Cloning two P5CS genes from bioenergy sorghum and their expression profiles under abiotic stresses and MeJA treatment

Su, Man; Li, Xiaofeng; Ma, Xiaojun; Peng, Xianjun; Zhao, An Ping; Cheng, Long; Chen, Shuang-Yan; Liu, Gongshe

doi:10.1016/j.plantsci.2011.03.002

Cited by 103 publications

(53 citation statements)

References 30 publications

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“…It suggested that AOS might involve in ABA signaling pathway by stimulating ABA synthesis to induce expression of drought resistance genes and to improve capacity of drought resistance. In higher plants, P5CS gene, which is one of the key genes for proline synthesis from glutamate and ornithine, could be induced and up-regulated by drought stress [35,36]. Our results showed that P5CS in wheat leaves was induced and up-regulated by exogenous AOS under drought condition.…”

Section: Discussionmentioning

confidence: 62%

Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress

Liu

Yun-hong

Yin

et al. 2013

Plant Physiology and Biochemistry

112

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Section: Discussionmentioning

confidence: 62%

Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress

Liu

Yun-hong

Yin

et al. 2013

Plant Physiology and Biochemistry

112

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“…P5CS genes have since been cloned from A. thaliana [43], Medicago sativa [44], Lycopersicon esculentum [45], Medicago truncatula [46] and other species [47][48][49][50]. P5CS genes have been extensively studied in glycophytes that apparently lack the Pro tolerance mechanism; however, only a small number of studies have been done in halophytes [51].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Zheng

Dang

et al. 2013

Mol Biol Rep

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Several functional and regulatory proteins play important roles in controlling plant stress tolerance. Proline (Pro) is one of the most accumulated osmolytes correlated with tolerance to stresses. Δ(1)-Pyrroline-5-carboxylate synthetase (P5CS) is a rate-limiting enzyme in Pro biosynthesis. In the present study, we isolated the cDNA for a P5CS gene (NtP5CS) from the halophyte Nitraria tangutorum. Phylogenetic analysis and subcellular localization analysis of NtP5CS-GFP protein in onion cells showed that NtP5CS was a new P5CS gene and was involved in Pro synthesis in N. tangutorum. Expression of the NtP5CS gene was induced by salt stress, dehydration, and high and low temperatures. Escherichia coli overexpressing AtP5CS or NtP5CS exhibited better growth in all treatments, including high salinity, high alkalinity, dehydration, osmotic, heat and cold stresses. Additionally, NtP5CS recombinant E. coli cells grew better than did AtP5CS recombinant cells in response to abiotic stresses. Our data demonstrate that the P5CS from a halophytic species functions more efficiently than its homologue from a glycophytic species in improving the stress tolerance of E. coli.

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“…In rice, P5CS1 and P5CS2 are upregulated by different stresses (Igarashi et al, 1997;Hur et al, 2004). In Sorghum bicolor, Su et al (2011) found that P5CS2 is a housekeeping gene that mainly functions in basic proline metabolism; the P5CS1 gene could play a major role in stress responses. In this study, RNA expression analysis showed that Zm P5CS2 (Pro1) is constitutively expressed and not inducible by drought and acid stresses (Supplemental Figure 6).…”

Section: Pro1 Encodes a P5cs Protein In Maizementioning

confidence: 99%

Retracted: Proline responding1Plays a Critical Role in Regulating General Protein Synthesis and the Cell Cycle in Maize

et al. 2014

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Proline, an important amino acid, accumulates in many plant species. Besides its role in plant cell responses to environmental stresses, the potential biological functions of proline in growth and development are unclear. Here, we report cloning and functional characterization of the maize (Zea mays) classic mutant proline responding1 (pro1) gene. This gene encodes a D 1-pyrroline-5-carboxylate synthetase that catalyzes the biosynthesis of proline from glutamic acid. Loss of function of Pro1 significantly inhibits proline biosynthesis and decreases its accumulation in the pro1 mutant. Proline deficiency results in an increased level of uncharged tRNA pro AGG accumulation and triggers the phosphorylation of eukaryotic initiation factor 2a (eIF2a) in the pro1 mutant, leading to a general reduction in protein synthesis in this mutant. Proline deficiency also downregulates major cyclin genes at the transcriptional level, causing cell cycle arrest and suppression of cell proliferation. These processes are reversible when external proline is supplied to the mutant, suggesting that proline plays a regulatory role in the cell cycle transition. Together, the results demonstrate that proline plays an important role in the regulation of general protein synthesis and the cell cycle transition in plants. INTRODUCTION In maize (Zea mays), a series of seed mutants with a starchy endosperm have been identified, and the cause of the opa-que/floury kernel phenotype has been investigated through analyzing the functions of cloned genes. Previous studies suggested that most of the opaque and floury mutants (opa-que2, floury2, Mucronate, and Defective endosperm B30) involve genes that regulate zein synthesis, such as regulatory genes or structural genes for these storage proteins (Schmidt et al., 1987; Coleman et al., 1995; Gillikin et al., 1997; Kim et al., 2004, 2006). Downregulation of zein gene (a-, b-, d-, and g-zein genes) expression by RNA interference also reproduced an opaque phenotype (Segal et al., 2003; Wu and Messing, 2010). These results indicate that the opaque/floury phenotypes of opaque2 (o2), floury2 (fl2), Defective endo-sperm B30, and Mucronate mutants are caused by either quantitative or qualitative alterations in zein proteins. However , other opaque/floury mutants, such as o1, o5, and fl1, show no notable alterations in zein proteins (Holding et al.

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Cloning two P5CS genes from bioenergy sorghum and their expression profiles under abiotic stresses and MeJA treatment

Cited by 103 publications

References 30 publications

Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress

Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Retracted: Proline responding1Plays a Critical Role in Regulating General Protein Synthesis and the Cell Cycle in Maize

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