1998
DOI: 10.1016/s0378-1097(98)00119-0
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Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli

Abstract: Phytase hydrolyzes phytate to release inorganic phosphate, which would decrease the addition of phosphorus to feedstuffs for monogastric animals and thus reduce environmental pollution. The gene encoding phytase from Bacillus sp. DS11 was cloned in Escherichia coli and its sequence determined. A 560-bp DNA fragment was used as a probe to screen the genomic library. It was obtained through PCR of Bacillus sp. DS11 chromosomal DNA and two oligonucleotide primers based on Nterminal amino acid sequences of the pur… Show more

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Cited by 57 publications
(77 citation statements)
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“…Muy pocas fitasas han sido descritas como altamente específicas para el ácido fítico, entre ellas se encuentran las fitasas alcalinas del género Bacillus. La clonación de genes que codifican fitasas alcalinas de diferentes especies de Bacillus han sido reportadas por diferentes autores (Kim et al 1998, Kerovuo et al 1998 por su actividad a pH neutro, elevada termoestabilidad y gran afinidad por el ácido fítico (Tye et al 2002).…”
Section: Introductionunclassified
“…Muy pocas fitasas han sido descritas como altamente específicas para el ácido fítico, entre ellas se encuentran las fitasas alcalinas del género Bacillus. La clonación de genes que codifican fitasas alcalinas de diferentes especies de Bacillus han sido reportadas por diferentes autores (Kim et al 1998, Kerovuo et al 1998 por su actividad a pH neutro, elevada termoestabilidad y gran afinidad por el ácido fítico (Tye et al 2002).…”
Section: Introductionunclassified
“…amyloliquefaciens DS11 producing a thermostable DS11 phytase was isolated from the soil of a Korean cattle shed. An extracelluar DS11 phytase from the bacterium was purified and biochemically characterized [5,6]. …”
mentioning
confidence: 99%
“…amyloliquefaciens DS11 producing a thermostable DS11 phytase was isolated from the soil of a Korean cattle shed. An extracelluar DS11 phytase from the bacterium was purified and biochemically characterized [5,6] The availability of phosphorus in most animal feedstuff of plant origin is quite low due to phytic acid, which does not get digested by the endogenous enzymes in monogastric animals. Phytic acid (inositolhexaphosphoric acid) is a sixcarbon inositol ring compound with six phosphate group and is the major phosphate storage compound in the seeds of higher plants such as cereals and legumes [7].…”
mentioning
confidence: 99%
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