Clover Root Exudates Favor
            <i>Novosphingobium</i>
            sp. HR1a Establishment in the Rhizosphere and Promote Phenanthrene Rhizoremediation

Molina, Lázaro; Udaondo, Zulema; Montero-Curiel, María; Wittich, Rolf-Michael; García-Puente, Alicia; Segura, Ana

doi:10.1128/msphere.00412-21

Cited by 10 publications

(6 citation statements)

References 76 publications

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“…In our study, we have found that planting, addition of maltose and glycine can significantly facilitate the degradation of petroleum hydrocarbons in loamy soil and clayey soil. Molina et al (2021) found that the maltose concentration in the clover root exudates with bacterial presence was significantly lower than that of the exudates without bacteria. Maltose can enhance the degradation of total petroleum hydrocarbons, probably because it can provide a large amount of energy and nutrient source for rhizosphere microorganisms, increasing the density and of microorganisms (Al-Ali et al, 2018) and then promoting the removal of petroleum hydrocarbon in soil.…”

Section: Removal Effect Of Root Exudates On Total Petroleum Hydrocarb...mentioning

confidence: 87%

Impact of soil types and root exudates on cadmium and petroleum hydrocarbon phytoremediation by Sorghum sudanense, Festuca arundinace, and Lolium perenne

Chen

et al. 2022

Front. Ecol. Evol.

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With the development of industrialization, soils around the world have been polluted by heavy metals and oil to different degrees in recent years, and soil remediation has become a global problem. Phytoremediation has a wide application prospect because of its environmental friendliness and easy availability of materials.ObjectiveTo explore the effects of soil types and root exudates on the removal of cadmium and petroleum hydrocarbon in soils.MethodA pot experiments with three soil types (sandy, loamy and clayey) of the Changning-Weiyuan area of Sichuan province and three root exudates (citric acid, glycine, and maltose) were carried out using Sorghum sudanense (Piper) Stapf., Lolium perenne L., and Festuca arundinacea L. as test materials. Plants were grown in soils contaminated by cadmium and petroleum at different concentrations.ResultThe biomass of S. sudanense, the translocation ratio and removal rate of cadmium in S. sudanense decreased gradually with increasing soil cadmium concentration. The promotion effects of the three root exudates on S. sudanense were in the following order: citric acid > glycine > maltose. At the same cadmium pollution conditions, the biomass levels of S. sudanense in sandy, loamy, and clayey soils were in the following order: clayey soil > loamy soil > sandy soil. On the contrary, the concentration, translocation ratio, and removal rate of cadmium in S. sudanense grown in the different soils treated with root exudates were in the following order: sandy soil > loamy soil > clayey soil. Under the three soil conditions, the fresh weight of F. arundinacea (0.36 ~ 0.68 g) and S. sudanense (0.51 ~ 0.99 g) increased significantly (p < 0.05). The total petroleum hydrocarbons degradation efficiencies of F. arundinacea, L. perenne, and S. sudanense were high in sandy soil (34.27% ~ 60.52%). Changing the type of root exudate had a significant impact on the degradation of total petroleum hydrocarbons in sandy soil (p < 0.05).ConclusionThis study showed that soil types impacted the accumulation of cadmium and petroleum in plants. Phytoremediation of cadmium and petroleum contaminated soil could be enhanced by the application of root exudates. This study recommend that the method is suitable for field remediation of soils contaminated with mild cadmium and petroleum hydrocarbons.

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Section: Removal Effect Of Root Exudates On Total Petroleum Hydrocarb...mentioning

confidence: 87%

Impact of soil types and root exudates on cadmium and petroleum hydrocarbon phytoremediation by Sorghum sudanense, Festuca arundinace, and Lolium perenne

Chen

et al. 2022

Front. Ecol. Evol.

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“…Total RNA extraction and preparation of libraries were carried out as in Molina et al (2021) . Briefly, the RNA was extracted using the Trizol method (TRIzol RNA Isolation Reagents, Thermofisher Scientist), followed by DNAse treatment and purification with the RNeasy Mini Kit (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

“…Novosphingobium sp. HR1a is able to degrade low-, medium- and high-molecular weight polycyclic aromatic hydrocarbons ( Segura et al, 2017 ), and it has shown excellent potential for rhizoremediation ( Molina et al, 2021 ). Furthermore, this strain is able to grow in marine media ( Segura et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes

Segura

Molina

2023

Front. Microbiol.

