Clostridium ljungdahlii as a biocatalyst in microbial electrosynthesis – Effect of culture conditions on product formation

Im, Chaeho; Valgepea, Kaspar; Modin, Oskar; Nygård, Yvonne

doi:10.1016/j.biteb.2022.101156

Cited by 14 publications

(10 citation statements)

References 53 publications

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“…When connected to the 1000 Ω and in the presence of HCO 3 − , the cathode potentials achieved −400 ± 10 mV and −422 ± 10 mV after, respectively, two and six hours, while pH values in catholyte increased to 7.1 ± 0.2. This result is consistent with previous reports indicating neutral pH values in the presence of small amounts of ethanol besides acetate when using acetogenic bacteria in MECs for CO 2 capture [ 22 , 24 ]. Even though we did not investigate the presence of ethanol in catholyte, its biosynthesis cannot be excluded.…”

Section: Resultssupporting

confidence: 93%

“…After 48 h of incubation at 30 °C in anaerobiosis, 100 μL of microbial suspension was inoculated in 9.9 mL of a further modified TSYEM, where tryptone was replaced with NaHCO 3 (0.5% w / v ), final pH 6.8 ± 0.2 The catholyte for CO 2 capture from a gaseous mix was composed of yeast extract (YE) 2.5 g/L; (NH 4 ) 2 SO 4 0.5 g/L; FeSO 4 0.025 g/L, with 6.8 ± 0.2 as final pH. YE was added as a necessary component for clostridia growth and metabolic functions, supplying micronutrients and growth factors [ 22 , 24 , 28 ]. The bioanode strains, Shewanella oneidensis MR1 (ATCC 700550) and Pseudomonas aeruginosa PA1430/CO1 (DSM 19882), were cultured in nutrient broth (NA, Oxoid, Basingstoke, UK) according to the supplier’s instructions and incubated at 30 °C for 24 h. Both strains were subcultured in tryptic soy agar (TSA, Oxoid, Basingstoke, UK) plates, pH 7.2 ± 0.2, and maintained for use in later experiments.…”

Section: Methodsmentioning

confidence: 99%

“…For example, organic substrates in the catholyte might reduce or even prevent CO 2 assimilation, fostering the establishment of a heterothrophic instead of an autothrophic or mixothrophic metabolism. Therefore, the set-up of MET devices requires adequate preliminary studies [ 17 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

et al. 2023

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The need for greener processes to satisfy the demand of platform chemicals together with the possibility of reusing CO2 from human activities has recently encouraged research on the set-up, optimization, and development of bioelectrochemical systems (BESs) for the electrosynthesis of organic compounds from inorganic carbon (CO2, HCO3−). In the present study, we tested the ability of Clostridium saccharoperbutylacetonicum N1-4 (DSMZ 14923) to produce acetate and D-3-hydroxybutyrate from inorganic carbon present in a CO2:N2 gas mix. At the same time, we tested the ability of a Shewanella oneidensis MR1 and Pseudomonas aeruginosa PA1430/CO1 consortium to provide reducing power to sustain carbon assimilation at the cathode. We tested the performance of three different systems with the same layouts, inocula, and media, but with the application of 1.5 V external voltage, of a 1000 Ω external load, and without any connection between the electrodes or external devices (open circuit voltage, OCV). We compared both CO2 assimilation rate and production of metabolites (formate, acetate 3-D-hydroxybutyrate) in our BESs with the values obtained in non-electrogenic control cultures and estimated the energy used by our BESs to assimilate 1 mol of CO2. Our results showed that C. saccharoperbutylacetonicum NT-1 achieved the maximum CO2 assimilation (95.5%) when the microbial fuel cells (MFCs) were connected to the 1000 Ω external resistor, with the Shewanella/Pseudomonas consortium as the only source of electrons. Furthermore, we detected a shift in the metabolism of C. saccharoperbutylacetonicum NT-1 because of its prolonged activity in BESs. Our results open new perspectives for the utilization of BESs in carbon capture and electrosynthesis of platform chemicals.

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Section: Resultssupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

et al. 2023

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“…Acetate is the most abundant product of gas fermentation, and therefore, has an essential role in the establishment of the co-culture. Lactate is a minor fermentation product of acetogens grown on syngas or CO 2 /H 2 (Valgepea et al, 2018 ; Im et al, 2022 ), but a major fermentation product of acetogens grown on sugars (Drake and Daniel, 2004 ). Acetogens could be engineered toward autotrophic lactate production from CO 2 /H 2 (Mook et al, 2022 ) or syngas, thereby facilitating butyrate production in co-cultivation with C. beijerinckii without the need of adding an additional carbon source.…”

