“…Keeping the dye out of the reaction was important because, at the concentration needed to generate a naked-eye color change, it would have resulted in reaction inhibition ( Oscorbin et al, 2016 ; Quyen et al, 2019 ). It was confirmed that the dye could be added directly to the reaction after the amplification; however, it was discarded as the tubes will have to be opened, and it has been extensively discouraged in the literature due to the very high likelihood of contamination of the laboratory, what will ultimately increase the false positive samples due to this cross-contamination ( Amin Almasi, 2012 ; Chen et al, 2023 ; Hanyue et al, 2023 ; Karthik et al, 2014 ; N. B. Quoc et al, 2021 ; Nguyen Bao Quoc et al, 2018 ).…”