Cloning, Prokaryotic Soluble Expression, and Analysis of Antiviral Activity of Two Novel Feline IFN-ω Proteins

Wang, Xiaona; Li, Fengsai; Han, Meijing; Jia, Shijie; Wang, Li; Qiao, Xinyuan; Jin, Yanping; Cui, Wen; Tang, Lijie; Li, Yijing; Xu, Yigang

doi:10.3390/v12030335

Cited by 11 publications

(10 citation statements)

References 52 publications

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“…Protein expression system includes prokaryotic and eukaryotic systems. Although prokaryotic expression system is most commonly used [ 18 ], the activity of expressed proteins is not good. For example, using prokaryotic system to produce interferon, it is necessary to extract the inclusion bodies in cells to obtain the protein of interest, followed by denaturation, refolding, conformational change in vitro , which is time-consuming and laborious, increases the production cost, and also leads to serious protein loss with some biological activity loss [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, effective therapeutic agents for cat viral diseases are highly desirable. IFN-ω, first reported in 1985 [ 15 ], is secreted primarily by leukocytes, which has now been found in humans [ 16 ] and some animals including cats [ 17 , 18 ], pigs [ 19 ], and horses [ 20 ], etc. IFN-ω combines with IFN receptor complex and activates phosphatidylinositol-3-kinase/protein kinase B (P13K/Akt) signal pathway to exert antiviral activity, thereby achieving the effect of inhibiting viruses [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Cloning and codon optimization of a novel feline interferon omega gene for production by Pichia pastoris and its antiviral efficacy in polyethylene glycol-modified form

Wang

Jiang

et al. 2022

Virulence

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Feline viral diseases, such as feline panleukopenia, feline infectious peritonitis, and feline coronaviral enteritis, seriously endanger the health of cats, and restrict the development of pet industry. Meanwhile, there is a current lack of effective vaccines to protect against feline viral diseases. Thus, effective therapeutic agents are highly desirable. Interferons (IFNs) are important mediators of the antiviral host defense in animals, particularly type I IFNs. In this study, a novel feline IFN omega (feIFN-ω) gene was extracted from the cat stimulated with feline parvovirus (FPV) combined with poly(I:C), and following codon optimization encoding the feIFN-ω, the desired gene (feIFN-ω’) fragment was inserted into plasmid pPICZαA, and transformed into Pichia pastoris GS115, generating a recombinant P. pastoris GS115 strain expressing the feIFN-ω’. After induction, we found that the expression level of the feIFN-ω’ was two times more than that of feIFN-ω ( p < 0.01). Subsequently, the feIFN-ω’ was purified and modified with polyethylene glycol, and its antiviral efficacy was evaluated in vitro and in vivo , using vesicular stomatitis virus (VSV) and FPV as model virus. Our results clearly demonstrated that the feIFN-ω’ had significant antiviral activities on both homologous and heterologous animal cells in vitro . Importantly, the feIFN-ω’ can effectively promote the expression of antiviral proteins IFIT3, ISG15, Mx1, and ISG56, and further enhance host defense to eliminate FPV infection in vivo , suggesting a potential candidate for the development of therapeutic agent against feline viral diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cloning and codon optimization of a novel feline interferon omega gene for production by Pichia pastoris and its antiviral efficacy in polyethylene glycol-modified form

Wang

Jiang

et al. 2022

Virulence

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“…The genome sequence of the BRSV strain and the sequences of its genes encoding proteins G, F, N, NS1, NS2, and SH were deposited in GenBank under accession numbers MT861050, MN316655, MN316656, MN316653, MN316651, MN316652, and MN316654, respectively. Then, for the genes, G, F, N, NS1, NS2 , and SH , cognate sequences from reference BRSV strains representing different subgroups (listed in Table 2 ) were retrieved from the GenBank database by means of the BLAST engine, and phylogenetic analysis was performed on the genes G, F, N, NS1, NS2 , and SH by the neighbor-joining method (1000 replicates) using MEGA 7 software [ 21 ]. After that, alignments of amino acid sequences based on the G gene of the BRSV strain identified in this study and other reference BRSV strains from different subgroups were conducted using the DNAMAN software [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation, identification, and phylogenetic analysis of subgroup III strain of bovine respiratory syncytial virus contributed to outbreak of acute respiratory disease among cattle in Northeast China

