Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: mechanisms involved and potential as a biocontrol agent

Abstract

Aims: To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. Methods and Results: The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual cultures. The strain effectively antagonized S. sclerotiorum in pot‐grown lettuce and soybean plants. Antifungal activity assays of C. rosea BAFC3874 grown in culture established that the strain produced antifungal compounds against S. sclerotiorum associated with secondary metabolism. High mycelial growth inhibition coincided with sclerotia production inhibition. The C. rosea strain produced a microheterogeneous mixture of peptides belonging to the peptaibiotic family. Moreover, mycoparasitism activity was observed in the dual culture. Conclusions: Clonostachys rosea strain BAFC3874 was proved to be an effective antagonist against the aggressive soil‐borne pathogen S. sclerotiorum in greenhouse experiments. The main mechanisms involve peptaibiotic metabolite production and mycoparasitism activity. Significance and Impact of the Study: Clonostachys rosea BAFC3874 may be a good fungal biological control agent against S. sclerotiorum. In addition, we were also able to isolate and identify peptaibols, an unusual family of compounds in this genus of fungi.

Isolation and Total Synthesis of Albu­peptins A–D: 11‐Residue Peptaibols from the Fungus Gliocladium album

“…[14] The amphipathic nature and helical secondary structure of these compounds allows them to form voltage-dependent ion channels into biological membranes, leading to alterations in the osmotic balance of the cell. [15][16][17] Consequently, peptaibols show a wide range the isolated peptaibols 1-4 was unambiguously assigned by 1 H NMR chemical shift analysis in conjunction with solidphase peptide synthesis. By using this approach, the absolute configuration of the Iva residues in albupeptins A (1) and C (3) was determined to be D, whereas albupeptins B (2) and D (4) feature an additional Iva 5 residue with an L configuration.…”

Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum

“…Several mechanisms are involved in the biocontrol process of C. rosea , including mycoparasitism, antagonism, competition for nutrients and space, induction of plant resistance (Lahoz et al 2004; Papavizas 1985; Rodríguez et al 2011), and the secretion of a series of cell wall-degrading enzymes (Carsolio et al 1999; Elad and Kapat 1999; Giczey et al 2001). Of these, extracellular lytic enzymes, especially chitinase, are considered essential for activity against plant pathogens.…”

Antagonism of Trichoderma harzianum and Clonostachys rosea against fungi associated with grapevine decline in Kurdistan region - Iraq

“…This species also produces fungal inhibitors and cell wall degrading enzymes, induce the loss of turgor and cause lyses of pathogenic hyphae (Papavizes, 1985;Sutton et al, 1997). C. rosea showed ability for production chitinase and glucanase enzymes as well as secondary metabolites (peptides) (Xue 2003; Pisi et al 2006;Roberti et al 2008;Chatterton & Punja 2009;Rodríguez et al 2011). It was also reported to grow endophytically in cucumber (Chatterton et al 2008); and to induce expression defense genes in wheat and canola (Roberti et al 2008;Lahlali& Peng 2013).…”