Cloning of stanniocalcin (STC) cDNAs of divergent teleost species: Monomeric STC supports monophyly of the ancient teleosts, the osteoglossomorphs

Amemiya, Yutaka; Irwin, David M.; Youson, John H.

doi:10.1016/j.ygcen.2006.03.015

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…The apparent size of the immunoreactive band under denaturing reducing conditions is 3 kDa smaller than the purified STC (26 kDa). This apparent disparity in size is probably related to the removal of the 30 amino acid signal peptide in the N-terminus of STC, as previously described for other fish species (Amemiya et al, 2006;Amemiya et al, 2002). Preabsorption of the sea bream anti-STC sera with a protein extract of isolated Stannius corpuscles ablated the immunoreactive band obtained in western blots of serum and purified STC, and confirms antisera specificity.…”

Section: Stc Antiserum Validationsupporting

confidence: 78%

Endocrine regulation of carbonate precipitate formation in marine fish intestine by Stanniocalcin and PTHrP

Gregório

Carvalho

Campinho

et al. 2014

Journal of Experimental Biology

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In marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. In vitro studies highlight the involvement of the calciotropic hormones PTHrP (parathyroid hormone-related protein) and stanniocalcin (STC) in the regulation of epithelial bicarbonate transport. The present study tested the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo. Sea bream (Sparus aurata) juveniles received single intraperitoneal injections of piscine PTHrP(1-34), the PTH/PTHrP receptor antagonist PTHrP(7-34) or purified sea bream STC, or were passively immunized with polyclonal rabbit antisera raised against sea bream STC (STC-Ab). Endocrine effects on the expression of the basolateral sodium bicarbonate cotransporter (Slc4a4.A), the apical anion exchangers Slc26a6.A and Slc26a3.B, and the V-type proton pump β-subunit (Atp6v1b) in the anterior intestine were evaluated. In keeping with their calciotropic nature, the hypocalcaemic factors PTHrP(7-34) and STC upregulated gene expression of all transporters. In contrast, the hypercalcaemic factor PTHrP(1-34) and STC antibodies downregulated transporters involved in the bicarbonate secretion cascade. Changes in intestine luminal precipitate contents provoked by calcaemic endocrine factors validated these results: 24 h postinjection either PTHrP(1-34) or immunization with STC-Ab reduced the carbonate precipitate content in the sea bream intestine. In contrast, the PTH/PTHrP receptor antagonist PTHrP(7-34) increased not only the precipitated fraction but also the concentration of HCO 3 -equivalents in the intestinal fluid. These results confirm the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo in the intestine of marine fish. Furthermore, they illustrate for the first time in fish the counteracting effect of PTHrP and STC, and reveal an unexpected contribution of calcaemic factors to acid-base balance.

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Section: Stc Antiserum Validationsupporting

confidence: 78%

Endocrine regulation of carbonate precipitate formation in marine fish intestine by Stanniocalcin and PTHrP

Gregório

Carvalho

Campinho

et al. 2014

Journal of Experimental Biology

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“…General information about calciotropic hormones, osmoregulation, and nutrition in teleosts can be found in Urist et al (1972), Flik & Verbost (1995), Jurss & Bastrop (1995), Sasayama (1999), Perry et al (2003) and Lall & Lewis‐McCrea (2007). Other studies focus on particular hormones and vitamins that can influence teleost bone metabolism: calcitonin (Lopez et al , 1976; Arlot‐Bonnemains & Fouchereau‐Peron, 1984), stanniocalcin (Verbost & Fenwick, 1995; Barlet et al , 1998; Amemiya, Irwin & Youson, 2006), vitamin D (Graff et al , 2002), thyroid hormone (Sæle et al , 2003; Okada, Tanaka & Tagawa, 2003; Takagi et al , 1994; Sbaihi et al , 2007), parathyroid hormone and parathyroid hormone‐related protein (Danks et al , 1993, 2003; Trivett et al , 1999; Guerreiro et al , 2007), prolactin (Power, 2005), estrogen and testosterone (Urasa et al , 1984; Persson et al , 2000; Guerreiro et al , 2002), melatonin (Suzuki & Hattori, 2002; Fjelldal et al , 2004), leptin (Baker et al , 2000), and growth hormone (Takagi et al , 1992). It has been emphasised for calcitonin, estradiol‐17β (E 2 ) and vitamin D, that the function of calcium‐regulating factors in teleosts is not fully understood and conflicting results have been published (Wagner, Jaworski & Radman, 1997; Persson et al , 1997; Lall & Lewis‐McCrea, 2007).…”

