Cloning Vectors Based on Cryptic Plasmids Isolated from Lactic Acid Bacteria:Their Characteristics and Potential Applications in Biotechnology

Abstract

Lactic acid bacteria (LAB) are Gram positive bacteria, widely distributed in nature, and industrially important as they are used in a variety of industrial food fermentations. The use of genetic engineering techniques is an effective means of enhancing the industrial applicability of LAB. However, when using genetic engineering technology, safety becomes an essential factor for the application of improved LAB to the food industry. Cloning and expression systems should be derived preferably from LAB cryptic plasmids that generally encode genes for which functions can be proposed, but no phenotypes can be observed. However, some plasmid-encoded functions have been discovered in cryptic plasmids originating from Lactobacillus, Streptococcus thermophilus, and Pediococcus spp. and can be used as selective marker systems in vector construction. This article presents information concerning LAB cryptic plasmids, and their structures, functions, and applications. A total of 134 cryptic plasmids collated are discussed.

Genomics of Weissella cibaria with an examination of its metabolic traits

“…lactis UC317. The single ORF on the smallest plasmid (pKML5), encoding the replication protein, showed 28 % similarity to a replication protein on a plasmid (pFR18) from Leuconostoc mesenteroides that replicates via the rolling-circle mechanism (Shareck et al, 2004). In addition, this small plasmid shares 94 % sequence identity (98 % coverage) with a 1490 bp plasmid, pKLCA, in another W. cibaria strain (Park et al, 2007).…”

“…extra-chromosomal DNA elements that encode no recognizable phenotype besides their replication functions (Shareck et al, 2004)]. In addition to replicative proteins, other ORFs are encoded on plasmids pKML2, pKML3 and pKML4, including a putative oxidoreductase, a xylose/proton symporter and an HNH endonuclease.…”

pRS4: UN VECTOR DE CLONACIÓN IDÓNEO PARA BACTERIAS ÁCIDO-LÁCTICAS DE USO ALIMENTARIO pRS4: AN APPROPRIATE CLONING VECTOR FOR LACTIC-ACID BACTERIA OF FOOD USE

“…Una vía potencial de manipulación genética de las BAL de uso alimentario es la utilización de vectores plasmídicos de clonación que permitan la introducción de nuevos genes (Geis, 2003;Shareck et al, 2004), pero esta vía conlleva la posibilidad de transferencia horizontal de SOMENTA CIENCIA Y TECNOLOGÍA ALIMENTARIA Sociedad Mexicana de Nutrición y Tecnología de los Alimentos plásmidos entre bacterias coexistentes en un mismo alimento (De las Rivas et al, 2004;Francia et al, 1994;Wang et al, 2006), lo que podría conllevar la adquisición de ventajas ecológicas a bacterias no deseables como E. faecalis, cuya presencia en alimentos puede ser síntoma de falta de higiene o contaminación fecal.…”

The Microbiome of Spodoptera littoralis: Development, Control and Adaptation to the Insect Host

“…The choice of a reliable expression vector depends on several factors, such as the mode of replication, copy number and stability [63]. The expression vector used in this study is derived from pTRKH3 plasmid with a broad host range.…”