Cloning, tissue distribution and sub-cellular localisation of phospholipase C X-domain containing protein (PLCXD) isoforms

Gellatly, Steven; Kalujnaia, Svetlana; Cramb, Gordon

doi:10.1016/j.bbrc.2012.06.079

Cited by 26 publications

(36 citation statements)

References 14 publications

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“…Plcxd3 encodes a protein about which relatively little is known. It has catalytic activity against phosphatidylinositol, is expressed abundantly in neural tissues, and when overexpressed in cultured cell lines, locates to cytoplasmic organelles (Gellatly et al 2012). In contrast, the functions of the proteins encoded by the other newly identified activity-dependent mRNAs add further support to evidence that odor stimulation tends to regulate mRNAs encoding proteins with roles in axonal or synaptic function and maintenance and in calcium regulation of as yet undefined biochemical events (Imai et al 2009;Bennett et al 2010).…”

Section: Activity-dependent Mrnas Encoding Axonal and Calciumbinding mentioning

confidence: 79%

Activity-Dependent Genes in Mouse Olfactory Sensory Neurons

et al. 2014

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Activity-dependent survival of olfactory sensory neurons (OSNs) may allow animals to tune their olfactory systems to match their odor environment. Activity-dependent genes should play important roles in this process, motivating experiments to identify them. Both unilateral naris occlusion of mice for 6 days and genetic silencing of OSNs decreased S100A5, Lrrc3b, Kirrel2, Slc17a6, Rasgrp4, Pcp4l1, Plcxd3, and Kcnn2 while increasing Kirrel3. Naris occlusion also decreased Eml5, Ptprn, and Nphs1. OSN number was unchanged and stress-response mRNAs were unaffected after 6 days of naris occlusion. This leaves odor stimulation as the most likely cause of differential abundance of these mRNAs, but through a mechanism that is slow or indirect for most because 30-40 min of odor stimulation increased only 3 of 11 mRNAs decreased by naris occlusion: S100A5, Lrrc3b, and Kirrel2. Odorant receptor (OR) mRNAs were significantly more variable than the average mRNA, consistent with difficulty in reliably detecting changes in these mRNAs after 6 days of naris occlusion. One OR mRNA, Olfr855, was consistently decreased, however. These results suggest that the latency from the cessation of odor stimulation to effects on activity-dependent OSN survival must be a week or more in juvenile mice.

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Section: Activity-dependent Mrnas Encoding Axonal and Calciumbinding mentioning

confidence: 79%

Activity-Dependent Genes in Mouse Olfactory Sensory Neurons

et al. 2014

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“…The DNF2 atypical structure makes difficult to predict its biochemical function despite that bacterial PI-PLCs containing only the X-domain were shown to be functional and to be able to cleave phosphatidyl-inositol and/or GPI anchors [53]. A recent study suggests that human PI-PLCXD containing protein also displays phospholipase activity while laking the Y and Ca 2+ binding domains [54], leaving open the possibility that PI-PLCXD containing proteins, amongst which is DNF2, play a role in phospholipid cleavage. Without any demonstrated biochemical activity, for now, it is only possible to speculate why DNF2 is unnecessary under permissive condition.…”

Section: Discussionmentioning

confidence: 99%

Growth Conditions Determine the DNF2 Requirement for Symbiosis

et al. 2014

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Rhizobia and legumes are able to interact in a symbiotic way leading to the development of root nodules. Within nodules, rhizobia fix nitrogen for the benefit of the plant. These interactions are efficient because spectacularly high densities of nitrogen fixing rhizobia are maintained in the plant cells. DNF2, a Medicago truncatula gene has been described as required for nitrogen fixation, bacteroid’s persistence and to prevent defense-like reactions in the nodules. This manuscript shows that a Rhizobium mutant unable to differentiate is not sufficient to trigger defense-like reactions in this organ. Furthermore, we show that the requirement of DNF2 for effective symbiosis can be overcome by permissive growth conditions. The dnf2 knockout mutants grown in vitro on agarose or Phytagel as gelling agents are able to produce nodules fixing nitrogen with the same efficiency as the wild-type. However, when agarose medium is supplemented with the plant defense elicitor ulvan, the dnf2 mutant recovers the fix− phenotype. Together, our data show that plant growth conditions impact the gene requirement for symbiotic nitrogen fixation and suggest that they influence the symbiotic suppression of defense reactions in nodules.

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“…These are proposed to have distinct functions due to their varied tissue distribution and cellular location but are all likely to be active phosphodiesterases as they increase the turnover of inositol phosphate (InsP) [19]. In a cell culture model PLCXD3 was found in cytoplasmic and perinuclear vesicles and from analysis of RNA levels it was expressed in a wide range of tissue types suggesting a potential key cellular role, with predominant expression seen in the brain [19]. The role of PLCXD3 in neurodegenerative disease is as yet unknown, although PI-PLC and specifically Ca 2+ homeostasis have been associated with neurodegeneration.…”

Section: Discussionmentioning

confidence: 99%

Splice site SNPs of phospholipase PLCXD3 are significantly associated with variant and sporadic Creutzfeldt-Jakob disease

2013

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BackgroundVariant Creutzfeldt-Jakob disease is an infectious, neurodegenerative, protein-misfolding disease, of the prion disease family, originally acquired through ingestion of meat products contaminated with bovine spongiform encephalopathy (BSE). Public health concern was increased by the discovery of human-to-human transmission via blood transfusion. This study has verified a novel genetic marker linked to disease risk.MethodsSNP imputation and association testing indicated those genes that had significant linkage to disease risk and one gene was investigated further with Sanger resequencing. Results from variant Creutzfeldt-Jakob disease were compared with those from sporadic (idiopathic) Creutzfeldt-Jakob disease and published controls.ResultsThe most significant disease risk, in addition to the prion protein gene, was for the phosphatidylinositol-specific phospholipase C, X domain containing 3 (PLCXD3) gene. Sanger resequencing of CJD patients across a region of PLCXD3 with known variants confirmed three SNPs associated with variant and sporadic CJD.ConclusionsThese data provide the first highly significant confirmation of SNP allele frequencies for a novel CJD candidate gene providing new avenues for investigating these neurodegenerative prion diseases. The phospholipase PLCXD3 is primarily expressed in the brain and is associated with lipid catabolism and signal transduction.

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Cloning, tissue distribution and sub-cellular localisation of phospholipase C X-domain containing protein (PLCXD) isoforms

Cited by 26 publications

References 14 publications

Activity-Dependent Genes in Mouse Olfactory Sensory Neurons

Activity-Dependent Genes in Mouse Olfactory Sensory Neurons

Growth Conditions Determine the DNF2 Requirement for Symbiosis

Splice site SNPs of phospholipase PLCXD3 are significantly associated with variant and sporadic Creutzfeldt-Jakob disease

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