Cloning of the nitrate - nitrite reductase gene cluster of Penicillium chrysogenum and use of the niaD gene as a homologous selection marker

Gouka, Robin J.; Hartingsveldt, W. van; Bovenberg, Roel A. L.; Hondel, Cees A. M. J. J. van den; Gorcom, R.F.M. van

doi:10.1016/0168-1656(91)90227-m

Cited by 30 publications

(18 citation statements)

References 25 publications

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“…P. chrysogenum strains used in this study (listed in Table 1) were cultured as described by Gouka et al (1991). P. chrysogenum transformants were selected on media with acetamide as sole nitrogen source as described by Cantoral et al (1987).…”

Section: Strainsmentioning

confidence: 99%

Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorpha

Kiel¹,

Berg

Bovenberg³

et al. 2004

Fungal Genetics and Biology

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Section: Strainsmentioning

confidence: 99%

Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorpha

Kiel¹,

Berg

Bovenberg³

et al. 2004

Fungal Genetics and Biology

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“…Single colonies were selected, sporulated, streaked out to single spores, and subsequently analyzed for the expression of the transformed constructs by fluorescence microscopy and/or Western blotting. Nitrate reductase (niaD)-deficient strains were isolated by using chlorate plates using adenine as a nitrogen source (6).…”

Section: Methodsmentioning

confidence: 99%

Peroxisomes Are Required for Efficient Penicillin Biosynthesis in Penicillium chrysogenum

Meijer

Gidijala

Fekken

et al. 2010

Appl Environ Microbiol

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In the fungus Penicillium chrysogenum, penicillin (PEN) production is compartmentalized in the cytosol and in peroxisomes. Here we show that intact peroxisomes that contain the two final enzymes of PEN biosynthesis, acyl coenzyme A (CoA):6-amino penicillanic acid acyltransferase (AT) as well as the side-chain precursor activation enzyme phenylacetyl CoA ligase (PCL), are crucial for efficient PEN synthesis. Moreover, increasing PEN titers are associated with increasing peroxisome numbers. However, not all conditions that result in enhanced peroxisome numbers simultaneously stimulate PEN production. We find that conditions that lead to peroxisome proliferation but simultaneously interfere with the normal physiology of the cell may be detrimental to antibiotic production. We furthermore show that peroxisomes develop in germinating conidiospores from reticule-like structures. During subsequent hyphal growth, peroxisome proliferation occurs at the tip of the growing hyphae, after which the organelles are distributed over newly formed subapical cells. We observed that the organelle proliferation machinery requires the dynamin-like protein Dnm1.

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“…As for Aspergillus nidulans and Aspergillus fumigatus nitrate assimilation genes (crnA encoding nitrate transporter, niaD encoding nitrate reductase, niiA encoding nitrite reductase) are described to be clustered, 20,21) whereas in the case of Penicillium chrysogenum, only the nitrate-nitrite reductase gene cluster has been isolated. 22) In PP-V production using nitrate, nitrate assimilation is an important process, but no nitrate assimilation-related gene cluster has been identified in Penicillium spp.…”

Section: In An Ammonium Nitrate-free Medium Pp-o [(10z)-12-carboxyl-mentioning

confidence: 99%

Effects of Inorganic Nitrogen Sources on the Production of PP-V [(10Z)-12-carboxyl-monascorubramine] and the Expression of the Nitrate Assimilation Gene Cluster byPenicilliumsp. AZ

Arai

Umemura

Ota

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Cloning of the nitrate - nitrite reductase gene cluster of Penicillium chrysogenum and use of the niaD gene as a homologous selection marker

Cited by 30 publications

References 25 publications

Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorpha

Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorpha

Peroxisomes Are Required for Efficient Penicillin Biosynthesis in Penicillium chrysogenum

Effects of Inorganic Nitrogen Sources on the Production of PP-V [(10Z)-12-carboxyl-monascorubramine] and the Expression of the Nitrate Assimilation Gene Cluster byPenicilliumsp. AZ

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