Biochar, a carbon (C)-rich material obtained from the
thermochemical
conversion of biomass under oxygen-limited environments, has been
proposed as one of the most promising materials for C sequestration
and climate mitigation in soil. The C sequestration contribution of
biochar hinges not only on its fused aromatic structure but also on
its abiotic and biotic reactions with soil components across its entire
life cycle in the environment. For instance, minerals and microorganisms
can deeply participate in the mineralization or complexation of the
labile (soluble and easily decomposable) and even recalcitrant fractions
of biochar, thereby profoundly affecting C cycling and sequestration
in soil. Here we identify five key issues closely related to the application
of biochar for C sequestration in soil and review its outstanding
advances. Specifically, the terms use of biochar, pyrochar, and hydrochar,
the stability of biochar in soil, the effect of biochar on the flux
and speciation changes of C in soil, the emission of nitrogen-containing
greenhouse gases induced by biochar production and soil application,
and the application barriers of biochar in soil are expounded. By
elaborating on these critical issues, we discuss the challenges and
knowledge gaps that hinder our understanding and application of biochar
for C sequestration in soil and provide outlooks for future research
directions. We suggest that combining the mechanistic understanding
of biochar-to-soil interactions and long-term field studies, while
considering the influence of multiple factors and processes, is essential
to bridge these knowledge gaps. Further, the standards for biochar
production and soil application should be widely implemented, and
the threshold values of biochar application in soil should be urgently
developed. Also needed are comprehensive and prospective life cycle
assessments that are not restricted to soil C sequestration and account
for the contributions of contamination remediation, soil quality improvement,
and vegetation C sequestration to accurately reflect the total benefits
of biochar on C sequestration in soil.