2006
DOI: 10.1111/j.1574-6968.2006.00373.x
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Cloning, sequence analysis and heterologous expression of theMyrothecium gramineumorotidine-5′-monophosphate decarboxylase gene

Abstract: A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide sequence and the amino acid sequence were compared with fungal OMPD sequences. They show the highest similarity to OMPD genes and enzymes of Aspergillus sp., Penicillium sp. and Cladosporium fulvum… Show more

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Cited by 12 publications
(13 citation statements)
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“…The primers CYP52gwF and CYP52gwR (Table S1) were therefore designed for the genome walking PCR to amplify the DNA upstream and downstream of the amplified region of 525 bp. The PCR reaction mixture and cycles were optimized for use with the Expand Long Template PCR System as described by De Maeseneire et al (2006). PCR products were sequenced on both strands using T7 and SP6 primers on an ABI 310 Genetic Analyzer (Applied Biosystems) using Big Dye Terminator kit (Applied Biosystems).…”
Section: Genome Walking and Sequencing Of S Cerevisiae Cyp52a Genementioning
confidence: 99%
“…The primers CYP52gwF and CYP52gwR (Table S1) were therefore designed for the genome walking PCR to amplify the DNA upstream and downstream of the amplified region of 525 bp. The PCR reaction mixture and cycles were optimized for use with the Expand Long Template PCR System as described by De Maeseneire et al (2006). PCR products were sequenced on both strands using T7 and SP6 primers on an ABI 310 Genetic Analyzer (Applied Biosystems) using Big Dye Terminator kit (Applied Biosystems).…”
Section: Genome Walking and Sequencing Of S Cerevisiae Cyp52a Genementioning
confidence: 99%
“…Four gene-speciWc primers (GSP) were designed and are listed in Table 1. The PCR reaction mixture and cycles were optimized for use with the Expand Long Template PCR System (Roche Diagnostics), as described by De Maeseneire et al [14].…”
Section: Genome Walkingmentioning
confidence: 99%
“…mQ-water was added up to 50 ll. The primers (Sigma Genosys) were 'forwardM < 1kb' and 'reverseM < 1kb' (5 0 -ATCCGAG CCACAACGAATCC-3 0 and 5 0 -AATCAGAGCCGC CACGTATG-3 0 , based on the M. gramineum orotidine monophosphate decarboxylase gene (De Maeseneire et al 2006) for PCR1 and 'forwardM > 1kb' and 'reverseM > 1kb' (5 0 -CAGACCGCATGCATTCGT ATGCC-3 0 and 5 0 -ACCAAGCACCAACAC-3 0 , based on the flanking sequences of the M. gramineum ompd) for PCR2 on genomic DNA of M. gramineum.…”
Section: Amplification Of 'Small' Fragmentsmentioning
confidence: 99%
“…Myrothecium gramineum is an Ascomycete used in ongoing research as a new expression host (Jonniaux et al 2004;De Maeseneire et al 2006). The Aspergillus strain was used as a reference.…”
Section: Introductionmentioning
confidence: 99%