Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7alpha-dehydroxylating activity.

Kitahara, Maki; Takamine, Fusae; Imamura, Teisuke; Benno, Yoshimi

doi:10.1099/00207713-51-1-39

Cited by 168 publications

(134 citation statements)

References 22 publications

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“…This species belongs to Clostridium cluster XI which also includes C. difficile. 39 The latter is known to increase in relative abundance during broad-spectrum AB therapy, sometimes leading to AB-associated diarrhea and pseudomembranous colitis. 10,40,41 In this study, C. hiranonis decreased in relative abundance due to AB treatment (mean www.tandfonline.comdecrease from 0.29% to 0.01% at the DNA level).…”

Section: Ab Therapy Leads To a Reduction Of Beneficial Otusmentioning

confidence: 99%

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Heinsen

Knecht

Neulinger³

et al. 2015

Gut Microbes

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(2015) Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin, Gut Microbes, 6:4, 243-254, DOI: 10.1080/19490976.2015 To link to this article: https://doi.org/10. 1080/19490976.2015 Keywords: 16S rRNA gene, antibiotics, lumen, microbiota, mucosaGut microbiota play a key role in the host's health system. Broad antibiotic therapy is known to disrupt the microbial balance affecting pathogenic as well as host-associated microbes. The aim of the present study was to investigate the influence of antibiotic paromomycin on the luminal and mucosa-associated microbiota at the DNA (abundance) and RNA (potential activity) level as well as to identify possible differences. The influence of antibiotic treatment on intestinal microbiota was investigated in 5 healthy individuals (age range: 20-22 years). All participants received the antibiotic paromomycin for 3 d. Fecal samples as well as sigmoidal biopsies were collected before and immediately after cessation of antibiotic treatment as well as after a recovery phase of 42 d. Compartment-and treatment statusspecific indicator operational taxonomic units (OTUs) as well as abundance-and activity-specific patterns were identified by 16S rRNA and 16S rRNA gene amplicon libraries and high-throughput pyrosequencing. Microbial composition of lumen and mucosa were significantly different at the DNA compared to the RNA level. Antibiotic treatment resulted in changes of the microbiota, affecting the luminal and mucosal bacteria in a similar way. Several OTUs were identified as compartment-and/or treatment status-specific. Abundance and activity patterns of some indicator OTUs differed considerably. The study shows fundamental changes in composition of gut microbiota under antibiotic therapy at both the potential activity and the abundance level at different treatment status. It may help to understand the complex processes of gut microbiota changes involved in resilience mechanisms and on development of antibiotic-associated clinical diseases.

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Section: Ab Therapy Leads To a Reduction Of Beneficial Otusmentioning

confidence: 99%

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Heinsen

Knecht

Neulinger³

et al. 2015

Gut Microbes

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“…The phylogenetic position of the two strains among Bacteroides species and representative members of the genera Prevotella, Bacteroides, Porphyromonas and Tannerella are shown in Fig. 1 (Kitahara et al, 2001) after incubation for 12 h at 37 u C, and purified by using the methods of Saito & Miura (1963). Cells were disrupted with lysozyme (Wako Pure Chemical Industries) and SDS, extracted with phenol, and the DNA was recovered using alcohol precipitation and suspended in TE buffer (1 mM EDTA, 10 mM Tris/HCl, pH 8?0).…”

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confidence: 99%

“…T and 176 supported the affiliation of the novel strains with members of the genus Bacteroides, which have a G+C content of 40-48 mol% (Shah, 1992 T was extracted from cells harvested from EGF broth (Kitahara et al, 2001). DNA-DNA hybridization was performed by using the photobiotin-labelling method of Ezaki et al (1989), using a microplate reader (FluoroskanAscent; Labsystems).…”

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confidence: 99%

Bacteroides finegoldii sp. nov., isolated from human faeces

Bakir

Kitahara

Sakamoto

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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“…Extraction and purification of bacterial DNA was performed according to the methods of Kitahara et al (2001) and purified by the method of Saito & Miura (1963). Strains of L. fructivorans, L. homohiochii and L. sanfranciscensis were not used in this study because the sequence similarities between the new isolates and the type strains of these three species were lower than the recommended value for species differentiation as proposed by Stackebrandt & Ebers (2006).…”

mentioning

confidence: 99%

“…Levels of DNA-DNA relatedness between the new isolates and L. lindneri JCM 11027 T , and the DNA G+C contents of the isolates were determined according to methods described by Kitahara et al (2001). Extraction and purification of bacterial DNA was performed according to the methods of Kitahara et al (2001) and purified by the method of Saito & Miura (1963).…”

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confidence: 99%

Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

Endo

Futagawa-Endo

Sakamoto

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Three strains of fructophilic lactic acid bacteria were isolated from flowers in South Africa. The isolates formed a subcluster in the Lactobacillus buchneri phylogenetic group, closely related to Lactobacillus fructivorans, Lactobacillus homohiochii, Lactobacillus lindneri and Lactobacillus sanfranciscensis according to phylogenetic analysis based on the 16S rRNA gene sequences. Levels of DNA-DNA relatedness indicated that the three strains belonged to the same taxon and formed a genetically distinct group, well separated from their phylogenetic relatives. The three strains produced acids from only two of the 49 carbohydrates tested, i.e. D-glucose and Dfructose. D-Fructose was more rapidly fermented than D-glucose. Good growth was recorded on D-fructose or D-glucose in the presence of external electron acceptors. However, delayed growth was recorded on D-glucose without electron acceptors. The novel strains produced lactic acid, ethanol and acetic acid from D-glucose at a ratio of 1 : 0.8 : 0.2. These characteristics were distinct from other species of the genus Lactobacillus. Based on the data provided, the three isolates represent a fructophilic and novel species of the genus Lactobacillus, for which the name Lactobacillus florum sp. nov. is proposed. The type strain is F9-1 T (5JCM 16035 T 5DSM 22689 T 5NRIC 0771 T ).Fructophilic lactic acid bacteria (LAB) are a group of LAB described recently (Endo & Okada, 2008). These organisms are known to have unique characteristics, i.e. they grow well on D-fructose or D-glucose in the presence of external electron acceptors, but grow poorly on D-glucose without electron acceptors (Endo & Okada, 2008). Pyruvate and oxygen are used as electron acceptors. At the time of writing, only five species with these characteristics have been described, Fructobacillus fructosus, Fructobacillus durionis, Fructobacillus ficulneus and Fructobacillus pseudoficulneus (Endo & Okada, 2008) and Lactobacillus kunkeei (Endo et al., 2009).During a study of fructophilic LAB, three strains were isolated from flowers in South Africa (Endo et al., 2009). The isolates shared several common characteristics with known fructophilic LAB, but also had a few different characteristics. They formed a subcluster within the Lactobacillus buchneri cluster on phylogenetic analysis based on their 16S rRNA gene sequences and were closely related to Lactobacillus lindneri. Levels of DNA2DNA similarity and biochemical characteristics showed clear separation of the isolates from L. lindneri. This paper describes the taxonomic study of the three novel isolates and their classification as a novel species of the genus Lactobacillus. The three novel strains, F9-1 T , F9-2 and F17, grew well in FYP broth, but poorly in GYP broth and were thus regarded as fructophilic LAB. They were preserved at 280 u C in nutrient broth containing 20 % (v/v) glycerol.The 16S rRNA gene sequences of the three isolates were determined according to a previously described method (Endo & Okada, 2005). The closest recognized relatives of t...

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Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7alpha-dehydroxylating activity.

Cited by 168 publications

References 22 publications

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Bacteroides finegoldii sp. nov., isolated from human faeces

Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

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