CLP induces apoptosis in human leukemia K562 cells through Ca2+ regulating extracellular-related protein kinase ERK activation

Lipopeptide Biosurfactant Pseudofactin II Induced Apoptosis of Melanoma A 375 Cells by Specific Interaction with the Plasma Membrane

“…The mechanism of apoptosis induction may be based on the membrane permeabilisation [21] resulting from the interaction of PFII micelles with the plasma membrane, as evidenced by the release of LDH into the culture medium (Figure 7). Thus, the mode of action of PFII is different from pro-apoptotic activity of the previously described cyclic lipopeptide (CLP) [15] which did not affected membrane integrity.…”

“…Some of these molecules have been shown to induce apoptosis in tumor cells. For example, a lipopeptide produced by Bacillus subtilis has anti-tumor activity on LoVo cells [13] and a cyclic lipopeptide from Bacillus natto T-2 induces apoptosis in human leukemia K562 cells [14] by an increase in [Ca 2+ ] and Extracellular Signal-regulated Kinases (ERK) phosphorylation [15]. Glycolipids from Candida antarctica and their analogs have been implicated in growth arrest, apoptosis, and differentiation of mouse malignant melanoma and human HL60 cells [16], [17].…”

The Role of MAPK-ERK Pathway in 67-kDa Laminin Receptor-Induced FasL Expression in Human Cholangiocarcinoma Cells

“…PD98059 was used at concentrations that have been previously described [29,30]. Our results show that PD98059 (20 lM) significantly cut down the levels of phosphorylation of ERK and decreased the expression of FasL (Fig.…”

Eicosapentaenoic acid (EPA) induced apoptosis in HepG2 cells through ROS–Ca2+–JNK mitochondrial pathways

“…With Fluo-3 acetoxymethyl ester (Fluo-3/AM) as a fluorescent Ca 2+ indicator, cytoplasmic [Ca 2+ ] c change was measured by LSCM according to a previously described method [13]. HepG2 cells were treated with 75 lM EPA, and then incubated with Fluo-3/AM for 1 h at 37°C in the dark, washed with PBS (pH 7.2) thrice.…”