Cloning of Precursors for Two MIH/VIH-Related Peptides in the Prawn, Macrobrachium rosenbergii

Yang, Wei‐Jun; Rao, K. Ranga

doi:10.1006/bbrc.2001.6019

Cited by 34 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, VIH has only been found in a few crustacean species (Fig.3) such as Homarus gammarus (Ollivaux et al, 2006), H. americanus , the Norway lobster Nephrops norvegicus (Edomi et al, 2002), the giant river prawn Macrobrachium rosenbergii (Yang and Rao, 2001), the shrimp Rimicaris kairei (Qian et al, 2009) and the woodlouse Armadillidium vulgare (Greve et al, 1999). Molecular analysis of VIHs isolated from females of a few crustacean species shows that they consist of signal peptides (20-31 amino acid residues) and mature peptides (77-83 amino acid residues; Fig.3).…”

Section: The Journal Of Experimental Biologymentioning

confidence: 99%

Reproductive regulators in decapod crustaceans: an overview

Nagaraju

2011

Journal of Experimental Biology

271

170

View full text Add to dashboard Cite

SummaryControl of reproductive development in crustaceans requires neuropeptides, ecdysone and methyl farnesoate (MF). A major source of neuropeptides is the X-organ-sinus gland (XO-SG) complex located in the eyestalk ganglia of crustaceans. The other regulatory factors (either peptides or neuromodulators) are produced in the brain and thoracic ganglia (TG). Two other regulatory non-peptide compounds, the steroid ecdysone and the sesquiterpene MF, are produced by the Y-organs and the mandibular organs, respectively. In the current review, I have tried to recapitulate recent studies on the role of gonadal regulatory factors in regulating crustacean reproduction.Key words: crustaceans, reproduction, gonads, X-organ-sinus gland, peptides. THE JOURNAL OF EXPERIMENTAL BIOLOGY4 crustaceans, the testis contains 10-15 lobes each composed of many seminiferous tubules, whose shape changes according to the stage of spermatogenesis. Spermatogenesis starts in the undifferentiated seminiferous tubules (proacini) at the top of each lobe. Meiotic activity occurs in the acini that develop proacini somewhat proximally from the top of each lobe. In this stage, the lobules contain mostly primary spermatocytes. Differentiation of secondary spermatocytes and spermatids takes place in the seminiferous tubules, which lie in the center of each lobe. All these seminiferous tubules are filled with spermatocytes and spermatids surrounded by a layer of otheocytes of mesodermal origin. The hormonal control of spermatogenesis is not completely understood in crustaceans. There is information on MF as a reproductive hormone in males. The female reproductive systemThe reproductive system of female crustaceans consists of the paired ovaries, oviducts, gonophores and an external sperm reception area. The ovarian lobes are connected by a central bridge of ovarian tissue (Fig.1D). The ovarian lobes are symmetrically arranged and lie in the cephalothorax on the top of the stomach and hepatopancreas (HP). An oviduct arises laterally from each ovary at a point just beside the position of the heart. It extends ventrally and opens through a gonophore in the abdomen. In sexually receptive females, each pore is equipped with a large tuft of long setae, which apparently serve as a tube for the passage of ova. In the fully mature state, the shapes of ovarian limbs can be distinguished only with difficulty as the ovary fills the thoracic region of the body cavity completely.In the majority of female Malacostraca, oogonial proliferation and ovarian differentiation take place when the ovary is translucent to opaque white (previtellogenic ovary). During vitellogenesis the color of the ovary changes from pale yellow (vitellogenic stage I) to orange (vitellogenic stage II) and it then becomes brown (vitellogenic stage III) to dark brown prior to spawning. Maturation of the ovary also includes an increase in the size of the ovary as the oocytes proliferate and increase in diameter, due to yolk deposition. Role of neuropeptides in reproductionThe hormonal control o...

show abstract

Section: The Journal Of Experimental Biologymentioning

confidence: 99%

Reproductive regulators in decapod crustaceans: an overview

Nagaraju

2011

Journal of Experimental Biology

271

170

View full text Add to dashboard Cite

show abstract

“…The preprohormones of the CHH subgroup always contain a second peptide, commonly referred to as CHH precursor-related peptide or CPRP, which is Cterminally flanked by the CHH (Weidemann et al, 1989;Tensen et al, 1991;de Kleijn et al, 1994a;Ohira et al, 1997a;Davey et al, 2000;Toullec et al, 2002;Marco et al, 2003;Chen et al, 2004;Mettulio et al, 2004;de la Iglesia et al, 2005;Hsu et al, 2005a;Toullec et al, 2006). The preprohormones of the MIH/GIH/VIH/MOIH sub-group lack precursor-related peptides (Klein et al, 1993;de Kleijn et al, 1994b;Sun, 1994;Lee et al, 1995;Ohira et al, 1997b;Chan et al, 1998;Gu and Chan, 1998;Umphrey et al, 1998;Tang et al, 1999;Lu et al, 2001;Yang and Rao, 2001;Edomi et al, 2002;Krungkasem et al, 2002;Ohira et al, 2005). Although CPRP has been shown to circulate in the hemolymph, the physiological role(s) it plays in crustaceans remains unknown (Wilcockson et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs ofCancercrabs: implications for differential release and pleiotropic function

