Cloning of Wap65 in sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) and expression in sea bass tissues

Pierre, Sandra; Coupé, Stéphane; d'Alvise, Nathalie Prévot; Gaillard, Sylvain; Richard, Stéphane; Gouze, Elvire; Aubert, Jacques; Grillasca, Joël-Paul

doi:10.1016/j.cbpb.2010.01.002

Cited by 16 publications

(12 citation statements)

References 24 publications

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“…7B). This protein is thought to be involved in the warm temperature acclimatory response (Kikuchi et al, 1995) and the heat stress response in fish (Picard and Schulte, 2004;Pierre et al, 2010;Choi, 2010). In contrast to previous studies, WAP-65 increased with 9°C acclimation in G. mirabilis, suggesting that this protein may play a role in thermal acclimation regardless of the temperature and that its role might vary depending on the organism.…”

Section: Iron Homeostasis Antioxidant Response and Hypoxic Injurymentioning

confidence: 56%

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Jayasundara

Tomanek

Dowd

et al. 2015

Journal of Experimental Biology

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Cardiac function is thought to play a central role in determining thermal optima and tolerance limits in teleost fishes. Investigating proteomic responses to temperature in cardiac tissues may provide insights into mechanisms supporting the thermal plasticity of cardiac function. Here, we utilized a global proteomic analysis to investigate changes in cardiac protein abundance in response to temperature acclimation (transfer from 13°C to 9, 19 and 26°C) in a eurythermal goby, Gillichthys mirabilis. Proteomic data revealed 122 differentially expressed proteins across acclimation groups, 37 of which were identified using tandem mass-spectrometry. These 37 proteins are involved in energy metabolism, mitochondrial regulation, iron homeostasis, cytoprotection against hypoxia, and cytoskeletal organization. Compared with the 9 and 26°C groups, proteins involved in energy metabolism increased in 19°C-acclimated fish, indicating an overall increase in the capacity for ATP production. Creatine kinase abundance increased in 9°C-acclimated fish, suggesting an important role for the phosphocreatine energy shuttle in cold-acclimated hearts. Both 9 and 26°C fish also increased abundance of hexosaminidase, a protein directly involved in posthypoxia stress cytoprotection of cardiac tissues. Cytoskeletal restructuring appears to occur in all acclimation groups; however, the most prominent effect was detected in 26°C-acclimated fish, which exhibited significantly increased actin levels. Overall, proteomic analysis of cardiac tissue suggests that the capacity to adjust ATP-generating processes is crucial to the thermal plasticity of cardiac function. Furthermore, G. mirabilis may optimize cellular functions at temperatures near 19°C, which lies within the species' preferred temperature range.

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Section: Iron Homeostasis Antioxidant Response and Hypoxic Injurymentioning

confidence: 56%

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Jayasundara

Tomanek

Dowd

et al. 2015

Journal of Experimental Biology

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show abstract

“…Therefore, the tissue expression patterns of the Wap65 isoforms under normal conditions differ depending on the species. However, it should also be noted that the tissue expression patterns of the Wap65 transcripts are altered by the environmental temperature, as previously observed in the sea bass (Dicentrarchus labrax; Perciformes), in which the liver-exclusive expression of Wap65-1 mRNA at low temperatures is markedly changed to a more ubiquitous distribution in various tissues when the environmental temperature is elevated [4].…”

Section: Tissue Distribution and Developmental Expression Of Mlwap65 mentioning

confidence: 83%

“…Fish are poikilothermic animals and their physiology is fundamentally affected by environmental temperatures. For this reason, their acclimation response is a pivotal reaction in maintaining their homeostasis and/or their optimal performance under new environmental conditions [3,4]. Hence, the Wap65 protein is important for our understanding of the molecular mechanisms that underlie the thermal acclimation of eurythermal fish.…”

Section: Introductionmentioning

confidence: 99%

Modulation of warm-temperature-acclimation-associated 65-kDa protein genes (Wap65-1 and Wap65-2) in mud loach (Misgurnus mizolepis, Cypriniformes) liver in response to different stimulatory treatments

Cho

Kim

et al. 2012

Fish & Shellfish Immunology

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“…Wap65, originally found in goldfish [41], is involved in warm acclimation physiological responses [42]. Recently, it has been reported that in fish Wap65 may also be involved in the immune response [43], therefore the decrease of Wap65 in Cu-exposed fish could result in impaired immune response.…”

Section: Differentially Expressed Proteins After Cu Exposurementioning

confidence: 99%

Effects of waterborne Cu exposure in gilthead sea bream (Sparus aurata): A proteomic approach

Isani

Andreani

Carpenè

et al. 2011

Fish & Shellfish Immunology

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Cloning of Wap65 in sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) and expression in sea bass tissues

Cited by 16 publications

References 24 publications

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Modulation of warm-temperature-acclimation-associated 65-kDa protein genes (Wap65-1 and Wap65-2) in mud loach (Misgurnus mizolepis, Cypriniformes) liver in response to different stimulatory treatments

Effects of waterborne Cu exposure in gilthead sea bream (Sparus aurata): A proteomic approach

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