Close linkage of genes encoding glutamine synthetases I and II in Frankia alni CpI1

Hosted, Thomas J.; Rochefort, D A; Benson, David R.

doi:10.1128/jb.175.11.3679-3684.1993

Cited by 21 publications

(15 citation statements)

References 41 publications

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“…From position 120 to 200, the signals were variable being sometimes highly probable or less probable coding sequences. The putative start codon is positioned at nucleotide 178 [24]. Upstream of nucleotide position 120, the DNA sequence does not seem to be a coding sequence.…”

Section: Characterisation Of a Glnii Frankia Library Clonementioning

confidence: 99%

“…Blast searches con¢rmed the presence of a glnII sequence at the T7 end and detected a glnI sequence at the T3 end. Comparisons were performed with the Frankia CpI1 reported glnI [24] and glnII [18] DNA sequences: 93% identity was observed between the glnII regions and 95% between the glnI regions. Analyses of the ends of the sequenced regions showed the presence of a segment of the pRK404 polylinker region at the T7/glnII end.…”

Section: Characterisation Of a Glnii Frankia Library Clonementioning

confidence: 99%

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Analysis of Frankia evolutionary radiation using glnII sequences

Cournoyer

1999

FEMS Microbiology Letters

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Using a glnII (encoding glutamine synthetase II) PCR selective screening, a Frankia ACN14a gene library clone was isolated. A derived glnII-hybridising 2.7-kb HindIII subclone was characterised. Identities of 95% and 93% were observed, respectively, with the corresponding Frankia CpI1 glnI and glnII regions. A variable segment of the glnII region was selected, PCR amplified from various Frankia genomes, sequenced, and used to investigate phylogenetic relationships within the genus. glnII phylogenetic inferences are well-resolved and allowed us to deduce evolutionary trends among Frankia. Frankia radiation seems to begin with a diversification according to the ability or not to infect actinorhizal plants. The infective strains are divided into two clusters matching plant-colonising specificities. ß

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Section: Characterisation Of a Glnii Frankia Library Clonementioning

confidence: 99%

Section: Characterisation Of a Glnii Frankia Library Clonementioning

confidence: 99%

Analysis of Frankia evolutionary radiation using glnII sequences

Cournoyer

1999

FEMS Microbiology Letters

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“…The methods used for plasmid isolation, restriction enzyme digestion, DNA modification and E. coli transformation were all done according to the suppliers' recommendations. Micromonospora genomic DNA was isolated as described previously (Hosted et al, 1993). PCR amplifications were performed using the Advantage GC DNA polymerase and reagents (Clontech) and PCR products were cloned utilizing Topo TA cloning kits (Invitrogen) or the pNOTA vector (59-39).…”

Section: Methodsmentioning

confidence: 99%

Development of the Micromonospora carbonacea var. africana ATCC 39149 bacteriophage pMLP1 integrase for site-specific integration in Micromonospora spp.

Alexander¹,

Devlin²,

Hewitt³

et al. 2003

Microbiology

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Development of the Micromonospora carbonacea var. africana ATCC 39149 bacteriophage pMLP1 integrase for site-specific integration in Micromonospora spp. Micromonospora carbonacea var. africana ATCC 39149 contains a temperate bacteriophage, pMLP1, that is present both as a replicative element and integrated into the chromosome. Sequence analysis of a 4?4 kb KpnI fragment revealed pMLP1 att/int functions consisting of an integrase, an excisionase and the phage attachment site (attP). Plasmids pSPRH840 and pSPRH910, containing the pMLP1 att/int region, were introduced into Micromonospora spp. by conjugation from Escherichia coli. Sequence analysis of DNA flanking the integration site confirmed site-specific integration into a tRNA His gene in the chromosome. The pMLP1 attP element and chromosomal bacterial attachment (attB) site contain a 24 bp region of sequence identity located at the 39 end of the tRNA. Integration of pMLP1-based plasmids in M. carbonacea var. africana caused a loss of the pMLP1 phage. Placement of an additional attB site into the chromosome allowed integration of pSPRH840 into the alternate attB site. Plasmids containing the site-specific att/int functions of pMLP1 can be used to integrate genes into the chromosome.

