Cloning of zebrafish (Danio rerio) heat shock factor 2 (HSF2) and similar patterns of HSF2 and HSF1 mRNA expression in brain tissues

Yeh, Fu-Lung; Hsu, Lung-Ying; Lin, Bo-An; Chen, Chiao-Fu; Li, I-Chen; Tsai, Shin-Hua; Hsu, Todd

doi:10.1016/j.biochi.2006.07.005

Cited by 16 publications

(16 citation statements)

References 22 publications

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“…From studies on mammals the role of HSF2 is known to be critical during embryonic development and cellular differentiation (Murphy et al 1994;Mezger et al 1994;Pirkkala et al 2001;Wang et al 2003) but the importance of HSF2 in fish still remains to be resolved. However, the fact that hsf2 transcripts were detected at different developmental stages in zebrafish brain (Yeh et al 2006) may indicate that HSF2 performs similar roles in both mammals and fish.…”

Section: Importance Of Hsp During Embryogenesis and Larval Developmentmentioning

confidence: 95%

Advances and perspectives on the regulation and expression of piscine heat shock proteins

Deane

Woo

2010

Rev Fish Biol Fisheries

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The focus of this review is on the regulation and expression of heat shock proteins (HSPs), in fish. Within the past decade, molecular studies pertaining to the isolation and characterization of fish HSP genes have been rapidly expanding with many gene sequences from many fish species being reported. Most of the gene sequences presently available belong to the HSP70 family but genes encoding constitutive and inducible members of the HSP90 family have also been identified as well as genes encoding HSP60, HSP47 and small HSP families. Environmental stressors such as alterations in environmental salinity, disease and chemical exposure are known to alter HSP expression and the regulation of HSPs by hormones has received much attention recently. Heat shock proteins are known to play key roles during embryonic development and recent findings have defined their ontogenetic profiles following hatching. Finally, we are gradually starting to add to our understanding as to the transcriptional regulation of HSPs especially the role and importance of the heat shock factor (HSF).

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Section: Importance Of Hsp During Embryogenesis and Larval Developmentmentioning

confidence: 95%

Advances and perspectives on the regulation and expression of piscine heat shock proteins

Deane

Woo

2010

Rev Fish Biol Fisheries

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“…NM_001013317), corresponds to Mammalian Genome Collection (MGC)/ZGC:113344, and is referenced in the ZFIN library as ZDB-GENE-050306-18 (Sprague et al 2006). Sequence analysis of the cloned cDNA reveals a significant homology to previously characterized zebrafish HSF1 and HSF2 nucleotide sequences (Råbergh et al 2000;Yeh et al 2006) and to mammalian HSF4. The identified mRNA sequence is encoded by a single hsf4 gene that has been mapped to zebrafish chromosome 18, with the genomic sequence spanning 7,103 bp and consisting of eight exons and seven introns.…”

Section: Identification Of a Novel Zebrafish Hsf4 Protein Sequencementioning

confidence: 89%

“…The zebrafish genome contains at least three heat-shock factors; two isoforms of hsf1 (Råbergh et al 2000), hsf2 (Yeh et al 2006), and hsf4. Recent studies by our group have shown that regulation of the hsp70 gene expression by HSF1 is important for development of the zebrafish eye (Krone et al 2003;Evans et al , 2007.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish HSF4: a novel protein that shares features of both HSF1 and HSF4 of mammals

Swan

Evans

Sylvain

et al. 2012

Cell Stress and Chaperones

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Heat-shock proteins (hsps) have important roles in the development of the eye lens. We previously demonstrated that knockdown of hsp70 gene expression using morpholino antisense technology resulted in an altered lens phenotype in zebrafish embryos. A less severe phenotype was seen with knockdown of heat-shock factor 1 (HSF1), suggesting that, while it likely plays a role in hsp70 regulation during lens formation, other regulatory factors are also involved. Heatshock factor 4 plays an important role in mammalian lens development, and an expressed sequence tag encoding zebrafish HSF4 has been identified. The deduced amino acid sequence shares structural similarities with mammalian HSF4 including the lack of an HR-C domain. However, the HR-C domain is absent due to a severe C-terminal truncation within zebrafish HSF4 (zHSF4) relative to the mammalian protein. Surprisingly, the amino acid composition of the zHSF4 DNA binding domain shares a greater degree of identity with HSF1 proteins than it does with mammalian HSF4 proteins. Consistent with this, the binding affinity of in vitro synthesized zHSF4 for discontinuous heat-shock response element sequences is more limited, similar to what has been previously observed for HSF1 proteins. Hsf4 mRNA is expressed in zebrafish adult eye tissue but is only observed in developing embryonic tissue at 60 h post-fertilization or later. This, together with the lack of an observable phenotype following morpholino-based antisense knockdown of hsf4, suggests that zHSF4 is unlikely to play a role in regulating early embryonic lens development.

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“…4). The binding of heat shock factors (HSF) initiates the transcriptional activation of heat shock protein (HSP) genes to heat shock elements located at the promoter regions (Yeh et al 2006). E47 is a basic helix-loop-helix (bHLH) transcription factor that plays an essential role in regulatory cascades such as neuronal differentiation (Guillemot et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms within promoter of Japanese flounder (Paralichthys olivaceus) ovary cytochrome P450-c19 (CYP19a) gene associated with reproductive traits

He¹,

Wen²,

Dong³

et al. 2008

Fish Physiol Biochem

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Cytochrome P450 aromatase, which is encoded by the CYP19a gene, converts androgens to estradiol. Considerable evidence suggests that estrogens play an important role in fish reproductive process. Therefore CYP19a is an excellent candidate gene for reproductive traits. Variants in the promoter of the CYP19a gene might also be involved in the control of aromatase expression and affect regulatory mechanism linking cholesterol metabolism to the synthesis of sex steroids. In this study, nine single-nucleotide polymorphisms (SNPs) were detected with polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP), namely A-680G, G-672A, AGTAGT-649 inserting or deleting, T-623C, C-410A, T7-454A, T-402C, TTTCCAGACTGA-345 inserting or deleting, and G-297C. Nine SNPs within the promoter of the CYP19a gene were tested for association with four reproductive traits [serum testosterone (T), serum 17beta-estradiol (E(2)), hepatosomatic index (HSI), and gonadosomatic index (GSI)] in a population of 50 female Japanese flounder individuals. A locus, P3 (TTTCCAGACTGA-345 inserting or deleting, G-297C), was significantly associated with 17beta-estradiol (E(2)) level (P < 0.05) in female Japanese flounder. In addition, there was significant association between one diplotype based on nine SNPs and reproductive trait. The genetic effect for E(2) level of diplotype D3 was significantly higher than those of other diplotypes (P < 0.05). Results indicate that these genetic effects of those variants on E(2) level may help to explain CYP19a gene status in the reproductive endocrinology of Japanese flounder.

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Cloning of zebrafish (Danio rerio) heat shock factor 2 (HSF2) and similar patterns of HSF2 and HSF1 mRNA expression in brain tissues

Cited by 16 publications

References 22 publications

Advances and perspectives on the regulation and expression of piscine heat shock proteins

Advances and perspectives on the regulation and expression of piscine heat shock proteins

Zebrafish HSF4: a novel protein that shares features of both HSF1 and HSF4 of mammals

Polymorphisms within promoter of Japanese flounder (Paralichthys olivaceus) ovary cytochrome P450-c19 (CYP19a) gene associated with reproductive traits

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