Clubroot disease on Brassica crops in India

Bhattacharya, I.; Dutta, Subrata; Mondal, S. S.; Mondal, Bholanath

doi:10.1080/07060661.2013.875064

Cited by 29 publications

(17 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The erosion of single dominant CR genes in commercial canola cultivars and the emergence of new virulent isolates of P. brassicae have been reported in Canada ( Strelkov et al, 2016 , 2018 ) and Europe ( Oxley, 2007 ; Diederichsen et al, 2014 ; Wallenhamar et al, 2014 ; Zamani-Noor, 2017 ). The erosion of the effectiveness of CR genes has also occurred in cruciferous vegetables in Asia ( Bhattacharya et al, 2014 ; Chai et al, 2014 ). The elevated infection in clubroot-resistant cultivars and volunteers would lead to increased spore load of the pathogen in the soil ( Zamani-Noor and Rodemann, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Disease development is associated with the formation of large galls on the roots of susceptible plants, which interfere with water and nutrient uptake and lead to significant yield losses in Brassica crops ( Hwang et al, 2012 ; Dixon, 2014 ). Yield losses of 20–100% have been reported worldwide including in Canada ( Tewari et al, 2005 ; Rahman et al, 2014 ), China ( Chai et al, 2014 ), and India ( Bhattacharya et al, 2014 ). The clubroot pathogen survives as resting spores that can persist in the soil for many years ( Dixon, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

QTL Mapping and Inheritance of Clubroot Resistance Genes Derived From Brassica rapa subsp. rapifera (ECD 02) Reveals Resistance Loci and Distorted Segregation Ratios in Two F2 Populations of Different Crosses

et al. 2020

View full text Add to dashboard Cite

In this study, Brassica rapa subsp. rapifera (ECD 02) which exhibits broad-spectrum resistance to many Canadian Plasmodiophora brassicae isolates was crossed with two clubroot-susceptible B. rapa accessions to produce two F 2 populations. The F 2 plants were screened against P. brassicae pathotypes 3H, 5X, and 5G. The Chi-square goodness of fit test showed that the vast majority (≈75%) of the crosses that produced the F 2 populations showed segregation ratios of 9R:7S, 7R:9S, 13R:3S, 3R:13S, 5R:11S, 11R:5S, and 1R:15S. These were modifications of the 15R:1S ratio expected for the inheritance of two dominant major clubroot resistance (CR) genes from ECD 02. The distorted segregation ratios suggest that the two resistance genes are on different chromosomes and that two genes interact in an epistatic manner to confer resistance. Genotyping was conducted with 144 PCR-based markers in the two F 2 populations. Linkage and QTL analysis with the polymorphic markers identified two QTLs on chromosome A03 to be associated with resistance to P. brassicae pathotypes 5X and 5G in Popl#1 while only the second QTL on chromosome A03 was associated with resistance to pathotypes 5X and 5G in Popl#2. The QTLs clustered in genomic regions on the A03 chromosome of B. rapa where the CRa / CRb Kato gene(s) are mapped. In addition, the Crr1 gene on the A08 chromosome of B. rapa was detected in the two F 2 populations. Therefore, the phenotypic and molecular data confirm the existence of two CR genes in ECD 02. This is the first study that shows that major dominant genes in Brassica interact in a non-additive manner to confer resistance to different P. brassicae pathotypes. Key Message: This study provides knowledge on the inheritance and type of gene action for clubroot resistance derived from Brassica rapa subsp. rapifera (ECD 02). The results indicated that duplicate recessive and recessive suppression epistatic interactions, digenic additivity and complementary gene action between the CRa / CRb Kato gene(s) on the A03 and the Crr1 gene on the A08 chromosome of B. rapa controlled clubroot resistance to P. brassicae pathotypes 3H, 5X and 5G.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

QTL Mapping and Inheritance of Clubroot Resistance Genes Derived From Brassica rapa subsp. rapifera (ECD 02) Reveals Resistance Loci and Distorted Segregation Ratios in Two F2 Populations of Different Crosses

et al. 2020

View full text Add to dashboard Cite

show abstract

“…However, high world vegetable oil prices over the years have led to intensive cultivation of Brassica oilseed crops with a concomitant dissemination and establishment of the pathogen in many countries. At present, yield losses in excess of 20 % or even total crop failure in high P. brassicae infested fields have been reported in Canada ; for review, see Rahman et al 2014), China (for review, see Chai et al 2014) and India (for review, see Bhattacharya et al 2014). The increasing infestation of Brassica crops by P. brassicae has also been reported in Europe (for review, see Diederichsen et al 2014;Wallenhammar et al 2014) and Australia (for review, see Donald and Porter 2014).…”

