ClpP mediates antagonistic interaction of Lysobacter enzymogenes with a crop fungal pathogen

Yang, Mingming; Ren, Shuangshuang; Shen, Danyu; Chou, Shan‐Ho; Qian, Guoliang

doi:10.1016/j.biocontrol.2019.104125

Cited by 7 publications

(6 citation statements)

References 43 publications

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“…After 3‐day incubation at 28°C, the inhibition zones were photographed using the Nikon camera (D7100, Japan) in both antifungal and antibacterial assays. The area of the inhibition zone was calculated according to the following formula – area = π × (radius) 2 , in which the radius is the average radius of the longest axis and the shortest axis of the inhibition zones as described previously (Yang et al ., 2020). All the experiments were carried out three times and three replicates were used for each treatment.…”

Section: Methodsmentioning

confidence: 99%

Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system

Shen

Wang

Yang

et al. 2021

Environmental Microbiology

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Summary Soil microbiome comprises numerous microbial species that continuously interact with each other. Among the modes of diverse interactions, cell–cell killing may play a key role in shaping the microbiome composition. Bacteria deploy various secretion systems to fend off other microorganisms and Type IV Secretion System (T4SS) in pathogenic bacteria was shown to function as a contact‐dependent, inter‐bacterial killing system only recently. The present study investigated the role played by T4SS in the killing behaviour of the soilborne biocontrol bacterium Lysobacter enzymogenes OH11. Results showed that L. enzymogenes OH11 genome encompasses genes encoding all the components of T4SS and effectors potentially involved in inter‐bacterial killing system. Generation of knock‐out mutants revealed that L. enzymogenes OH11 uses T4SS as the main contact‐dependent weapon against other soilborne bacteria. The T4SS‐mediated killing behaviour of L. enzymogenes OH11 decreased the antibacterial and antifungal activity of two Pseudomonas spp. but at the same time, protected carrot from infection by Pectobacterium carotovorum. Overall, this study showed for the first time the involvement of T4SS in the killing behaviour of L. enzymogenes and its impact on the multiple interactions occurring in the soil microbiome.

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Section: Methodsmentioning

confidence: 99%

Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system

Shen

Wang

Yang

et al. 2021

Environmental Microbiology

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“…Dash-lined circles indicate the sites of HSAF spotting. Lengths of the longest axis and shortest axis of the inhibition zones were determined and averaged as the radius, R, according to an earlier report [ 33 ]. The inhibition zones were calculated by using the formula π × R 2 .…”

Section: Resultsmentioning

confidence: 99%

“…Antimicrobial activity assay was carried out according to our previous reports [ 33 , 34 ] with slight modifications. Briefly, a mycelium plug of the filamentous pathogen, Phytophthora capsici that was transferred from PDA (Potato Dextrose Agar) medium was inoculated on the center of 1/10 TSB agar plates.…”

Section: Methodsmentioning

confidence: 99%

“…After two-day incubate 28˚C, the antagonistic activity was monitored based on the inhibition zones around the colonies. For this, lengths of the longest axis and shortest axis of the inhibition zones were determined and averaged as the radius, according to an earlier report [33]. The inhibition zones were calculated by using the formula: area = π × (radius) 2 .…”

Section: Antimicrobial Activity Assaysmentioning

confidence: 99%

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An intrinsic mechanism for coordinated production of the contact-dependent and contact-independent weapon systems in a soil bacterium

et al. 2020

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Soil bacteria possess multiple weapons to fend off microbial competitors. Currently, we poorly understand the factors guiding bacterial decisions about weapon systems deployment. In this study, we investigated how such decisions are made by the soil bacterium Lysobacter enzymogenes, used in antifungal plant protection. We found that weapons production is guided by environmental cues. In rich media, which likely mimic environments crowded with other microbes, L. enzymogenes produces a contact-dependent weapon, type six secretion system (T6SS). In nutrient-poor media, likely dominated by filamentous oomycetes and fungi, L. enzymogenes synthesizes and secretes a heat-stable antifungal factor (HSAF), a contact-independent weapon. Surprisingly, the T6SS inner tube protein Hcp is accumulated intracellularly even in nutrient-poor media, when the T6SS is not assembled. We found that Hcp interacts with the transcription factor Clp required for activating HSAF biosynthesis operon expression. Hcp protects Clp from binding to c-di-GMP, an intracellular second messenger inhibiting DNA binding. The increased concentration of c-di-GMP-free Clp thus leads to higher gene expression and HSAF production. Therefore, when the contact-dependent weapon, T6SS, is not in use, accumulation of one of its structural components, Hcp, serves as a signal to enhance production of the contact-independent weapon, HSAF. The uncovered environment-dependent and auto-regulatory mechanisms shed light on the processes governing deployment of various weapon systems in environmental bacteria.

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“…Plate antifungal assay was performed as described previously (Yang, Ren, Shen, Chou, et al, 2020). In brief, 4 μl of crude extract of HSAF from the wild-type OH11 and its derivatives was spotted at the edge of 1/10 TSB agar plates inoculated at the plate centre with V. pyri strain SXYL134 (Table S1), which was transferred from potato dextrose agar.…”

Section: Antifungal Activity Assaymentioning

confidence: 99%

Coordinated control of the type IV pili and c‐di‐GMP‐dependent antifungal antibiotic production in Lysobacter by the response regulator PilR

Shen

Yang

et al. 2021

Molecular Plant Pathology

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ClpP mediates antagonistic interaction of Lysobacter enzymogenes with a crop fungal pathogen

Cited by 7 publications

References 43 publications

Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system

Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system

An intrinsic mechanism for coordinated production of the contact-dependent and contact-independent weapon systems in a soil bacterium

Coordinated control of the type IV pili and c‐di‐GMP‐dependent antifungal antibiotic production in Lysobacter by the response regulator PilR

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