et al. 2021

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Bovine respiratory syncytial virus (BRSV) is a clinically important causative agent of acute respiratory diseases in postweaning calves and feedlot cattle and causes numerous economic losses to the cattle industry. In June 2018, an outbreak of an acute respiratory disease occurred among 4- to 10-month-old calves on three intensive beef cattle farms in Heilongjiang Province, Northeast China, with a 27.42% morbidity rate (329/1200) and a > 25% mortality rate (85/329). Using next-generation sequencing, we comprehensively analyzed microbial diversity in the lung samples of the diseased cattle and found that the causative agent of this epidemic outbreak is mainly a bovine orthopneumovirus named BRSV strain DQ. We then isolated and confirmed the virus by RT-PCR and an indirect immunofluorescence assay. Phylogenetic analysis of genes G, F, N, NS1, NS2 , and SH of BRSV strain DQ showed that this strain shares the highest genetic similarity with strains USII/S1, 15489, V41, and NY487834 belonging to subgroup III of BRSV. This is the first report of subgroup III strain of BRSV presence in China. Heilongjiang Province is a major cattle-breeding province in China; therefore, it is necessary to test for BRSV in the cattle trade and to conduct region-extended epidemiological surveillance for BRSV in China.

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“…For FIP, treatment is focused on reducing the inflammatory and hyperimmune response, with several anti-FCoV agents and immunosuppressants also considered. Drugs that target FCoVs include carbohydrate-binding agents ( Galanthus nivalis agglutinin) [ 96 , 97 , 98 ], HIV protease inhibitors (nelfinavir) [ 96 ], interferons [ 99 , 100 , 101 , 102 , 103 , 104 , 105 ], nucleoside analogues (ribavirin and GS-441524) [ 106 , 107 ], anti-malaria drugs (chloroquine) [ 108 ], anti-fungal drugs (itraconazole) [ 109 ], immunosuppressants (cyclosporin A) [ 110 , 111 ], 3C-like proteases (GC376) [ 112 , 113 ], and peptides of heptad repeats of S protein of FCoV [ 114 ]. However, side effects should be considered, as many of these drugs have not been systematically tested in animals.…”

Section: Treatmentmentioning

confidence: 99%

Animal Coronavirus Diseases: Parallels with COVID-19 in Humans

Lin

Chan

Ooi

et al. 2021

Viruses

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Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus in humans, has expanded globally over the past year. COVID-19 remains an important subject of intensive research owing to its huge impact on economic and public health globally. Based on historical archives, the first coronavirus-related disease recorded was possibly animal-related, a case of feline infectious peritonitis described as early as 1912. Despite over a century of documented coronaviruses in animals, the global animal industry still suffers from outbreaks. Knowledge and experience handling animal coronaviruses provide a valuable tool to complement our understanding of the ongoing COVID-19 pandemic. In this review, we present an overview of coronaviruses, clinical signs, COVID-19 in animals, genome organization and recombination, immunopathogenesis, transmission, viral shedding, diagnosis, treatment, and prevention. By drawing parallels between COVID-19 in animals and humans, we provide perspectives on the pathophysiological mechanisms by which coronaviruses cause diseases in both animals and humans, providing a critical basis for the development of effective vaccines and therapeutics against these deadly viruses.

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Cloning, Prokaryotic Soluble Expression, and Analysis of Antiviral Activity of Two Novel Feline IFN-ω Proteins

Cited by 11 publications

References 52 publications

Cloning and codon optimization of a novel feline interferon omega gene for production by Pichia pastoris and its antiviral efficacy in polyethylene glycol-modified form

Cloning and codon optimization of a novel feline interferon omega gene for production by Pichia pastoris and its antiviral efficacy in polyethylene glycol-modified form

Isolation, identification, and phylogenetic analysis of subgroup III strain of bovine respiratory syncytial virus contributed to outbreak of acute respiratory disease among cattle in Northeast China

Animal Coronavirus Diseases: Parallels with COVID-19 in Humans

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