Closed-Tube Barcoding

Sirianni, Nicky M.; Yuan, Huijun; Rice, J. E.; Kaufman, Ronit S.; Deng, Jie; Fulton, Chandler; Wangh, Lawrence J.

doi:10.1139/gen-2016-0026

Cited by 9 publications

(14 citation statements)

References 24 publications

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“…2), which may or may not be contaminated by SN 2014C. To aid the comparison, the HST nativefilter magnitudes in the optical broad bands have been converted to the standard Johnson-Cousins system (F438W and F475X to the B band, F555W to the V band, and F814W to the I band) based on transformation relations reported by Sirianni, et al (2005) and Harris (2018). The UVband and narrow-band magnitudes are kept unchanged as in their native filters.…”

Section: Was the Sn Still Bright?mentioning

confidence: 99%

The changing-type SN 2014C may come from an 11-M⊙ star stripped by binary interaction and violent eruption

Sun

Maund

Crowther

2020

Monthly Notices of the Royal Astronomical Society

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SN 2014C was an unprecedented supernova (SN) that displayed a metamorphosis from Type Ib to Type IIn over ∼200 days. This transformation is consistent with a helium star having exploded in a cavity surrounded by a dense shell of the progenitorâs stripped hydrogen envelope. For at least 5 years post-explosion, the ejecta continued to interact with an outer, extended component of circumstellar medium (CSM) that was ejected even before the dense shell. It is still unclear, however, what kind of progenitor could have undergone such a complicated mass-loss history before it produced this peculiar SN. In this paper, we report a new analysis of SN 2014C’s host star cluster based on data from the Hubble Space Telescope (HST). By carefully fitting its spectral energy distribution (SED), we derive a precise cluster age of 20.0$^{+3.5}_{-2.6}$ Myr, which corresponds to the progenitor’s lifetime assuming coevolution. Combined with binary stellar evolution models, we find that SN 2014C’s progenitor may have been an ∼11-M⊙ star in a relatively wide binary system. The progenitor’s envelope was partially stripped by Case C or Case BC mass transfer via binary interaction, followed by a violent eruption that ejected the last hydrogen layer before terminal explosion. Thus, SN 2014C, in common with SNe 2006jc and 2015G, may be a third example that violent eruptions, with mass-loss rates matching luminous blue variable (LBV) giant eruptions, can also occur in much lower-mass massive stars if their envelopes are partially or completely stripped in interacting binaries.

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Section: Was the Sn Still Bright?mentioning

confidence: 99%

The changing-type SN 2014C may come from an 11-M⊙ star stripped by binary interaction and violent eruption

Sun

Maund

Crowther

2020

Monthly Notices of the Royal Astronomical Society

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“…Whilst it has been known that the COI gene is more slowly evolving in chondrichthyans than teleosts (Moore et al, 2011; Naylor et al, 2012), this is seldom a major issue in most DNA barcoding applications (Fields et al, 2018; Griffiths et al, 2013; Hobbs et al, 2019), so an optimised iteration of the FASTFISH-ID ™ method is poised to be transformational for elasmobranch conservation and management. A qualitative investigation on the full length of COI sequences (Sanger sequencing results) based on visual and simple comparison (https://www.bioinformatics.org/sms2/ident_sim.html) revealed that for those problematic three pairs of species mentioned above for that particular segment, there is a high degree of similarity in their sequence (70-98%), although this seems unlikely as the method is extremely sensitive and easily distinguishes between sequences that differ by a single nucleotide (Sirianni et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

Prasetyo

Cusa

Murray

et al. 2022

Preprint

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Trade restrictions for many endangered elasmobranch species exist to disincentivise their exploitation and curb their declines. However, the variety of products and the complexity of import/export routes make trade monitoring challenging. We investigate the use of a portable, universal, DNA-based tool which would greatly facilitate in-situ monitoring. We collected shark and ray samples across the Island of Java, Indonesia, and selected 28 species (including 22 CITES-listed species) commonly encountered in landing sites and export hubs to test a recently developed real-time PCR single-assay originally developed for screening bony fish. We employed a deep learning algorithm to recognize species based on DNA melt-curve signatures. By combining visual and machine learning assignment methods, we distinguished 25 out of 28 species, 20 of which were CITES-listed. With further refinement, this method can provide a practical tool for monitoring elasmobranch trade worldwide, without the need for a lab or the bespoke design of species-specific assays.

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“…Sirianni et al (2016) outline their unique method termed Closed-Tube Barcoding, which may provide a rapid, affordable alternative to sequencing, especially for projects involving numerous samples. This approach also could be a step towards developing a portable barcoding device, with diverse applications such as monitoring mosquito populations.…”

Section: Advances In Quantificationmentioning

confidence: 99%

From Barcodes to Biomes: Special Issues from the 6th International Barcode of Life Conference

Adamowicz

Chain

Clare

et al. 2016

Genome

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Closed-Tube Barcoding

Cited by 9 publications

References 24 publications

The changing-type SN 2014C may come from an 11-M⊙ star stripped by binary interaction and violent eruption

The changing-type SN 2014C may come from an 11-M⊙ star stripped by binary interaction and violent eruption

Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

From Barcodes to Biomes: Special Issues from the 6th International Barcode of Life Conference

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