Closing-upon-repair DNA tetrahedron nanoswitch for FRET imaging the repair activity of 8-oxoguanine DNA glycosylase in living cells

Repair-driven DNA tetrahedral nanomachine combined with DNAzyme for 8-oxo guanine DNA glycosylase activity assay, drug screening and intracellular imaging

“…Tetrahedral DNA nanostructures (TDN), as one of the most representative 3D DNA nanostructures, have emerged as promising nanomaterials for enzyme intracellular bioimaging due to their exceptional biocompatibility, controllable structure, and endonuclease resistance. [16][17][18][19][20] In the meantime, the introduction of DNA amplification strategies has substantially enhanced the performance of DNA-based biosensors by providing a sensitive signal. 21,22 As a class of DNA molecules with high catalytic activity, DNAzymes have been widely recognized as a potent signal amplification tool for the development of versatile biosensors.…”