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“…Cosmid libraries of S. lavendulae IMRU 3455 and IMRU 467 were prepared in pSupercos I according to the manufacturer's recommendations (Stratagene). Cosmid libraries and dot-blot screening filters were prepared as described previously (Hosted et al, 1993). pSLV45 DNA was isolated by excision from a PFGE agarose gel, equilibrated with BamHI digestion buffer, digested with BamHI, ethanol-precipitated and used as a DNA probe against the S. lavendulae IMRU 3455 cosmid library to isolate the pSLV45-containing cosmids pSPRX604 and pSPRX600.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

Hosted¹,

Wang²,

Horan³

2004

Microbiology

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Streptomyces lavendulae IMRU 3455 contains two large linear plasmids designated pSLV45 (45 kb) and pSLV195 (195 kb). A cosmid, pSPRX604, containing 42 kb from pSLV45 was cloned and sequenced. pSLV45 was tagged with a hygromycin-resistance marker by homologous recombination to generate the derivatives pSLV45.680 and pSLV45.681. An apramycin-resistance marker was introduced into S. lavendulae IMRU 467 using the pSPR910 integration vector to yield the recipient strain SPW910. The self-transmissible nature of pSLV45 was determined by transfer of pSLV45.680 and pSLV45.681 from the donor strains SPW680 and SPW681 into the recipient strain SPW910. Southern analysis indicated the presence of hygromycin-and pSLV45-hybridizing sequences within SPW910 exconjugants. PFGE analysis confirmed pSLV45.680 and pSLV45.681 were transferred intact and formed freely replicating linear plasmids. Sequence analysis of pSPRX604 revealed genes predicted to be involved in plasmid transfer, partitioning and regulation. The transfer of the linear plasmid pSLV45 from S. lavendulae IMRU 3455 into S. lavendulae IMRU 467 may allow the development of pSLV45 as an actinomycete-to-actinomycete conjugative shuttle vector. INTRODUCTIONLinear plasmids with 59 terminally attached proteins (TPs) and terminal inverted repeats (TIRs) have been found in bacteria, plants, filamentous fungi and yeasts, and often within actinomycetes (Griffiths, 1995;Hinnebusch & Tilly, 1993;Meinhardt et al., 1990;Rohe et al., 1992). Actinomycete linear plasmids pSLA1 (17 kb) and pSLA2 (17 kb) were first detected in the lankacidin-producer Streptomyces sp. 7434-AN4 and in Streptomyces rochei, respectively (Hayakawa et al., 1979;Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). Both plasmids were shown to contain TIRs and TPs (Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). With the advent of PFGE analysis, other actinomycete linear plasmids have been identified including Streptomyces lividans SLP2 (50 kb), Streptomyces clavuligerus pSCL1 (12 kb), Streptomyces avermitilis pSA1 and pSA2 (100 kb and 250 kb), Streptomyces rimosus pZG101 (387 kb), Streptomyces lasaliensis pKSL (520 kb) and Streptomyces coelicolor SCP1 (363 kb) Evans et al., 1994;Gravius et al., 1994;Keen et al., 1988;Kinashi & Shimaji, 1987;Kinashi & Shimaji-Murayama, 1991;Kinashi et al., 1994). All these plasmids were found to contain TIRs and TPs. In addition, both pSLA2 and SLP2 were shown to contain an internal origin of replication for bi-directional replication of DNA (Chang & Cohen, 1994;Chang et al., 1996;Huang et al., 2003).Unlike the chromosomes of most bacteria, which are circular in nature, the chromosomes of actinomycetes are linear (Lezhava et al., 1995;Lin et al., 1993). This was first demonstrated in S. lividans and has now been established for several other actinomycetes including S. coelicolor, Streptomyces griseus and Saccharopolyspora erythraea (formerly Streptomyces erythraeus) (Lezhava et al., 1995;Lin et al., 1993;Reeves et al., 1998). All actinomycete chromosomes characterized to da...

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Close linkage of genes encoding glutamine synthetases I and II in Frankia alni CpI1

Cited by 21 publications

References 41 publications

Analysis of Frankia evolutionary radiation using glnII sequences

Analysis of Frankia evolutionary radiation using glnII sequences

Development of the Micromonospora carbonacea var. africana ATCC 39149 bacteriophage pMLP1 integrase for site-specific integration in Micromonospora spp.

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

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