Closed Genome Sequence of Escherichia coli K-12 Group Strain C600

Allué-Guardia, Anna; Nyong, Emmanuel C.; Koenig, Scott; Vargas, Sean M.; Bono, James L.; Eppinger, Mark

doi:10.1128/mra.01052-18

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…Conjugation assay was conducted to resolve the EC121 conjugative plasmids. In the mating pair, strain EC121 was the donor strain, whereas E. coli K-12 derived strains, J53 [71] and C600 [72], resistant to sodium azide, were the recipient strains. Conjugation was performed using overnight cultures of the donor and the recipient strains grown in LB, in the proportion of 1:2, respectively.…”

Section: Conjugation Assaymentioning

confidence: 99%

Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

et al. 2020

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Escherichia coli EC121 is a multidrug-resistant (MDR) strain isolated from a bloodstream infection of an inpatient with persistent gastroenteritis and T-zone lymphoma that died due to septic shock. Despite causing an extraintestinal infection, previous studies showed that it did not have the usual characteristics of an extraintestinal pathogenic E. coli. Instead, it belonged to phylogenetic group B1 and harbored few known virulence genes. To evaluate the pathogenic potential of strain EC121, an extensive genome sequencing and in vitro characterization of various pathogenicity-associated properties were performed. The genomic analysis showed that strain EC121 harbors more than 50 complete virulence genetic clusters. It also displays the capacity to adhere to a variety of epithelial cell lineages and invade T24 bladder cells, as well as the ability to form biofilms on abiotic surfaces, and survive the bactericidal serum complement activity. Additionally, EC121 was shown to be virulent in the Galleria mellonella model. Furthermore, EC121 is an MDR strain harboring 14 antimicrobial resistance genes, including blaCTX-M-2. Completing the scenario, it belongs to serotype O154:H25 and to sequence type 101-B1, which has been epidemiologically linked to extraintestinal infections as well as to antimicrobial resistance spread. This study with E. coli strain EC121 shows that clinical isolates considered opportunistic might be true pathogens that go underestimated.

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Section: Conjugation Assaymentioning

confidence: 99%

Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

et al. 2020

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“…However, we note their ability to potentially (re-)acquire Stx through Stx-phage infection (Mellmann et al, 2008). In this study, we applied LRT technology (Koren et al, 2013;Allue-Guardia et al, 2018b) and provide closed genomes and comprehensive phylogenomic analyses for representative Stx (−) NSF O157:H7 and SF O157:NM strains in our collection that tested negative for stx, or were cured of their Stx-phage inventory in our laboratory.…”

Section: Introductionmentioning

confidence: 99%

Pathogenomes of Atypical Non-shigatoxigenic Escherichia coli NSF/SF O157:H7/NM: Comprehensive Phylogenomic Analysis Using Closed Genomes

et al. 2020

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“…To test this hypothesis, we chose three E. coli strains that are useful for different applications. E. coli C600 is a lab strain ( 42 , 43 ), E. coli MG1655 is often used in industrial fermentations ( 44–46 ), and E. coli Nissle 1917 is a probiotic strain ( 5 , 47 , 48 ). We found that phage lysate produced from 10 8 E. coli C600 cells could passage >10 7 variants to E. coli MG1655 and E. coli Nissle 1917 (Figure 4B ).…”

Section: Resultsmentioning

confidence: 99%

Inducible directed evolution of complex phenotypes in bacteria

Al'Abri

Haller

et al. 2022

Nucleic Acids Research

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Directed evolution is a powerful method for engineering biology in the absence of detailed sequence-function relationships. To enable directed evolution of complex phenotypes encoded by multigene pathways, we require large library sizes for DNA sequences >5–10 kb in length, elimination of genomic hitchhiker mutations, and decoupling of diversification and screening steps. To meet these challenges, we developed Inducible Directed Evolution (IDE), which uses a temperate bacteriophage to package large plasmids and transfer them to naive cells after intracellular mutagenesis. To demonstrate IDE, we evolved a 5-gene pathway from Bacillus licheniformis that accelerates tagatose catabolism in Escherichia coli, resulting in clones with 65% shorter lag times during growth on tagatose after only two rounds of evolution. Next, we evolved a 15.4 kb, 10-gene pathway from Bifidobacterium breve UC2003 that aids E. coli’s utilization of melezitose. After three rounds of IDE, we isolated evolved pathways that both reduced lag time by more than 2-fold and enabled 150% higher final optical density. Taken together, this work enhances the capacity and utility of a whole pathway directed evolution approach in E. coli.

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Closed Genome Sequence of Escherichia coli K-12 Group Strain C600

Cited by 8 publications

References 17 publications

Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

Pathogenomes of Atypical Non-shigatoxigenic Escherichia coli NSF/SF O157:H7/NM: Comprehensive Phylogenomic Analysis Using Closed Genomes

Inducible directed evolution of complex phenotypes in bacteria

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