Closely related phytoplankton species produce similar suites of dissolved organic matter

Becker, Jamie W.; Berube, Paul M.; Follett, Christopher L.; Waterbury, John; Chisholm, Sallie W.; DeLong, Edward F.; Repeta, Daniel J.

doi:10.3389/fmicb.2014.00111

Cited by 133 publications

(122 citation statements)

References 62 publications

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“…One-carbon compounds such as methanol (27,29,31), as well as several sulfonates (32), have recently been added to the list. Chemical analyses concur: Photosynthate released directly from phytoplankton is highly complex, consisting of hundreds of different compounds (33,34). This "dissolved primary production"-the material released from living phytoplankton-supports a major fraction of labile carbon flux in the surface ocean (35).…”

Section: Which Compounds Represent the Largest Conduits Of Carbon Flumentioning

confidence: 92%

See 1 more Smart Citation

Deciphering ocean carbon in a changing world

Moran

Kujawinski

Stubbins

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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267

233

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Dissolved organic matter (DOM) in the oceans is one of the largest pools of reduced carbon on Earth, comparable in size to the atmospheric CO 2 reservoir. A vast number of compounds are present in DOM, and they play important roles in all major element cycles, contribute to the storage of atmospheric CO 2 in the ocean, support marine ecosystems, and facilitate interactions between organisms. At the heart of the DOM cycle lie molecular-level relationships between the individual compounds in DOM and the members of the ocean microbiome that produce and consume them. In the past, these connections have eluded clear definition because of the sheer numerical complexity of both DOM molecules and microorganisms. Emerging tools in analytical chemistry, microbiology, and informatics are breaking down the barriers to a fuller appreciation of these connections. Here we highlight questions being addressed using recent methodological and technological developments in those fields and consider how these advances are transforming our understanding of some of the most important reactions of the marine carbon cycle.dissolved organic matter | marine microbes | cyberinfrastructure

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Section: Which Compounds Represent the Largest Conduits Of Carbon Flumentioning

confidence: 92%

“…Correspondingly, nontargeted workflows are helping us to discover new molecules we didn't know to look for (33,39,70,105,135). Newly developed informatics approaches are supporting data mining across multiple studies and systems (136,137) (see Box 1).…”

Section: The Next Step: Prototypical Molecules Of the Marine Carbon Cmentioning

confidence: 99%

Deciphering ocean carbon in a changing world

Moran

Kujawinski

Stubbins

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

267

233

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“…3f, 4d, 6a, b; 9; Table 2), thereby suggesting CDOM was produced by heterotrophic bacteria from their assimilation of labile organic compounds released by Synechococcus spp. Indeed, the latter has been shown to release DOM (Bronk, 1999;Becker et al, 2014) that may be directly used to support heterotrophic activity (Nagata, 2000;Lefort and Gasol, 2014). These (non-coloured) labile organic substrates issued from Synechococcus spp.…”

Section: Particulate Absorptionmentioning

confidence: 99%

Evolution of dissolved and particulate chromophoric materials during the VAHINE mesocosm experiment in the New Caledonian coral lagoon (south-west Pacific)

et al. 2016

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Abstract. In the framework of the VAHINE project, we investigated the spectral characteristics and the variability of dissolved and particulate chromophoric materials throughout a 23-day mesocosm experiment conducted in the south-west Pacific at the mouth of the New Caledonian coral lagoon (22 • 29.073 S-166 • 26.905 E) from 13 January to 4 February 2013. Samples were collected in a mesocosm fertilized with phosphate at depths of 1, 6 and 12 m and in the surrounding waters. Light absorption coefficients of chromophoric dissolved organic matter (CDOM) [a g (λ)] and particulate matter [a p (λ)] were determined using a point-source integrating-cavity absorption meter (PSICAM), while fluorescent DOM (FDOM) components were determined from excitation-emission matrices (EEMs) combined with parallel factor analysis (PARAFAC). The evolutions of a g (λ) and a p (λ) in the mesocosm were similar to those of total chlorophyll a concentration, Synechococcus spp. and picoeukaryote abundances, bacterial production, particulate organic nitrogen and total organic carbon concentrations, with roughly a decrease from the beginning of the experiment to days 9-10, and an increase from days 9-10 to the end of the experiment. In the surrounding waters, the same trend was observed but the increase was much less pronounced, emphasizing the effect of the phosphate fertilization on the mesocosm's plankton community. Correlations suggested that both Synechococcus cyanobacteria and heterotrophic bacteria were strongly involved in the production of CDOM and absorption of particulate matter. The increase in phytoplankton biomass during the second part of the experiment led to a higher contribution of particulate material in the absorption budget at 442 nm. The three FDOM components identified (tryptophan-, tyrosine-and ultraviolet C (UVC) humic-like fluorophores) did not follow the evolution of CDOM and particulate matter, suggesting they were driven by different production/degradation processes. Finally, the results of this work support the idea there is indirect coupling between the dynamics of N 2 fixation and that of chromophoric material via the stimulation of Synechococcus bloom.

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“…The physiological mechanisms underlying the different types of interrelationships that occur among Prochlorococcus and other microbes undoubtedly extend far beyond reactive oxygen species scavenging and fixed carbon exchange (Becker et al, 2014). As a step toward further understanding the underpinnings of these interactions, we examined the transcriptional responses of Prochlorococcus strain NATL2A to growth in pure culture vs in co-culture with the marine heterotroph, Alteromonas macleodii strain MIT1002 (Biller et al, 2015b) (hereafter 'Alteromonas').…”

Section: Introductionmentioning

confidence: 99%

Torn apart and reunited: impact of a heterotroph on the transcriptome of Prochlorococcus

2016

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Microbial interactions, whether direct or indirect, profoundly affect the physiology of individual cells and ultimately have the potential to shape the biogeochemistry of the Earth. For example, the growth of Prochlorococcus, the numerically dominant cyanobacterium in the oceans, can be improved by the activity of co-occurring heterotrophs. This effect has been largely attributed to the role of heterotrophs in detoxifying reactive oxygen species that Prochlorococcus, which lacks catalase, cannot. Here, we explore this phenomenon further by examining how the entire transcriptome of Prochlorococcus NATL2A changes in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002, with which it was co-cultured for years, separated and then reunited. Significant changes in the Prochlorococcus transcriptome were evident within 6 h of initiating co-culture, with groups of transcripts changing in different temporal waves. Many transcriptional changes persisted throughout the 48 h experiment, suggesting that the presence of the heterotroph affected a stable shift in Prochlorococcus physiology. These initial transcriptome changes largely corresponded to reduced stress conditions for Prochlorococcus, as inferred from the depletion of transcripts encoding DNA repair enzymes and many members of the 'high light inducible' family of stress-response proteins. Later, notable changes were seen in transcripts encoding components of the photosynthetic apparatus (particularly, an increase in PSI subunits and chlorophyll synthesis enzymes), ribosomal proteins and biosynthetic enzymes, suggesting that the introduction of the heterotroph may have induced increased production of reduced carbon compounds for export. Changes in secretion-related proteins and transporters also highlight the potential for metabolic exchange between the two strains.

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Closely related phytoplankton species produce similar suites of dissolved organic matter

Cited by 133 publications

References 62 publications

Deciphering ocean carbon in a changing world

Deciphering ocean carbon in a changing world

Evolution of dissolved and particulate chromophoric materials during the VAHINE mesocosm experiment in the New Caledonian coral lagoon (south-west Pacific)

Torn apart and reunited: impact of a heterotroph on the transcriptome of Prochlorococcus

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