2023
DOI: 10.1002/jsfa.12711
|View full text |Cite
|
Sign up to set email alerts
|

Closed‐tube visual detection of Atlantic cod (Gadus morhua) using self‐quenched primer coupled with a designed loop‐mediated isothermal amplification vessel

Abstract: BACKGROUND Species adulteration has been widely revealed around the world, and the possible reasons include declining stocks in most source areas of the world, poor transparency in the global supply chain, and difficulty in distinguishing the features of processed products. The present work selected Atlantic cod (Gadus morhua) as a case study, and developed a novel loop‐mediated isothermal amplification (LAMP) assay for Atlantic cod authentication, where a self‐quenched primer and a newly designed reaction ves… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2023
2023
2025
2025

Publication Types

Select...
5

Relationship

0
5

Authors

Journals

citations
Cited by 9 publications
(1 citation statement)
references
References 34 publications
0
1
0
Order By: Relevance
“…Keeping the dye out of the reaction was important because, at the concentration needed to generate a naked-eye color change, it would have resulted in reaction inhibition ( Oscorbin et al, 2016 ; Quyen et al, 2019 ). It was confirmed that the dye could be added directly to the reaction after the amplification; however, it was discarded as the tubes will have to be opened, and it has been extensively discouraged in the literature due to the very high likelihood of contamination of the laboratory, what will ultimately increase the false positive samples due to this cross-contamination ( Amin Almasi, 2012 ; Chen et al, 2023 ; Hanyue et al, 2023 ; Karthik et al, 2014 ; N. B. Quoc et al, 2021 ; Nguyen Bao Quoc et al, 2018 ).…”
Section: Discussionmentioning
confidence: 99%
“…Keeping the dye out of the reaction was important because, at the concentration needed to generate a naked-eye color change, it would have resulted in reaction inhibition ( Oscorbin et al, 2016 ; Quyen et al, 2019 ). It was confirmed that the dye could be added directly to the reaction after the amplification; however, it was discarded as the tubes will have to be opened, and it has been extensively discouraged in the literature due to the very high likelihood of contamination of the laboratory, what will ultimately increase the false positive samples due to this cross-contamination ( Amin Almasi, 2012 ; Chen et al, 2023 ; Hanyue et al, 2023 ; Karthik et al, 2014 ; N. B. Quoc et al, 2021 ; Nguyen Bao Quoc et al, 2018 ).…”
Section: Discussionmentioning
confidence: 99%