This study aims to determine the differentiation of the impact of stakeholders on the institutional configuration of the microeconomic ecosystem. The object of this study was the stakeholders of the microeconomic ecosystem. The study scope is the economic relations between the subjects of economic activity on the impact on the formation and institutional development of the microeconomic ecosystem. The analysis of previous studies is carried out, and the main stakeholders of microeconomic ecosystems are identified. These include public organizations, consumers, suppliers, competitors, educational and scientific organizations, government authorities, and social media. The method of PEST analysis of the factors of the company's microenvironment identifies social, technological, economic, political and cultural institutions of the company's external environment. Based on the analysis of the results of published scientific research, various levels of influence (strong, medium, weak) of the main stakeholders on the institutional configuration of the microeconomic ecosystem are determined. It is shown that the uneven importance of stakeholders in the formation of the institutional configuration of the microeconomic ecosystem can be the basis for the development of strategic plans for the development of ecosystems.