2004
DOI: 10.1016/j.anaerobe.2004.04.002
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Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse

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Cited by 52 publications
(29 citation statements)
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“…By large, most of these studies have dealt with thermophilic bacteria. Only few studies concentrate on mesophilic conditions (Belyakova et al, 2006;Caccavo et al, 1994;Mogensen et al, 2005;Nakagawa et al, 2005;Thabet et al, 2004). Even less literature can be found on reactors for the biological elemental sulfur reduction and little is known about kinetic limitations; reactor parameters or coefficients; the ability of sulfate-reducing bacteria to form biofilms on sulfur surface in turbulent three-phase system; and mass and energy transport phenomena.…”
Section: Introductionmentioning
confidence: 97%
“…By large, most of these studies have dealt with thermophilic bacteria. Only few studies concentrate on mesophilic conditions (Belyakova et al, 2006;Caccavo et al, 1994;Mogensen et al, 2005;Nakagawa et al, 2005;Thabet et al, 2004). Even less literature can be found on reactors for the biological elemental sulfur reduction and little is known about kinetic limitations; reactor parameters or coefficients; the ability of sulfate-reducing bacteria to form biofilms on sulfur surface in turbulent three-phase system; and mass and energy transport phenomena.…”
Section: Introductionmentioning
confidence: 97%
“…Three media were prepared by subsequent addition to basal medium of the following electron sources: lactate (40 mM), acetate (20 mM), and H 2 /CO 2 (80/20, v/v), 2 bars or methanol (40 mM), acetate (20 mM), and H 2 /CO 2 (80/20, v/v), 2 bars as carbon and energy sources to enrich and isolate SRB and methanogens, respectively, but also fermentative bacteria possibly growing on yeast extract as energy source [27,30].…”
Section: Media For Enumeration and Culture Of Fermentative Bacteria Amentioning
confidence: 99%
“…The 16S rRNA gene was amplified by PCR using universal primers Fd1 and R6 [30] ( Table 2). The PCR mixture contained (20 l final volume): 2 l of 10× buffer (Sigma), 2 l of 25 mM MgCl 2 (Sigma), 0.2 l of 10 mM dNTP mixture, 0.2 l of a 50 pmol/l solution of each primer, 0.2 l of Taq polymerase (5 U/l; Sigma), qsp.…”
Section: Dna Extraction and Amplification Of 16s Rrna Gene Of Isolatementioning
confidence: 99%
“…Clostridium contains about 100 species that include ubiquitous common bacteria as well as important pathogens (5,14). Clostridium tunisiense is a proteolytic asaccharolytic sulfur-reducing bacterium which was first isolated from anaerobic mud of olive mill wastewaters with a high level of organic load pollution in Tunis (13). Many anaerobic bacteria were found and researched as digesters for waste treatment because of their saccharolytic ability (2,3,(8)(9)(10).…”
mentioning
confidence: 99%
“…lostridium tunisiense is a species of the Gram-positive, sporogenic, obligate anaerobes of genus Clostridium belonging to the Firmicutes (1,13). Clostridium contains about 100 species that include ubiquitous common bacteria as well as important pathogens (5,14).…”
mentioning
confidence: 99%