Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer

Zhang, Ruifu; Cui, Zhongli; Zhang, Xiaozhou; Jiang, Jiandong; Gu, Ji‐Dong; Li, Shunpeng

doi:10.1007/s10532-005-9018-6

Cited by 72 publications

(40 citation statements)

References 28 publications

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“…Such variations attributed to the increased hydrolytic efficiency and hydrolysis rate for chlorpyrifos compared to other tested substrates such as profenofos. Zhang et al (2006) have noticed mpd gene cluster that constituted catabolic transposon, which contribute to the dispersion and expression of this gene among a variety of indigenous bacteria in a polluted environment.…”

Section: Biodegradation At Enzyme and Molecular Levelsmentioning

confidence: 99%

Chlorpyrifos: pollution and remediation

2015

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The widespread use of pesticides in modern agriculture is of increasing concern due to environmental contamination and subsequent biodiversity loss. Chlorpyrifos is a toxic organophosphate pesticide. Repeated applications of chlorpyrifos modify the soil microbial community structure and pose potential health risks to the other nontargets. Chlorpyrifos has been reported as the second most commonly detected pesticide in food and water. Extensive use of chlorpyrifos in agriculture and persistence in the environment have raised public concern and demand for safe technologies to overcome the pollution and toxicity problems. Here, we review pollution and toxicity issues associated with chlorpyrifos use and discuss strategies to solve pesticide contamination. Chlorpyrifos, previously shown to be resistant to enhanced degradation, has now been proved to undergo enhanced microbe-mediated decay. Here, special emphasis is given to degradation methods such as ozonation, Fenton treatment, photodegradation, and advanced oxidation processes along with microbial degradation. Finally, we focus on degradation process at enzyme and molecular levels which will enable us to elucidate the exact degradative pathway involved in biodegradation.

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Section: Biodegradation At Enzyme and Molecular Levelsmentioning

confidence: 99%

Chlorpyrifos: pollution and remediation

2015

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“…Methyl parathion-degrading gene (mpd) has been intensively researched (Cui et al 2001;Fu et al 2004;Rani and Lalithakumari 1994;Yang et al 2006;Zhang et al 2006). We previously cloned mpd gene from chlorpyrifos-degrading Stenotrophomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates

Yang

Jiang

et al. 2008

Biodegradation

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The coding region of mpd gene corresponding to mature methyl parathion hydrolase (MPH) was heterologously overexpressed in Escherichia coli BL21 (DE3) by using pET expression system. The lactose-induced expression yield of MPH is increased 2-fold compared with IPTG as inducer. Furthermore, it was found that specific activity of MPH increased 48% by reducing the induction temperature to 22 degrees C. The addition of 25 mM lactose at 22 degrees C, the MPH activity of fermentation broth had a specific activity of 1.4 x 10(4) U/mg protein. Plasmid was no significant decrease in the modified medium. The optimal pH and temperature of MPH were 8.0 and 30 degrees C, respectively. Over a period of 5 months, the dried cells showed no significant decrease in the activity of the detoxifying enzymes. The crude enzymes in 50 mM citrate-phosphate buffer (pH 8.0) were able to degrade about 98% of the organophosphate pesticides sprayed on cabbage. The detoxification efficiency was superior to that of the treatments of water, detergent, and a commercially available enzyme product. Additionally, the products of pesticide hydrolysis generated by treatment with the enzyme extract were determined to be virtually nontoxic.

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“…Moreover, MPH is believed to be a recent product of molecular evolution; it has probably appeared after the use of pesticides began about 50 years ago. Thus far, it has been found only in a limited number of bacterial strains [25][26][27]; its occurrence in animals and plants has not yet been reported. Besides, MPH is not a constructive enzyme but an inductive enzyme; this implies that bacterial cells would not produce MPH unless methyl parathion is introduced in its environment for a certain time period.…”

Section: Discussionmentioning

confidence: 99%

Application of methyl parathion hydrolase (MPH) as a labeling enzyme

et al. 2008

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Methyl parathion hydrolase (MPH) is an enzyme that catalyzes the degradation of methyl parathion, generating a yellow product with specific absorption at 405 nm. The application of MPH as a new labeling enzyme was illustrated in this study. The key advantages of using MPH as a labeling enzyme are as follows: (1) unlike alkaline phosphatase (AP), horseradish peroxidase (HRP), and glucose oxidase (GOD), MPH is rarely found in animal cells, and it therefore produces less background noise; (2) its active form in solution is the monomer, with a molecular weight of 37 kDa; (3) its turnover number is 114.70±13.19 s −1 , which is sufficiently high to yield a significant signal for sensitive detection; and (4) its 3D structure is known and its C-terminal that is exposed to the surface can be easily subjected to the construction of genetic engineering monocloning antibody-enzyme fusion for enzyme-linked immunosorbent assay (ELISA). To demonstrate its utility, MPH was ligated to an single-chain variable fragment (scFv), known as A1E, against a white spot syndrome virus (WSSV) with the insertion of a [-(Gly-Ser) 5 -] linker peptide. The resulting fusion protein MPH-A1E possessed both the binding specificity of the scFv segment and the catalytic activity of the MPH segment. When MPH-A1E was used as an ELISA reagent, 25 ng purified WSSV was detected; this was similar to the detection sensitivity obtained using A1E scFv and the HRP/Anti-E Tag Conjugate protocol. The fusion protein also recognized the WSSV in 1 μL hemolymph from an infected shrimp and differentiated it from a healthy shrimp.

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Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer

Cited by 72 publications

References 28 publications

Chlorpyrifos: pollution and remediation

Chlorpyrifos: pollution and remediation

Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates

Application of methyl parathion hydrolase (MPH) as a labeling enzyme

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