Closely related Salmonella Derby strains triggered distinct gut microbiota alteration

Abstract

Background Salmonella Derby is one of the most predominant Salmonella serotypes that seriously threatens food safety. This bacterium can be further differentiated to sub-populations with different population sizes; however, whether and how the S. Derby–gut microbiota interactions affect epidemic patterns of S. Derby sub-populations remain largely unknown. Results We selected two representative strains, 14T and 14C, which represent rarely distributed and prevalent sub-populations of the S. Derby ST40 group, respectively, to address this question using a mouse model. Effects of oral administration of both strains was monitored for 14 days. Alpha diversity of gut microbiota at early stages of infection (4 h post infection) was higher in 14C-treated mice and lower in 14T-treated mice compared with controls. Strain 14T triggered stronger inflammation responses but with lower pathogen titer in spleen compared with strain 14C at 14 days post infection. Certain known probiotic bacteria that can hinder colonization of Salmonella, such as Bifidobacteriaceae and Akkermansiaceae, exhibited increased relative abundance in 14T-treated mice compared with 14C-treated mice. Our results also demonstrated that Ligilactobacillus strains isolated from gut microbiota showed stronger antagonistic activity against strain 14T compared with strain 14C. Conclusions We identified how S. Derby infection affected gut microbiota composition, and found that the 14T strain, which represented a rarely distributed S. Derby sub-population, triggered stronger host inflammation responses and gut microbiota disturbance compared with the 14C strain, which represented a prevalent S. Derby sub-population. This study provides novel insights on the impacts of gut microbiota on the epidemic patterns of Salmonella populations.

SaaS sRNA promotes Salmonella intestinal invasion via modulating MAPK inflammatory pathway

“…Also, the depletion of Parabacteroides and Lachnospiraceae_uncultured was confirmed by multiple Salmonella infection models, especially those involving higher virulence Salmonella infection. 56 , 60 While for Ligilactobacillus , a probiotic bacteria used to treat enteric infections, 61 the increased abundance could be utilized by Salmonella to suppress inflammatory response for immune escape as reported here, 62 confirming the negative correlation between it and pro-inflammatory factors. On the other hand, SaaS increased the relative abundance of deleterious bacteria or making general bacteria harmful such as the Mycobacterium .…”

Assessment of beneficial effects and identification of host adaptation-associated genes of Ligilactobacillus salivarius isolated from badgers

“…The anti- Salmonella effects of L. salivarius strains were determined using the agar spot test, as described by Schillinger [ 63 ], with a pathogenic strain S. Derby 14T originating from swine serving as an indicator [ 64 ]. After incubation in MRS liquid medium at 37°C for 24 h, 10 µL of each L. salivarius culture was inoculated in the center of MRS agar plates, air dried, and incubated at 37°C for an additional 24 h. Following this, 10 µL of overnight grown S. Derby 14T culture was added to Luria–Bertani (LB) semi-solid medium and mixed well.…”

Effects of Salmonella Typhimurium Infection on the Gut Microbiota of Cherry Valley Meat Ducks

“…Gut microbiota can control harmful microbial invasion through habitat filters, including diet, host-derived resources, and microbiota-derived metabolites such as short-chain fatty acids [ 17 ]. Many studies have shown that butyric acid, one of the short-chain fatty acids, has the effect of inhibiting Salmonella [ 22 , 23 , 24 ]. Ruminococcaceae [ 25 , 26 ] and Lachnospiraceae [ 27 ] are important producers of butyric acid in the intestine.…”