Section: Introductionmentioning

confidence: 99%

Mapping of the clubroot disease resistance in spring Brassica napus canola introgressed from European winter canola cv. ‘Mendel’

Fredua-Agyeman

Rahman

2016

Euphytica

View full text Add to dashboard Cite

Clubroot disease caused by the soil-borne pathogen Plasmodiophora brassicae Woronin is a major threat to the production of Brassica crops worldwide. The European winter canola cv. 'Mendel' shows resistance to many P. brassicae isolates including pathotypes 3, 5, 6 and 8 that are prevalent in Canada. To introgress clubroot resistance (CR) into Canadian spring Brassica napus canola, crosses between Canadian spring and European winter B. napus canola cv. 'Mendel' were made and several resistant lines were developed through pedigree breeding. Two of the resistant lines were further crossed with the clubroot susceptible spring canola line A07-26NR to produce two doubled haploid (DH) populations from nine F 1 plants. Segregation for resistance followed a 1:1 ratio for resistant and susceptible phenotypes suggesting that a single Mendelian gene is involved in the control of resistance to P. brassicae single spore isolate SACAN-ss1 (pathotype 3) in the DH population where the 'favourable allele' for resistance is derived from the cv. 'Mendel'. Genetic and physical mapping study positioned five previously described CR loci (CRk, Crr3, CRb, CRa and CRb Kato ) on the B. rapa chromosome A3, and identified twelve markers (1.5-2.0 % recombination) from the genomic region that houses the CRa and CRb Kato loci to be associated with the resistance derived from 'Mendel'. The identified markers can be used in breeding as well as pyramiding of multiple clubroot resistance genes.

show abstract

“…Indeed, the ever-growing rapeseed production all over the world in the past years also increase areas infested by P. brassicae . Dissemination has been observed in Europe, China, India, Canada and Australia (Diederichsen et al 2014; Chai et al 2014; Bhattacharya et al 2014; Rahman et al 2014; Donald and Porter 2014).…”

Section: Introductionmentioning

confidence: 99%

Analysing the genetic architecture of clubroot resistance variation in Brassica napus by associative transcriptomics

Hejna

Havlíčková

et al. 2019

Mol Breeding

View full text Add to dashboard Cite

Clubroot is a destructive soil-borne pathogen of Brassicaceae that causes significant recurrent reductions in yield of cruciferous crops. Although there is some resistance in oilseed rape (a crop type of the species Brassica napus ), the genetic basis of that resistance is poorly understood. In this study, we used an associative transcriptomics approach to elucidate the genetic basis of resistance to clubroot pathotype ECD 17/31/31 across a genetic diversity panel of 245 accessions of B. napus . A single nucleotide polymorphism (SNP) association analysis was performed with 256,397 SNPs distributed across the genome of B. napus and combined with transcript abundance data of 53,889 coding DNA sequence (CDS) gene models. The SNP association analysis identified two major loci (on chromosomes A2 and A3) controlling resistance and seven minor loci. Within these were a total of 86 SNP markers. Altogether, 392 genes were found in these regions. Another 21 genes were implicated as potentially involved in resistance using gene expression marker (GEM) analysis. After GO enrichment analysis and InterPro functional analysis of the identified genes, 82 candidate genes were identified as having roles in clubroot resistance. These results provide useful information for marker-assisted breeding which could lead to acceleration of pyramiding of multiple clubroot resistance genes in new varieties. Electronic supplementary material The online version of this article (10.1007/s11032-019-1021-4) contains supplementary material, which is available to authorized users.

show abstract

Clubroot disease on Brassica crops in India

Cited by 29 publications

References 5 publications

QTL Mapping and Inheritance of Clubroot Resistance Genes Derived From Brassica rapa subsp. rapifera (ECD 02) Reveals Resistance Loci and Distorted Segregation Ratios in Two F2 Populations of Different Crosses

QTL Mapping and Inheritance of Clubroot Resistance Genes Derived From Brassica rapa subsp. rapifera (ECD 02) Reveals Resistance Loci and Distorted Segregation Ratios in Two F2 Populations of Different Crosses

Mapping of the clubroot disease resistance in spring Brassica napus canola introgressed from European winter canola cv. ‘Mendel’

Analysing the genetic architecture of clubroot resistance variation in Brassica napus by associative transcriptomics

Contact Info

Product

Resources

About