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Although there is some evidence to suggest that LuxR-solo proteins participate in inter-species or even inter-kingdom communication, most of the LuxR-solo protein functions are unknown. We have characterized the LuxR402 regulator of Novosphingobium sp. HR1a, a bacterial strain with the ability to establish high numbers in the plant rhizosphere and able to degrade a wide range of polycyclic aromatic hydrocarbons. LuxR402 controls the aggregation state of the bacterial culture; cultures of a mutant strain lacking this regulator flocculate in less than 3 h without agitation. We have demonstrated that the bacterial surface of the mutant is highly hydrophobic and that the mutant cells assimilate sugars slower than the wild-type. The flocculation mechanism has been demonstrated to be involved in the survival of the strain under unfavorable conditions; the luxR402 gene is repressed and produces flocculation in the presence of salicylate, a substrate that, although being assimilated by Novosphingobium, is toxic to cells at high concentrations. The flocculation of cultures in industrial setups has mainly been achieved through the addition of chemicals; these studies open up the possibility of controlling the flocculation by regulating the level of expression of the luxR402 gene.

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“…Furthermore, these enzymes catalyse the oxidation of phenolic compounds and PAHs using hydrogen peroxide as the electron acceptor, transforming these molecules into more easily degradable compounds for the indigenous microbiota, and therefore, indirectly, detoxifying these environments. Similarly, root exudates of various plant species, such as fescue grass (Festuca arundinacea), switch grass (Panicum virgatum), maize (Zea mays L.), soybean, sorghum, alfalfa and clover, have the ability to enhance PAH biodegradation, probably because plant roots can stimulate soil microbial biomass and oxygen transport to the rhizosphere, thus facilitating the degradation process [113,114].…”

Section: Detoxification Of Pahs and Hms By Plantsmentioning

confidence: 99%

Biochemical and Metabolic Plant Responses toward Polycyclic Aromatic Hydrocarbons and Heavy Metals Present in Atmospheric Pollution

Molina

Segura

2021

Plants

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Heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) are toxic components of atmospheric particles. These pollutants induce a wide variety of responses in plants, leading to tolerance or toxicity. Their effects on plants depend on many different environmental conditions, not only the type and concentration of contaminant, temperature or soil pH, but also on the physiological or genetic status of the plant. The main detoxification process in plants is the accumulation of the contaminant in vacuoles or cell walls. PAHs are normally transformed by enzymatic plant machinery prior to conjugation and immobilization; heavy metals are frequently chelated by some molecules, with glutathione, phytochelatins and metallothioneins being the main players in heavy metal detoxification. Besides these detoxification mechanisms, the presence of contaminants leads to the production of the reactive oxygen species (ROS) and the dynamic of ROS production and detoxification renders different outcomes in different scenarios, from cellular death to the induction of stress resistances. ROS responses have been extensively studied; the complexity of the ROS response and the subsequent cascade of effects on phytohormones and metabolic changes, which depend on local concentrations in different organelles and on the lifetime of each ROS species, allow the plant to modulate its responses to different environmental clues. Basic knowledge of plant responses toward pollutants is key to improving phytoremediation technologies.

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Clover Root Exudates Favor Novosphingobium sp. HR1a Establishment in the Rhizosphere and Promote Phenanthrene Rhizoremediation

Cited by 10 publications

References 76 publications

Impact of soil types and root exudates on cadmium and petroleum hydrocarbon phytoremediation by Sorghum sudanense, Festuca arundinace, and Lolium perenne

Impact of soil types and root exudates on cadmium and petroleum hydrocarbon phytoremediation by Sorghum sudanense, Festuca arundinace, and Lolium perenne

LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes

Biochemical and Metabolic Plant Responses toward Polycyclic Aromatic Hydrocarbons and Heavy Metals Present in Atmospheric Pollution

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