene in Procambarus clarkii

Huang,

Zhu,

Peng

et al. 2023

Genes

View full text Add to dashboard Cite

show abstract

“…The average body length and body weight of the E. carinicauda individuals were 4.5 ± 0.5 cm and 1.4 ± 0.4 g, respectively. All the samples were acclimated for two weeks with stable salinity (27)(28)(29) and pH (8.1-8.4). The shrimp were fed twice daily with commercial pellets and the filtered seawater was renewed every two days.…”

Section: Samplesmentioning

confidence: 99%

“…Exopalaemon carinicauda , also known as the ridgetail white prawn, is distributed mainly on the coast of mainland China and the west coast of the Korean Peninsula [ 23 , 24 ]. E. carinicauda has the advantages of a strong reproductive ability, a short growth cycle, and a long breeding season, so it represents an important economic breeding variety in China, the annual production of which exceeds 50,000 tons, and the output value exceeds USD 420 million [ 25 , 26 , 27 , 28 ]. Like most other crustaceans, E. carinicauda has the powerful ability to regenerate severed limbs.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda

Xing,

Wang,

Chen

et al. 2024

Animals

Self Cite

View full text Add to dashboard Cite

With the increase in breeding density of Exopalaemon carinicauda, appendage breakage may occur, which seriously affects survival and economic benefits. To study the limb regeneration process of E. carinicauda, we induced autotomy of the pereopods. After a period of time, wound swelling disappeared, the pigment gradually accumulated, and a tawny film subsequently formed in the wound. The healing period of the wound occurred 24 h after autotomy, and the blastema formation stage occurred 48 h after autotomy. After 4 days of cutting, the limb buds began to differentiate, grow, and expand rapidly, and this process lasted approximately 15 days. Microscopic observations revealed significant changes in the type and number of associated cells including outer epithelial cells, granulocytes, embryonic cells, columnar epidermal cells, elongated cells, and blastoma cells, during the process from limb fracture to regeneration. A comparative transcriptome analysis identified 1415 genes differentially expressed between the J0h (0 h post autotomy) and J18h (18 h post autotomy), and 3952 and 4366 differentially expressed genes for J0 and J14d (14 days post autotomy) and J18h and J14d, respectively. Some of these genes may be related to muscle growth or molting, as indicated by the presence of troponin C, chitinase, actin, innexin, and cathepsin L. As a functional gene involved in epidermal formation, the mRNA expression level of the innexin inx2 in the pereopod of E. carinicauda changed significantly in the experimental groups (p < 0.05). The results of this study contribute to existing knowledge of regeneration mechanisms in crustaceans.

show abstract

Denitrification characteristics and potential application of a novel aerobic denitrifying bacterium, Pseudomonas plecoglossicida, isolated from the Exopalaemon carinicauda pond

Shen,

Zhou,

Qian

et al. 2024

Aquacult Int

View full text Add to dashboard Cite

Cloning of notch1 and its role in the growth and development of Exopalaemon carinicauda

Cited by 5 publications

References 54 publications

Cloning, Identification, and Functional Analysis of the Foxl2 Gene in Procambarus clarkii

Cloning, Identification, and Functional Analysis of the Foxl2 Gene in Procambarus clarkii

Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda

Denitrification characteristics and potential application of a novel aerobic denitrifying bacterium, Pseudomonas plecoglossicida, isolated from the Exopalaemon carinicauda pond

Contact Info

Product

Resources

About