Clostridium Butyricum CGMCC0313.1 Modulates Lipid Profile, Insulin Resistance and Colon Homeostasis in Obese Mice

Abstract

Obesity is associated with a cluster of metabolic disorders and systemic low-grade inflammation involving multiple organs. Recent findings have suggested that intestine is a key organ altered in response to high fat diet (HFD) feeding. Probiotics mainly lactobacillus strains have earlier been implicated in alleviating metabolic disorders. Here we aimed to examine the effects of a naturally occurring butyrate-producing probiotic clostridium butyricum CGMCC0313.1 (CB0313.1) in limiting the development of HFD-induced obesity. Mice treated with CB0313.1 exhibited reduced lipid accumulation in liver and serum, lower circulating insulin levels and improved glucose tolerance and insulin sensitivity. Furthermore, CB0313.1 administration reversed the HFD-induced colonic inflammation as evidenced by reduced tumor necrosis factor (TNF)-α level and increases the interleukin (IL)-10 and IL-22 levels in colon tissue. Additionally to colonic inflammation, CB0313.1 also reduced the colon permeability by upregulating the tight junction (TJ) proteins (claudin-1 and occludin) and contributed to a decreased circulating endotoxin level. In colon content, CB0313.1 administration restored the reduced production of butyrate and other short chain fatty acids (SCFAs) caused by HFD feeding. In adipose tissue, lower transcriptional levels of pro-inflammatory TNF-α, IL-6, IL-1β and monocyte chemotactic protein (MCP)-1 in adipose tissue were observed in CB0313.1-treated mice. Collectively, our data demonstrated that CB0313.1, targeting colon inflammation and permeability, ameliorated HFD-induced obesity, insulin resistance as well as adipose inflammation.

Clostridium butyricum Strains Suppress Experimental Acute Pancreatitis by Maintaining Intestinal Homeostasis

“…It has been demonstrated that CBM588 alleviated acute experimental colitis and high‐fat diet‐induced non‐alcoholic fatty liver disease . We have earlier shown that CB0313.1 improves type 1 diabetes and obesity . Herein, we extended beneficial effects of C. butyricum to experimental AP.…”

Specific inulin‐type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrier function, and microbiota homeostasis

“…Samples were then centrifuged at 14 000 g for 15 min at 4°C and supernatants were used for analysis. We used parameters of GC‐MS as previously described . Next‐generation sequencing was performed on an Illumina MiSeq system.…”

Possible Prophylaxes of Aloe Vera Gel Ingestion to Butyrate Metabolism

“…In adipose tissue, lower transcriptional levels of pro-inflammatory TNF-α, IL-6, IL-1β and monocyte chemotactic protein-1 in adipose tissue were observed in CB0313.1-treated mice. Collectively, the authors demonstrated that CB0313.1, targeting colon inflammation and permeability, ameliorated HFD-induced obesity, insulin resistance as well as adipose inflammation [7] . In the review: possible efficacy of Aloe Vera gel metabolites in long-term ingestion to insulin sensitivity, Yagi A. reported the beneficial effects of manno-oligosaccharide in Aloe Vera gel to gutbrain (hypothalamus) axis of hepatic glucose production and insulin sensitivity, and proposed an assumption based on a brain-liverintestine circuit to coordinate with insulin sensitivity of endogenous glucose production [1] .…”

“…Mice treated with CB0313.1 exhibited reduced lipid accumulation in liver and serum, lower circulating insulin levels and improved glucose tolerance and insulin sensitivity. CB0313.1, targeting colon inflammation and permeability, ameliorated HFD-induced obesity, insulin resistance as well as adipose inflammation [7] . Present review summarizes various mechanisms in which butyrate may influence brain health in a number of different neurological disorders.…”

“…Measurement of expired 13 CO2 after rectally administering [1][2][3][4][5][6][7][8][9][10][11][12][13] C]-butyrate in active and quiescent UC appears to be a promising and reliable method for evaluating disease activity and metabolic changes associated with amelioration of inflammation [10] . Gao Z. and his group examined the role of butyrate in the regulation of insulin sensitivity in dietary-obese C57BL/6J mice.…”