Section: The Functions Of Teleost Skeletal Remodellingmentioning

confidence: 99%

A comparative view on mechanisms and functions of skeletal remodelling in teleost fish, with special emphasis on osteoclasts and their function

2009

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Resorption and remodelling of skeletal tissues is required for development and growth, mechanical adaptation, repair, and mineral homeostasis of the vertebrate skeleton. Here we review for the first time the current knowledge about resorption and remodelling of the skeleton in teleost fish, the largest and most diverse group of extant vertebrates. Teleost species are increasingly used in aquaculture and as models in biomedical skeletal research. Thus, detailed knowledge is required to establish the differences and similarities between mammalian and teleost skeletal remodelling, and between distantly related species such as zebrafish (Danio rerio) and medaka (Oryzias latipes). The cellular mechanisms of differentiation and activation of osteoclasts and the functions of teleost skeletal remodelling are described. Several characteristics, related to skeletal remodelling, distinguish teleosts from mammals. These characteristics include (a) the absence of osteocytes in most species; (b) the absence of haematopoietic bone marrow tissue; (c) the abundance of small mononucleated osteoclasts performing non-lacunar (smooth) bone resorption, in addition to or instead of multinucleated osteoclasts; and (d) a phosphorus- rather than calcium-driven mineral homeostasis (mainly affecting the postcranial dermal skeleton). Furthermore, (e) skeletal resorption is often absent from particular sites, due to sparse or lacking endochondral ossification. Based on the mode of skeletal remodelling in early ontogeny of all teleosts and in later stages of development of teleosts with acellular bone we suggest a link between acellular bone and the predominance of mononucleated osteoclasts, on the one hand, and cellular bone and multinucleated osteoclasts on the other. The evolutionary origin of skeletal remodelling is discussed and whether mononucleated osteoclasts represent an ancestral type of resorbing cells. Revealing the differentiation and activation of teleost skeletal resorbing cells, in the absence of several factors that trigger mammalian osteoclast differentiation, is a current challenge. Understanding which characters of teleost bone remodelling are derived and which characters are conserved should enhance our understanding of the process in fish and may provide insights into alternative pathways of bone remodelling in mammals.

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“…In ancient fish, the last conserved cysteine residue in the C-terminal of STC1, which is supposedly involved in its dimerization, is replaced by arginine or histidine residues, thereby giving rise to a strictly monomeric form of the protein [ 42 , 43 ]. Although dimeric forms of STC1 have been described [ 39 , 44 , 45 ], answers to the question of its potential multimerization and modification to diverse higher molecular weight forms under certain circumstances remain elusive.…”

Section: Introductionmentioning

confidence: 99%

Low-resolution structural studies of human Stanniocalcin-1

Trindade

Silva

Navarro³

et al. 2009

BMC Struct Biol

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Background: Stanniocalcins (STCs) represent small glycoprotein hormones, found in all vertebrates, which have been functionally implicated in Calcium homeostasis. However, recent data from mammalian systems indicated that they may be also involved in embryogenesis, tumorigenesis and in the context of the latter especially in angiogenesis. Human STC1 is a 247 amino acids protein with a predicted molecular mass of 27 kDa, but preliminary data suggested its di-or multimerization. The latter in conjunction with alternative splicing and/or post-translational modification gives rise to forms described as STC 50 and "big STC", which molecular weights range from 56 to 135 kDa.

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Cloning of stanniocalcin (STC) cDNAs of divergent teleost species: Monomeric STC supports monophyly of the ancient teleosts, the osteoglossomorphs

Cited by 9 publications

References 16 publications

Endocrine regulation of carbonate precipitate formation in marine fish intestine by Stanniocalcin and PTHrP

Endocrine regulation of carbonate precipitate formation in marine fish intestine by Stanniocalcin and PTHrP

A comparative view on mechanisms and functions of skeletal remodelling in teleost fish, with special emphasis on osteoclasts and their function

Low-resolution structural studies of human Stanniocalcin-1

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