Section: Discussionmentioning

confidence: 99%

Model-driven approach for the production of butyrate from CO2/H2 by a novel co-culture of C. autoethanogenum and C. beijerinckii

et al. 2022

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One-carbon (C1) compounds are promising feedstocks for the sustainable production of commodity chemicals. CO2 is a particularly advantageous C1-feedstock since it is an unwanted industrial off-gas that can be converted into valuable products while reducing its atmospheric levels. Acetogens are microorganisms that can grow on CO2/H2 gas mixtures and syngas converting these substrates into ethanol and acetate. Co-cultivation of acetogens with other microbial species that can further process such products, can expand the variety of products to, for example, medium chain fatty acids (MCFA) and longer chain alcohols. Solventogens are microorganisms known to produce MCFA and alcohols via the acetone-butanol-ethanol (ABE) fermentation in which acetate is a key metabolite. Thus, co-cultivation of an acetogen and a solventogen in a consortium provides a potential platform to produce valuable chemicals from CO2. In this study, metabolic modeling was implemented to design a new co-culture of an acetogen and a solventogen to produce butyrate from CO2/H2 mixtures. The model-driven approach suggested the ability of the studied solventogenic species to grow on lactate/glycerol with acetate as co-substrate. This ability was confirmed experimentally by cultivation of Clostridium beijerinckii on these substrates in batch serum bottles and subsequently in pH-controlled bioreactors. Community modeling also suggested that a novel microbial consortium consisting of the acetogen Clostridium autoethanogenum, and the solventogen C. beijerinckii would be feasible and stable. On the basis of this prediction, a co-culture was experimentally established. C. autoethanogenum grew on CO2/H2 producing acetate and traces of ethanol. Acetate was in turn, consumed by C. beijerinckii together with lactate, producing butyrate. These results show that community modeling of metabolism is a valuable tool to guide the design of microbial consortia for the tailored production of chemicals from renewable resources.

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“…Acetate is the most abundant product in gas fermentation and therefore, it is key in the establishment of the co-culture. Lactate has been reported to be a minor fermentation product by acetogens grown on syngas or CO 2 /H 2 (9, 48), but a major fermentation product by acetogens grown on sugars (49). Therefore, acetogens can be engineered to increase lactate production on CO 2 /H 2 (50), or engineered to grow on CO 2 , instead of sugars, producing lactate, and thus, produce butyrate in co-cultivation with C. beijerinckii .…”

Section: Discussionmentioning

confidence: 99%

Model-driven approach for the production of butyrate from CO₂/H₂by a novel co-culture ofC. autoethanogenumandC. beijerinckii

Benito-Vaquerizo

Nouse

Schaap

et al. 2022

Preprint

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One-carbon (C1) compounds are promising feedstocks for sustainable production of commodity chemicals. CO2 is a particularly advantageous C1-feedstock since it is an unwanted industrial off-gas that can be converted to valuable products while reducing its atmospheric levels. Acetogens are known microorganisms that can grow on CO2/H2 and syngas converting these substrates into ethanol and acetate. Co-cultivation of acetogens with microbes that can further process such products can expand the variety of products to, for example, medium chain fatty acids (MCFA) and longer chain alcohols. Solventogens are microorganisms known to produce MCFA and alcohols via the acetone, butanol and ethanol (ABE) fermentation in which acetate is a key metabolite. Thus, co-cultivation of a solventogen and acetogen in a consortium provides a potential platform to produce valuable chemicals from CO2. In this study, metabolic modelling was implemented to design a new co-culture of an acetogen and a solventogen to produce butyrate from CO2/H2 mixtures. The model-driven approach suggested the ability of the studied solventogenic species to grow on lactate/glycerol with acetate as co-substrate. This ability was confirmed experimentally by cultivation of Clostridium beijerinckii on these substrates in serum bottles and subsequently in pH-controlled bioreactors. Community modelling also suggested that a novel microbial consortium consisting of the acetogen Clostridium autoethanogenum, and the solventogen C. beijerinckii would be feasible and stable. On the basis of this prediction, a co-culture was experimentally established. C. autoethanogenum grew on CO2/H2 producing acetate and traces of ethanol. Acetate was in turn, consumed by C. beijerinckii together with lactate, producing butyrate. These results show that community modelling of metabolism is a valuable tool to guide the design of microbial consortia for the tailored production of chemicals from renewable resources.

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Clostridium ljungdahlii as a biocatalyst in microbial electrosynthesis – Effect of culture conditions on product formation

Cited by 14 publications

References 53 publications

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

Model-driven approach for the production of butyrate from CO2/H2 by a novel co-culture of C. autoethanogenum and C. beijerinckii

Model-driven approach for the production of butyrate from CO₂/H₂by a novel co-culture ofC. autoethanogenumandC. beijerinckii

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Clostridium ljungdahlii as a biocatalyst in microbial electrosynthesis – Effect of culture conditions on product formation

Cited by 14 publications

References 53 publications

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4

Model-driven approach for the production of butyrate from CO2/H2 by a novel co-culture of C. autoethanogenum and C. beijerinckii

Model-driven approach for the production of butyrate from CO2/H2by a novel co-culture ofC. autoethanogenumandC. beijerinckii

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Model-driven approach for the production of butyrate from CO₂/H₂by a novel co-culture ofC. autoethanogenumandC. beijerinckii