Hsu

Messinger

Chung

et al. 2006

Journal of Experimental Biology

View full text Add to dashboard Cite

Research on crustaceans has played a major role in advancing our understanding of neuroendocrine structure and control. Nearly 80 years ago, Koller (Koller, 1925;Koller, 1927) demonstrated that hemolymph-borne agents were responsible for color change in shrimp. In the decade that followed, Hanström (Hanström, 1931;Hanström, 1933;Hanström, 1935;Hanström, 1937) identified a structure located in the eyestalk, the sinus gland (SG) as the source of these substances. Later, Bliss (Bliss, 1951) and Passano (Passano, 1951) independently demonstrated the neurohemal nature of the SG, a first for any neural structure in the animal kingdom. In recent years, work from many laboratories has shown that the SG is one of the major neuroendocrine organs in crustaceans and a number of hormones contained within it have been identified, including a group of structurally related peptides that have collectively been termed the crustacean hyperglycemic hormone (CHH) family. The crustacean hyperglycemic hormone (CHH) family of peptides includes CHH, moult-inhibiting hormone (MIH) and mandibular organ-inhibiting hormone (MOIH). In the crabCancer pagurus, isoforms of these peptides, as well as CHH precursor-related peptide (CPRP), have been identified in the X-organ-sinus gland (XO-SG) system. Using peptides isolated from the C. pagurus SG, antibodies to each family member and CPRP were generated. These sera were then used to map the distributions and co-localization patterns of these peptides in the neuroendocrine organs of seven Cancer species: Cancer antennarius, Cancer anthonyi, Cancer borealis, Cancer gracilis, Cancer irroratus, Cancer magister and Cancer productus. In addition to the XO-SG, the pericardial organ (PO) and two other neuroendocrine sites contained within the stomatogastric nervous system, the anterior cardiac plexus (ACP) and the anterior commissural organ (ACO), were studied. In all species, the peptides were found to be differentially distributed between the neuroendocrine sites in conserved patterns: i.e. CHH, CPRP, MIH and MOIH in the XO-SG, CHH, CPRP and MOIH in the PO, and MOIH in the ACP (no immunolabeling was found in the ACO). Moreover, in C. productus (and probably in all species), the peptides present in the XO-SG and PO were differentially distributed between the neurons within each of these neuroendocrine organs (e.g. CHH and CPRP in one set of XO somata with MIH and MOIH co-localized in a different set of cell bodies). Taken collectively, the differential distributions of CHH family members and CPRP both between and within the neuroendocrine organs of crabs of the genus Cancer suggests that each of these peptides may be released into the circulatory system in response to varied, tissue-specific cues and that the POand/or ACP-derived isoforms may possess functions distinct from those classically ascribed to their release from the SG.

show abstract

“…The cDNA and amino acid sequences of GIH/VIH and GIH-like peptides have been reported in several crustacean species (Edomi et al 2002;Treerattrakool et al 2008;Yang and Rao 2001;Chen et al 2014). In the present study, the we showed that the primary structure of the mature LvGIH peptide consisted of 79 amino acids containing three disulfide bonds at C7-C44, C24-C40 and C27-C53, which is typical for type II CHH family peptides (Webster et al 2012).…”

Section: Discussionmentioning

confidence: 99%

cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei

Deng

Jiang

et al. 2015

Genes Genom

View full text Add to dashboard Cite

In this study, the full-length GIH cDNA sequence from Litopenaeus vannamei was cloned from the eyestalk by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The fulllength GIH cDNA was 865 bp with a 288 bp open-reading frame, which encoded a 96 amino acid prepro-GIH with 17 amino acid signal peptide. L. vannamei GIH (LvGIH) can be classified as a member of type-II crustacean hyperglycemic hormone polypeptide family. LvGIH shares 93.8 and 66.7 % amino acid sequence identity with GIH from Penaeus monodon, and the molt-inhibiting hormone from Marsupenaeus japonicas, respectively. By quantitative real-time PCR (qPCR), LvGIH mRNA transcripts were detected in fertilized eggs, nauplius, zoea, mysis and juveniles of 25, 35 and 40 days old. LvGIH transcript levels increased significantly with the development from fertilized eggs to juveniles. LvGIH transcript levels were highest in juveniles at 35 days old. By RT-PCR, LvGIH mRNA transcripts were detected only in the eyestalks and brains but not in the muscles, intestines, gills, heart, hepatopancreas, ovaries and testes of adults, and there was no difference in the expression level of LvGIH between males and females. Using the P. monodon anti-GIH antibody, we showed that LvGIH was located mainly in the XO-SG and slightly in axon, with similar fluorescence intensity found in XO and SG. To summarize, we have cloned and characterized the GIH of the shrimp L. vannamei. In addition to the GIH properties described in other crustaceans, a peak of LvGIH expression was identified at the time of sexual differentiation (i.e., day 35 larvae) suggesting that LvGIH may also be involved in the control of this process.

show abstract

Cloning of Precursors for Two MIH/VIH-Related Peptides in the Prawn, Macrobrachium rosenbergii

Cited by 34 publications

References 38 publications

Reproductive regulators in decapod crustaceans: an overview

Reproductive regulators in decapod crustaceans: an overview

Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs ofCancercrabs: implications for differential release and pleiotropic function

cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei

Contact Info

Product

Resources

About